CDH20 (NM_031891) Human Tagged ORF Clone

CAT#: RG214769

CDH20 (tGFP-tagged) - Human cadherin 20, type 2 (CDH20)

Product Images

Circular map for RG214769

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDH20
• Synonyms: Cdh7; CDH7L3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214769 representing NM_031891
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACTTCTGGTAGAATGAGCAATGCAAAGAACTGGCTTGGACTTGGCATGTCCTTGTACTTCTGGG
  GGCTGATGGACCTTACGACCACCGTTCTCTCGGACACCCCAACACCACAAGGTGAATTAGAAGCACTCCT
  GTCAGACAAGCCACAGTCACATCAGCGGACCAAGAGGAGCTGGGTTTGGAACCAGTTTTTCGTTCTGGAA
  GAGTACACTGGGACCGACCCTTTGTATGTCGGCAAGCTTCATTCAGATATGGACAGGGGAGACGGATCCA
  TCAAATACATCCTCTCGGGAGAAGGTGCTGGCATCGTGTTTACCATCGACGACACCACTGGAGACATCCA
  CGCCATTCAGAGGCTCGACCGAGAGGAAAGAGCCCAGTATACTCTAAGGGCTCAAGCCCTAGACAGGCGG
  ACGGGCAGGCCAATGGAGCCCGAGTCAGAGTTCATCATCAAAATTCAAGACATCAATGACAATGAGCCCA
  AGTTCCTGGACGGACCTTATGTGGCCACTGTGCCAGAAATGTCCCCTGTGGGTACCTCCGTCATCCAAGT
  GACAGCCACAGATGCAGATGACCCGACCTACGGCAACAGTGCCAGGGTGGTGTACAGCATTCTTCAGGGC
  CAGCCATATTTTTCTGTGGACTCTAAAACAGGTGTAATTAGGACAGCGCTCATGAACATGGACAGAGAAG
  CCAAAGAATACTACGAAGTGATTATCCAAGCCAAGGACATGGGAGGGCAGCTTGGAGGATTAGCTGGGAC
  CACAACAGTCAACATCACCCTCTCAGATGTCAATGATAACCCACCCCGCTTTCCCCAGAAACATTACCAG
  ATGAGTGTGTTGGAATCAGCTCCAATTAGCTCCACTGTCGGGAGAGTGTTTGCCAAGGACTTGGATGAAG
  GCATCAATGCAGAGATGAAATATACTATTGTGGATGGAGATGGTGCAGATGCCTTTGACATTAGCACAGA
  TCCCAATTTCCAAGTTGGTATCATAACTGTGAAGAAGCCCCTGAGTTTTGAAAGCAAGAAAAGCTACACC
  TTAAAGGTGGAGGGAGCCAATCCTCACCTAGAGATGCGTTTTCTGAACTTGGGCCCATTTCAGGACACAA
  CAACAGTGCACATCAGTGTGGAAGACGTGGACGAGCCCCCTGTGTTTGAACCTGGCTTTTACTTTGTGGA
  GGTGCCTGAGGATGTGGCGATTGGAACAACCATACAGATCATTTCTGCCAAGGACCCAGATGTGACCAAC
  AACTCAATCAGATACTCCATTGATAGAAGCAGTGACCCTGGAAGATTTTTCTATGTTGACATTACAACAG
  GTGCCCTAATGACAGCAAGACCCCTAGACCGGGAAGAATTTTCTTGGCATAATATCACTGTCCTTGCTAT
  GGAAATGAACAATCCCTCCCAGGTTGGAAGTGTTCCTGTCACAATCAAAGTCTTAGATGTGAATGACAAT
  GCTCCAGAGTTCCCCAGATTCTATGAAGCTTTTGTCTGTGAGAACGCCAAGGCAGGACAGCTGATCCAGA
  CAGTGAGTGCGGTGGACCAAGATGACCCACGCAATGGTCAGCATTTCTACTACAGCTTGGCTCCTGAGGC
  TGCTAACAACCCCAACTTTACCATAAGGGACAACCAAGATAACACAGCACGGATTCTAACCAGGAGGTCT
  GGTTTCCGGCAGCAGGAGCAGAGTGTCTTTCACCTGCCTATCCTGATAGCAGATAGCGGGCAGCCCGTGC
  TGAGCAGCACAGGCACACTGACCATCCAAGTGTGCAGCTGTGATGACGACGGCCACGTCATGTCCTGCAG
  CCCAGAGGCCTACATGCTCCCAGTCAGTTTGAGCCGGGGCGCCCTCATTGCCATCCTCGCCTGCATCTTT
  GTCCTCTTAGTGCTGGTGTTGCTCATTTTGTCCATGAGGCGGCACCGGAAACAACCATACATCATCGACG
  ACGAGGAAAACATCCACGAGAACATCGTCCGCTACGACGACGAGGGCGGCGGCGAGGAGGACACCGAGGC
  CTTCGACATCGCGGCCATGTGGAACCCCCGGGAGGCGCAGGCGGGGGCCGCCCCCAAGACGCGGCAGGAC
  ATGCTGCCCGAGATCGAGAGCCTCTCCCGCTACGTGCCTCAGACGTGCGCAGTGAACAGCACTGTCCACA
  GCTACGTGCTGGCCAAGCTCTACGAGGCCGACATGGACCTGTGGGCACCGCCCTTCGACTCCCTCCAGAC
  GTATATGTTCGAGGGGGACGGCTCTGTGGCGGGGTCGCTGAGCTCCCTGCAGTCGGCCACGTCGGACTCG
  GAACAGAGCTTCGACTTCCTGACGGACTGGGGGCCCCGCTTCCGGAAGCTGGCCGAGCTCTACGGGGCGT
  CGGAGGGACCCGCGCCGCTGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214769 representing NM_031891
  Red=Cloning site Green=Tags(s)
  
  MWTSGRMSNAKNWLGLGMSLYFWGLMDLTTTVLSDTPTPQGELEALLSDKPQSHQRTKRSWVWNQFFVLE
  EYTGTDPLYVGKLHSDMDRGDGSIKYILSGEGAGIVFTIDDTTGDIHAIQRLDREERAQYTLRAQALDRR
  TGRPMEPESEFIIKIQDINDNEPKFLDGPYVATVPEMSPVGTSVIQVTATDADDPTYGNSARVVYSILQG
  QPYFSVDSKTGVIRTALMNMDREAKEYYEVIIQAKDMGGQLGGLAGTTTVNITLSDVNDNPPRFPQKHYQ
  MSVLESAPISSTVGRVFAKDLDEGINAEMKYTIVDGDGADAFDISTDPNFQVGIITVKKPLSFESKKSYT
  LKVEGANPHLEMRFLNLGPFQDTTTVHISVEDVDEPPVFEPGFYFVEVPEDVAIGTTIQIISAKDPDVTN
  NSIRYSIDRSSDPGRFFYVDITTGALMTARPLDREEFSWHNITVLAMEMNNPSQVGSVPVTIKVLDVNDN
  APEFPRFYEAFVCENAKAGQLIQTVSAVDQDDPRNGQHFYYSLAPEAANNPNFTIRDNQDNTARILTRRS
  GFRQQEQSVFHLPILIADSGQPVLSSTGTLTIQVCSCDDDGHVMSCSPEAYMLPVSLSRGALIAILACIF
  VLLVLVLLILSMRRHRKQPYIIDDEENIHENIVRYDDEGGGEEDTEAFDIAAMWNPREAQAGAAPKTRQD
  MLPEIESLSRYVPQTCAVNSTVHSYVLAKLYEADMDLWAPPFDSLQTYMFEGDGSVAGSLSSLQSATSDS
  EQSFDFLTDWGPRFRKLAELYGASEGPAPLW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031891
• ORF Size: 2403 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031891.4
• RefSeq Size: 2855 bp
• RefSeq ORF: 2406 bp
• Locus ID: 28316
• UniProt ID: Q9HBT6
• Cytogenetics: 18q21.33
• Domains: Cadherin_C_term, CA
• Protein Families: Druggable Genome
• Gene Summary: This gene is a type II classical cadherin from the cadherin superfamily and one of three cadherin 7-like genes located in a cluster on chromosome 18. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Since disturbance of intracellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes. [provided by RefSeq, Jul 2008]