GALNT12 (NM_024642) Human Untagged Clone

CAT#: SC108103

GALNT12 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 (GalNAc-T12) (GALNT12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALNT12
• Synonyms: CRCS1; GalNAc-T12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024642, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGGGGCGCACGGCGCGGCGGCGCTGCCCGCGGGAACTGCGGCGCGGCCGGGAGGCGCTGTTGGTGC
  TCCTGGCGCTACTGGCGTTGGCCGGGCTGGGCTCGGTGCTGCGGGCGCAGCGTGGGGCCGGGGCCGGGGC
  TGCCGAGCCGGGACCCCCGCGCACCCCGCGCCCCGGGCGGCGCGAGCCGGTCATGCCGCGGCCGCCGGTG
  CCGGCGAACGCGCTGGGCGCGCGGGGCGAGGCGGTGCGGCTGCAGCTGCAGGGCGAGGAGCTGCGGCTGC
  AGGAGGAGAGCGTGCGGCTGCACCAGATTAACATCTACCTCAGCGACCGCATCTCACTGCACCGCCGCCT
  GCCCGAGCGCTGGAACCCGCTGTGCAAAGAGAAGAAATATGATTATGATAATTTGCCCAGGACATCTGTT
  ATCATAGCATTTTATAATGAAGCCTGGTCAACTCTCCTTCGGACAGTTTACAGTGTCCTTGAGACATCCC
  CGGATATCCTGCTAGAAGAAGTGATCCTTGTAGATGACTACAGTGATAGAGAGCACCTGAAGGAGCGCTT
  GGCCAATGAGCTTTCGGGACTGCCCAAGGTGCGCCTGATCCGCGCCAACAAGAGAGAGGGCCTGGTGCGA
  GCCCGGCTGCTGGGGGCGTCTGCGGCGAGGGGCGATGTTCTGACCTTCCTGGACTGTCACTGTGAGTGCC
  ACGAAGGGTGGCTGGAGCCGCTGCTGCAGAGGATCCATGAAGAGGAGTCGGCAGTGGTGTGCCCGGTGAT
  TGATGTGATCGACTGGAACACCTTCGAATACCTGGGGAACTCCGGGGAGCCCCAGATCGGCGGTTTCGAC
  TGGAGGCTGGTGTTCACGTGGCACACAGTTCCTGAGAGGGAGAGGATACGGATGCAATCCCCCGTCGATG
  TCATCAGGTCTCCAACAATGGCTGGTGGGCTGTTTGCTGTGAGTAAGAAATATTTTGAATATCTGGGGTC
  TTATGATACAGGAATGGAAGTTTGGGGAGGAGAAAACCTCGAATTTTCCTTTAGGATCTGGCAGTGTGGT
  GGGGTTCTGGAAACACACCCATGTTCCCATGTTGGCCATGTTTTCCCCAAGCAAGCTCCCTACTCCCGCA
  ACAAGGCTCTGGCCAACAGTGTTCGTGCAGCTGAAGTATGGATGGATGAATTTAAAGAGCTCTACTACCA
  TCGCAACCCCCGTGCCCGCTTGGAACCTTTTGGGGATGTGACAGAGAGGAAGCAGCTCCGGGACAAGCTC
  CAGTGTAAAGACTTCAAGTGGTTCTTGGAGACTGTGTATCCAGAACTGCATGTGCCTGAGGACAGGCCTG
  GCTTCTTCGGGATGCTCCAGAACAAAGGACTAACAGACTACTGCTTTGACTATAACCCTCCCGATGAAAA
  CCAGATTGTGGGACACCAGGTCATTCTGTACCTCTGTCATGGGATGGGCCAGAATCAGTTTTTCGAGTAC
  ACGTCCCAGAAAGAAATACGCTATAACACCCACCAGCCTGAGGGCTGCATTGCTGTGGAAGCAGGAATGG
  ATACCCTTATCATGCATCTCTGCGAAGAAACTGCCCCAGAGAATCAGAAGTTCATCTTGCAGGAGGATGG
  ATCTTTATTTCACGAACAGTCCAAGAAATGTGTCCAGGCTGCGAGGAAGGAGTCGAGTGACAGTTTCGTT
  CCACTCTTACGAGACTGCACCAACTCGGATCATCAGAAATGGTTCTTCAAAGAGCGCATGTTATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024642 unedited
  ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACCAGATTAACATCTACCTCA
  GCGACCGCATCTCACTGCACCGCCGCCTGCCCGAGCGCTGGAACCCGCTGTGCAAAGAGA
  AGAAATATGATTATGATAATTTGCCCAGGACATCTGTTATCATAGCATTTTATAATGAAG
  CCTGGTCAACTCTCCTTCGGACAGTTTACAGTGTCCTTGAGACATCCCCGGATATCCTGC
  TAGAAGAAGTGATCCTTGTAGATGACTACAGTGATAGAGAGCACCTGAAGGAGCGCTTGG
  CCAATGAGCTTTCGGGACTGCCCAAGGTGCGCCTGATCCGCGCCAACAAGAGAGAGGGCC
  TGGTGCGAGCCCGGCTGCTGGGGGCGTCTGCGGCGAGGGGCGATGTTCTGACCTTCCTGG
  ACTGTCACTGTGAGTGCCACGAAGGGTGGCTGGAGCCGCTGCTGCAGAGGATCCATGAAG
  AGGAGTCGGCAGTGGTGTGCCCGGTGATTGATGTGATCGACTGGAACACCTTCGAATACC
  TGGGGAACTCCGGGGAGCCCCAGATCGGCGGTTTCGACTGGAGGCTGGTGTTCACGTGGC
  ACACAGTTCCTGAGAGGGAGAGGATACGGATGNCATCCCCCGTCGATGTCATCAGGTCTC
  CAACATGGCTGGTGGGCTGTTTGCTGTGAGTAAGAAATATTTTGATATCTGGNGTCTTAT
  GATACAGGAATGGNAAGTTGGNGAGGAGAAACCTCGAATTTTCTTTAGGGTGTCAGGCTG
  CTCTACCTGTTNTAGACGCTCTTNCTTTCCCCTTNNCAAACTCTTTCTTNCTTGCTGCNT
  TNCTATCTTCTGTGGCTAGAGATCAGATCTGCAGGTGGGNGGGGGTCTGGAACCACCATG
  TNCCCATGTGGCATGNTTNCCNAGCAGCTNCCTATCCGCACAAGCTCTGGCACATTGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024642
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024642.1, NP_078918.1
• RefSeq Size: 2752 bp
• RefSeq ORF: 855 bp
• Locus ID: 79695
• UniProt ID: Q8IXK2
• Cytogenetics: 9q22.33
• Domains: RICIN, Glycos_transf_2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: This gene encodes a member of a family of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases, which catalyze the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to a serine or threonine residue on a polypeptide acceptor in the initial step of O-linked protein glycosylation. Mutations in this gene are associated with an increased susceptibility to colorectal cancer.[provided by RefSeq, Mar 2011]