PHPT1 (AF131857) Human Untagged Clone

CAT#: SC106263

(untagged)-Human clone 24870 mRNA sequence

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHPT1
• Synonyms: CGI-202; HEL-S-132P; HSPC141; PHP14
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AF131857, the custom clone sequence may differ by one or more nucleotides
  
  
  GGCCTCCCTCGTTGCCCCAGCCTCGCGGGCCGCCTAACTGCCCCGTTCCAAGGGTGCCACCGGACCCCGC
  TGGAGAGGAACTTCTCCGTTGGCTGATTTCATCACCACCCATTCCCGATTCCACGTTTCCTTTAAGCGGG
  GCTGGCGGAGCCGCAAGGCGGCAAGGAACTGGATTGCGATTGGTCAGCACGTGCCTCGGTCGGCGGTACA
  ATTGGCTGAGGCGCTGGGCCTTGGGAAGCATTCCCCGACGGGATTGGTCGTCGCTCTCGCAGAGCCCGCC
  TCCCGCAGTACAAGCGGCCCCCGGGTCGGGTGGGAGGAGGGGACTCCGGGAGGAGGAACATGGCGGTGGC
  GGACCTCGCTCTCATTCCTGATGTGGACATCGACTCCGACGGCGTCTTCAAGTATGTGCTGATCCGAGTC
  CACTCGGCTCCCCGCTCCGGGGCTCCGGCTGCAGAGAGCAAGGAGATCGTGCGCGGCTACAAGTGGGCTG
  AGTACCATGCGGACATCTACGACAAAGTGTCGGGCGACATGCAGAAGCAAGGCTGCGACTGTGAGTGTCT
  GGGCGGCGGGCGCATCTCCCACCAGAGTCAGGACAAGAAGATTCACGTGTACGGCTATTCCATGGTGAGC
  CGCAGCCCCGTCCCGCCCTGCCGGAGGCCCCAGTACCAGCTTCGAGGCCCACCTGAGCCTGCTGCCCTGA
  CCCGTGGCCCCAGCTGAGCACGCAGGCTTCCTGGGGTTCTCCCAGGGTCGGCGGCAGAGCCCTCCCTCCA
  GGGCCCATTGTGTTCCTGCATTCCCCCATGGAGCACACGCCAGACCTGAGGGGTGGGACGGACACCCCCA
  GGCATGGCCGGCTGTCTCCTCTCCCTGCCTTGGGAGGCCTTGCTGGGCTCTAGCTGTCCTCCAGCACTTT
  GGGCCCTGGGCCCCCAGAGGCAGTCAGTACCTGGGTGGAGCTCAGAGTCCCCACCTGTGCTCTTCACAAA
  AACCACCAGCAGATGAGACCCACGTGCGTCCCTCTGGGCGCCTCAGGCCCCAGGATCCACCATCAAGGCC
  TATGGTCCTGCCCAGCACGCCATTTCAACTGAGAAAATCAAAGCCAAGTACCCCGACTACGAGGTCACCT
  GGGCTAACGACGGCTACTGAGCACTCCCAGCCCGGGGCCTGCTGCCTCCAGCAGCCACTTCAGAGCCCCC
  GCCTTTGCCTGCACTCCTCTTGCAGGGCTGGCCCTGCCTGCTCCTGCGGCAGCCTCTGGTGACGTGCTGT
  CCACCAGGCCTTGGAGACAGGCTAGCCTGGCCACAGAATTAAACGTGTTGCCACACCAAAAAAAAAAAAA
  AAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF131857 unedited
  GTAACTGGCAACACCCTACCCTTCCCCNNCGCGGTTCGGATTTGTAAACGACTCACTATA
  GGGCGGCCGCGAATCGGCACCAGGGCAAGTCCCTCAGGCCTCCCTCGTTGCCCCAGCCTC
  GCGGGCCGCCTAACTGCCCCGTTCCAAGGGTGCCACCGGACCCCGCTGGAGAGGAACTTC
  TCCGTTGGCTGATTTCATCACCACCCATTCCCGATTCCACGTTTCCTTTAAGCGGGGCTG
  GCGGAGCCGCAAGGCGGCAAGGAACTGGATTGCGATTGGTCAGCACGTGCCTCGGTCGGC
  GGTACAATTGGCTGAGGCGCTGGGCCTTGGGAAGCATTCCCCGACGGGATTGGTCGTCGC
  TCTCGCAGAGCCCGCCTCCCGCAGTACAAGCGGCCCCCGGGTCGGGTGGGAGGAGGGGAC
  TCCGGGAGGAGGAACATGGCGGTGGCGGACCTCGCTCTCATTCCTGATGTGGACATCGAC
  TCCGACGGCGTCTTCAAGTATGTGCTGATCCGAGTCCACTCGGCTCCCCGCTCCGGGGCT
  CCGGCTGCAGAGAGCAAGGAGATCGTGCGCGGCTACAAGTGGGCTGAGTACCATGCGGAC
  ATCTACGACAAAGTGTCGGGCGACATGCAGAAGCAAGGCTGCGACTGTGAGTGTCTGGGC
  GGCGGGCGCATCTCCCACCAGAGTCAGGACAAGAAGATTCACGTGTACGGCTATTCCATG
  GTGAGCCGCAGCCCCGTCCCGCCCTGCCCNAGGCCCCAGTACCAGCTTCGAGGCCCACCT
  GAGCCTGCTGCCCTGACCCGTGGCCCCAGCTGAGCACGCANGCTTTCTGNNNGTTCTCCA
  GGNTCCGCGGCAGAGCCCTCCCTCCCAGGCCCAATGTGTTTCCGCCTTCCCCCAGGNAGC
  ACACCCCANACCTGAGGGGTGGGGACCGGACACCTCCAAGNCATGGCCCGGCTGTTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: AF131857
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF131857.1
• RefSeq Size: 1338 bp
• RefSeq ORF: 1338 bp
• Locus ID: 29085
• Cytogenetics: 9q34.3
• Domains: Ocnus
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways, Riboflavin metabolism, Thiamine metabolism
• Gene Summary: This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]