KCNMB4 (NM_014505) Human Untagged Clone

CAT#: SC108188

KCNMB4 (untagged)-Human potassium large conductance calcium-activated channel, subfamily M, beta member 4 (KCNMB4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNMB4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108188 sequence for NM_014505 edited (data generated by NextGen Sequencing)
  ATGGCGAAGCTCCGGGTGGCTTACGAGTACACGGAAGCCGAGGACAAGAGCATCCGGCTC
  GGCTTGTTTCTCATCATCTCCGGCGTCGTGTCGCTCTTCATCTTCGGCTTCTGCTGGCTG
  AGTCCCGCGCTGCAGGATCTGCAAGCCACGGAGGCCAATTGCACGGTGCTGTCGGTGCAG
  CAGATCGGCGAGGTGTTCGAGTGCACCTTCACCTGTGGCGCCGACTGCAGGGGCACCTCG
  CAGTACCCCTGCGTCCAGGTCTACGTGAACAACTCTGAGTCCAACTCTAGGGCGCTGCTG
  CACAGCGACGAGCACCAGCTCCTGACCAACCCCAAGTGCTCCTATATCCCTCCCTGTAAG
  AGAGAAAATCAGAAGAATTTGGAAAGTGTCATGAATTGGCAACAGTACTGGAAAGATGAG
  ATTGGTTCCCAGCCATTTACTTGCTATTTTAATCAACATCAAAGACCAGATGATGTGCTT
  CTGCATCGCACTCATGATGAGATTGTCCTCCTGCATTGCTTCCTCTGGCCCCTGGTGACA
  TTTGTGGTGGGCGTTCTCATTGTGGTCCTGACCATCTGTGCCAAGAGCTTGGCGGTCAAG
  GCGGAAGCCATGAAGAAGCGCAAGTTCTCTTAA
  
  Clone variation with respect to NM_014505.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014505 unedited
  TACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGGCGGTGGCGGCGGCGG
  CTCCTCCCGCCCGAGGCAGTCGGGCTCGGCGCCGGGGGCGGGAGGGGGCGGGGGGAGCAC
  GCCAGCCGCCGAGAGTGGGGGGCGATGGCGAAGCTCCGGGTGGCTTACGAGTACACGGAA
  GCCGAGGACAAGAGCATCCGGCTCGGCTTGTTTCTCATCATCTCCGGCGTCGTGTCGCTC
  TTCATCTTCGGCTTCTGCTGGCTGAGTCCCGCGCTGCAGGATCTGCAAGCCACGGAGGCC
  AATTGCACGGTGCTGTCGGTGCAGCAGATCGGCGAGGTGTTCGAGTGCACCTTCACCTGT
  GGCGCCGACTGCAGGGGCACCTCGCAGTACCCCTGCGTCCAGGTCTACGTGAACAACTCT
  GAGTCCAACTCTAGGGCGCTGCTGCACAGCGACGAGCACCAGCTCCTGACCAACCCCAAG
  TGCTCCTATATCCCTCCCTGTAAGAGAGAAAATCAGAAGAATTTGGAAAGTGTCATGAAT
  TGGCAACAGTACTGGAAAGATGAGATTGGTTCCCAGCCATTTACTTGCTATTTTAATCAA
  CATCAAAGACCAGATGATGTGCTTCTGCATCGCACTCATGATGAGATTGTCCTCCTGCAT
  TGCTTCCTCTGGCCCCTGGTGACATTTGTGGGTGGGCGTTCTCATTGGTGGTCCTGACCA
  TCTGTGCCAAGAGCTTGGCGGTCAAGGCGGAAGCCATGAAGAAGCGCCAGTTCTCTTAAA
  GGGGGAGGGAGGGCTGTANAAAGCANAGTACAGAAAGCTGTACTCATCGNCACGCGTCCA
  CCTGNCGAACCTGTGTTTNCTGGGCGCAGAAATNGCCAGGNNCCCNACAGGNCTCTGAGA
  GGCTCATCCCTCAGTGGCACAGAAACAGGCCAACTGNGAGACTTGAACCTCAAAGCTGNA
  TTCTCTGCTGAGCATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014505 unedited
  CTTATTTTATATTTTTAAGTAGCATAAATCATAATGGTTTGTATACTACACTGTACAAAT
  AATATACTGTTACTTTTGAAAGAAATGAACTTCTTATTAGGGTTTTCTGAAAATAGTTAC
  TCCATACAGCTAAGATCTTGACCCTTTAGCCATTGCTACAGCAGATGGAATACAAGCTTT
  GAGGTTCCAAGTCTTCCAGTTGTGCCTGTTTCTGTTGCCACTGAGGGATGAGCCTCTCAG
  AGCCCTGTCGTGGCCCTGTCCATCTCCTGCGCCAGGAAACACAGGTTCCGCAGGTGGACG
  CGTGCCGATGAGTACAGCTTCTGTACTTTGCTTTCTACAAGCCTCCTTCCCCTTTAAGAG
  AACTTGCGCTTCTTCATGGCTTCCGCCTTGACCGCCAAGCTCTTGGCACAGATGGTCAGG
  ACCACAATGAGAACGCCCACCACAAATGTCACCAGGGGCCAGAGGAAGCAATGCAGGAGG
  ACAATCTCATCATGAGTGCGATGCAGAAGCACATCATCTGGTCTTTGATGTTGATTAAAA
  TAGCAAGTAAATGGCTGGGAACCAATCTCATCTTTCCAGTACTGTTGCCAATTCATGACA
  CTTTCCAAATTCTTCTGATTTTCTCTCTTACAGGGAGGGATATAGGAGCACTTGCGGCTG
  GTCAGGAGCTGGTGCTCGTCGCTGTGCAGCAGCGCCCTAAAGTTGGACTCAGAGTTGTCA
  CGTAAACCTGGACCAAGGGTACTGCGAGTGCCCCTGCATCNGCCCCCCAGGTGAAGTGCA
  CTCGAACACCTTGCCAACTGCTTGACCGCAGTACCGTGCATTGGCCTTCGTGGCTTGCAN
  ATCCTGCACGCGGACTTANCCAGCAAAGCCCANATGATAACGACCCACCCCGGAATGATG
  AAACACAGCCAGCCGATGCTCTTGCCTCGGTTCCGGTACTTCGAACCACCGGACTTTCCC
  ATGCCCCAATTCTGGGGTGGTGGGTCCCCCGCCCTTCCCCN
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_014505
• Insert Size: 1280 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014505.4, NP_055320.4
• RefSeq Size: 1631 bp
• RefSeq ORF: 633 bp
• Locus ID: 27345
• UniProt ID: Q86W47
• Cytogenetics: 12q15
• Domains: CaKB
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which slows activation kinetics, leads to steeper calcium sensitivity, and shifts the voltage range of current activation to more negative potentials than does the beta 1 subunit. [provided by RefSeq, Jul 2008]