CDH20 (NM_031891) Human Untagged Clone

CAT#: SC107114

CDH20 (untagged)-Human cadherin 20, type 2 (CDH20)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDH20
• Synonyms: Cdh7; CDH7L3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107114 sequence for NM_031891 edited (data generated by NextGen Sequencing)
  ATGTGGACTTCTGGTAGAATGAGCAATGCAAAGAACTGGCTTGGACTTGGCATGTCCTTG
  TACTTCTGGGGGCTGATGGACCTTACGACCACCGTTCTCTCGGACACCCCAACACCACAA
  GGTGAATTAGAAGCACTCCTGTCAGACAAGCCACAGTCACATCAGCGGACCAAGAGGAGC
  TGGGTTTGGAACCAGTTTTTCGTTCTGGAAGAGTACACTGGGACCGACCCTTTGTATGTC
  GGCAAGCTTCATTCAGATATGGACAGGGGAGACGGATCCATCAAATACATCCTCTCGGGA
  GAAGGTGCTGGCATCGTGTTTACCATCGACGACACCACTGGAGACATCCACGCCATTCAG
  AGGCTCGATCGAGAGGAAAGAGCCCAGTATACTCTAAGGGCTCAAGCCCTAGACAGGCGG
  ACGGGCAGGCCAATGGAGCCCGAGTCAGAGTTCATCATCAAAATTCAAGACATCAATGAC
  AATGAGCCCAAGTTCCTGGACGGACCTTATGTGGCCACTGTGCCAGAAATGTCCCCTGTG
  GGTACCTCCGTCATCCAAGTGACAGCCACAGATGCAGATGACCCGACCTACGGCAACAGT
  GCCAGGGTGGTGTACAGCATTCTTCAGGGCCAGCCATATTTTTCTGTGGACTCTAAAACA
  GGTGTAATTAGGACAGCGCTCATGAACATGGACAGAGAAGCCAAAGAATACTACGAAGTG
  ATTATCCAAGCCAAGGACATGGGAGGGCAGCTTGGAGGATTAGCTGGGACCACAACAGTG
  AACATCACCCTCTCAGATGTCAATGATAACCCACCCCGCTTTCCCCAGAAACATTACCAG
  ATGAGTGTGTTGGAATCAGCTCCAATTAGCTCCACTGTCGGGAGAGTGTTTGCCAAGGAC
  TTGGATGAAGGCATCAATGCAGAGATGAAATATACTATTGTGGATGGAGATGGTGCAGAT
  GCCTTTGACATTAGCACAGATCCCAATTTCCAAGTTGGTATCATAACTGTGAAGAAGCCC
  CTGAGTTTTGAAAGCAAGAAAAGCTACACCTTAAAGGTGGAGGGAGCCAATCCTCACCTA
  GAGATGCGTTTTCTGAACTTGGGCCCATTTCAGGACACAACAACAGTGCACATCAGTGTG
  GAAGACGTGGACGAGCCCCCTGTGTTTGAACCTGGCTTTTACTTTGTGGAGGTGCCTGAG
  GATGTGGCGATTGGAACAACCATACAGATCATTTCTGCCAAGGACCCAGATGTGACCAAC
  AACTCAATCAGATACTCCATTGATAGAAGCAGTGACCCTGGAAGATTTTTCTATGTTGAC
  ATTACAACAGGTGCCCTAATGACAGCAAGACCCCTAGACCGGGAAGAATTTTCTTGGCAT
  AATATCACTGTCCTTGCTATGGAAATGAACAATCCCTCCCAGGTTGGAAGTGTTCCTGTC
  ACAATCAAAGTCTTAGATGTGAATGACAATGCTCCAGAGTTCCCCAGATTCTATGAAGCT
  TTTGTCTGTGAGAACGCCAAGGCAGGACAGCTGATCCAGACAGTGAGTGCGGTGGACCAA
  GATGACCCACGCAATGGTCAGCATTTCTACTACAGCTTGGCTCCTGAGGCTGCTAACAAC
  CCCAACTTTACCATAAGGGACAACCAAGATAACACAGCACGGATTCTAACCAGGAGGTCT
  GGTTTCCGGCAGCAGGAGCAGAGTGTCTTTCACCTGCCTATCCTGATAGCAGATAGCGGG
  CAGCCCGTGCTGAGCAGCACAGGCACACTGACCATCCAAGTGTGCAGCTGTGATGACGAC
  GGCCACGTCATGTCCTGCAGCCCAGAGGCCTACATGCTCCCAGTCAGTTTGAGCCGGGGC
  GCCCTCATTGCCATCCTCGCCTGCATCTTTGTCCTCTTAGTGCTGGTGTTGCTCATTTTG
  TCCATGAGGCGGCACCGGAAACAACCATACATCATCGACGACGAGGAAAACATCCACGAG
  AACATCGTCCGCTACGACGACGAGGGCGGCGGCGAGGAGGACACCGAGGCCTTCGACATC
  GCGGCCATGTGGAACCCCCGGGAGGCGCAGGCGGGGGCCGCCCCCAAGACGCGGCAGGAC
  ATGCTGCCCGAGATCGAGAGCCTCTCCCGCTACGTGCCTCAGACGTGCGCAGTGAACAGC
  ACTGTCCACAGCTACGTGCTGGCCAAGCTCTACGAGGCCGACATGGACCTGTGGGCACCG
  CCCTTCGACTCCCTCCAGACGTATATGTTCGAGGGGGACGGCTCTGTGGCGGGGTCGCTG
  AGCTCCCTGCAGTCGGCCACGTCGGACTCGGAACAGAGCTTCGACTTCCTGACGGACTGG
  GGGCCCCGCTTCCGGAAGCTGGCCGAGCTCTACGGGGCGTCGGAGGGACCCGCGCCGCTG
  TGGTGA
  
  Clone variation with respect to NM_031891.2
  369 c=>t;780 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031891 unedited
  GGATTTTGTAATACGATTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGCAGCTACG
  TGAGCAGAACGCCCGCCCTGTGACAGTTAGGACCGAAGGTCTCCGGAGAGTCGCCGGCGG
  TGCCAGGAAGTCAACTTCAAGCAGATTGACTTGAAACGGGATCTCATTTAGGAAGCATAA
  GTGTCCAATCAAAAACTGTGTATTTTTTTTTTTTGGAAAATACTCAAGTTCCAGTTGCTT
  ATCATTCTCCTTCACTTTCTGAAAACCTGGCAATCCCATGTGGACTTCTGGTAGAATGAG
  CAATGCAAAGAACTGGCTTGGACTTGGCATGTCCTTGTACTTCTGGGGGCTGATGGACCT
  TACGACCACCGTTCTCTCGGACACCCCAACACCACAAGGTGAATTAGAAGCACTCCTGTC
  AGACAAGCCACAGTCACATCAGCGGACCAAGAGGAGCTGGGTTTGGAACCAGTTTTTCGT
  TCTGGAAGAGTACACTGGGACCGACCCTTTGTATGTCGGCAAGCTTCATTCAGATATGGA
  CAGGGGAGACGGATCCATCAAATACATCCTCTCGGGAGAAGGTGCTGGCATCGTGTTTAC
  CATCGACGACACCACTGGAGACATCCACGCCATTCAGAGGCTCGATCGAGAGGAAAGAGC
  CCAGTATACTCTAAGGGCTCAAGCCCTAGACAGGCGGACGGGCAGGCCAATGGAGCCCGA
  GTCAGAGTTCATCATCAAAATTCAAGACATTAATGACAATGAGCCCCAGTTTCCTGGACT
  GACCTTATGTTGGCCACTGTGCCCAGAAATGTTCCCTGTGGGTACCCTTCGTTATTCCAG
  TGACAGCCCCAGATGCAGATGACCCGACCTACCGCACAGTGCCAGGTTGGTGTACAGAAT
  TCTTAAGGGCAGCCCTATTTTTTGTGGACTCTTAACAGTGTAATAATGCAGCGNCTCTGA
  ACATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031891 unedited
  NTTCTCTCTGGACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTCTATT
  TAACTTTTATTAAAAAAAGTGCTAAGGTACTGAGATGCAGTACATCATATTTATTACCAA
  AATTATTAACAAATATACAAAACTTGTACATGTAATTTTCTTACATCCATTTATTCTCTA
  TAATACTGATTTTTGTTCAAGTAGGTTATCTACAGTATGTAAACATCCCTTGGATTGACC
  TCACACAGATTAGGAAAGCCCTCTAACCCATGCTTTTGTCAGATACTTCTTTTTTATTTG
  GCTTTCATTTGTAGCATCTAATCTCCACATTTGGCACATGGAAGAGACATCTACAGTGCA
  AAAGAGGCAGATTTTTCTGGTTTCATTCTGAACAATTTTGCAGGGGCACAGTTAAATAGC
  TGAGAGGAGACACGAGCACTCTAAGTTGATGGCGTTGGATGAGCTGTAATGCCAACATCT
  CTTGGTTCAGCCACTAAACTTGAGCATAAAGTCAATGTCTTTNNCCTTCACCCACCCTCC
  CACCCATCCACCCCCATGCAAGGCTGATTCAGTCTTAACCTTGTCTCAAAAGGGTTACGG
  GAAATTCCATCCATCCCAGAACAAGTAGGTTAATTTCACTGAGTAACAAGTTTTTTGTTC
  TTTCTTGNGGGGTTGTTTCTGTGCTTTGAGCCAGCAAACTCTTAAATGGAGAATAAGATA
  AGATAAAAAACAGATACGAGAGTAGAACTTTCTGCTTTAATTGGCATCTCTGTATTCAAG
  GCCAGAGCCTTTCTGTGGTGGAAAAGTGACAAAGGCCTTACGAAAGGCAGAAAAAGCCCA
  CCTTGTCAGAACACCTTGGACTCCAACCCTTCGCACACGGGGCCGGAAAATCCAAAAGAA
  AGAAAAAAAGGTATTTAAAATTCTGCCCTCTTCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031891
• Insert Size: 3750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031891.2, NP_114097.2
• RefSeq Size: 2855 bp
• RefSeq ORF: 2406 bp
• Locus ID: 28316
• UniProt ID: Q9HBT6
• Cytogenetics: 18q21.33
• Domains: Cadherin_C_term, CA
• Protein Families: Druggable Genome
• Gene Summary: This gene is a type II classical cadherin from the cadherin superfamily and one of three cadherin 7-like genes located in a cluster on chromosome 18. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Since disturbance of intracellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes. [provided by RefSeq, Jul 2008]