RPL28 (BC011582) Human Untagged Clone

CAT#: SC105935

RPL28 (untagged)-Homo sapiens, clone MGC:20378 IMAGE:4561118, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL28
• Synonyms: 60S ribosomal protein L28; FLJ43307; ribosomal protein L28
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC011582, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGCGCATCTGCAATGGATGGTCGTGCGGAACTGCTCCAGTTTCCTGATCAAGAGGAATAAGCAGA
  CCTACAGCACTGAGCCCAATAACTTGAAGGCCCGCAATTCCTTCCGCTACAACGGACTGATTCACCGCAA
  GACTGTGGGCGTGGAGCCGGCAGCCGACGGCAAAGGTGTCGTGGTGGTCATTAAGCGGAGATCCGGCCAG
  CGGAAGCCTGCCACCTCCTATGTGCGGACCACCATCAACAAGAATGCTCGCGCCACGCTCAGCAGCATCA
  GACACATGATCCGCAAGAACAAGTACCGCCCCGACCTGCGCATGGCAGCCATCCGCAGGGCCAGCGCCAT
  CCTGCGCAGCCAGAAGCCTGTGATGGTGAAGAGGAAGCGGACCCGCCCCACCAAGAGCTCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC011582 unedited
  GCCGCGAATTCGGCACCAGCCGCTGCGGAGGGAGCCGCCGCCATGTCTGCGCATCTGCAA
  TGGATGGTCGTGCGGAACTGCTCCAGTTTCCTGATCAAGAGGAATAAGCAGACCTACAGC
  ACTGAGCCCAATAACTTGAAGGCCCGCAATTCCTTCCGCTACAACGGACTGATTCACCGC
  AAGACTGTGGGCGTGGAGCCGGCAGCCGACGGCAAAGGTGTCGTGGTGGTCATTAAGCGG
  AGATCCGGCCAGCGGAAGCCTGCCACCTCCTATGTGCGGACCACCATCAACAAGAATGCT
  CGCGCCACGCTCAGCAGCATCAGAACACATGATCCGCAAGAACAAGTACCGCCCCGACCT
  GCGCATGGCAGCCATCCGCAGGGCCAGCGCCATCCTGCGCAGCCAGAAGCCTGTGATGGT
  GAGAGGAAACCCGACCCCCCCCACCAAGAGCTCCTGAGCCCCCCTGCCCCCGAGCAATAA
  AGTCAGCCTGGCTTTCTCNNNNANAAANNNNNNNNNANAAAAAAAAAAAAANAACCTCGA
  CTCTAGATTGCGCCCGGGTCATAGCTGTTCCTGAAAAATCCCGGGGGGCATTCTTGGAAC
  CCCTCCCAAGGCCTTTCCTGGCCCTGAAGTTGCCCTCCAGGGCCACCACCCCTTGTCTAA
  AAAATTAAGTGCATATTTGCTGAAAGGGGTCTCTAATAGGGGGGGGGGGGGGGGGNNA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC011582 unedited
  NNNCGGTAAAAGCTAGNAACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTGAAAAAACCAGCTGACTTTATTGCTCTGGGGGCAGG
  GGGCTCAAGAACTCTTGGTGGGGCGGGTCCCCTTCCTCTTCACCATCACAAGCTTTTGGC
  TGCCCAAGATGGCGCTGGCCCTGCGGATGGCTGCCATGCCCAAGTCGGGGCGGTACTTGT
  TCTTGCGGATCATGTGTCTGAAGCTGCTGAACGTGGCCCCAGCATTCTTGTTGATGGGGG
  TCCCCACATAAGAAGTGGCAGGCTTCCCCTGGCCGGATCTCCGCTTAATGACCACCACGA
  CACCTTTGCCGTCGGCTGCCGGCTCCACGCCCACAGTCTTGCGGTGAATCAATCCCTTGT
  AACCGAAAGAATTGCGGGCCTTCAAGTTATTGGGCTCAATGCTGTAAGTCTGCTTATTCC
  TCTTGATCAAGAAACTGGAGCAATTCCCCACGACCATCCATTGCAAATGCCCAAACATGG
  GGGCGGCTCCCTCCACAACGGCTGGTGCCCAATTCCCGGCCGCCCTATAGTGAGTCCTAT
  TACAAAATTCTGACGGTTCACTAAACGAACTCTGCTTATATAAACCTCCCACCGTACACG
  CCTACCGCCCATTTGCGTCAACGGGGGCGGGGTTATTACCACATTTTGGAAAGTCCCGTT
  GATTTTGGTGCCCAAACAAACTCCCATTGACGTCAATGGGGGTGGAGACTCTGGAAATCC
  CCGTGAGTCAAACCGCTATCCACGCCCATTTGTTGTACTGCCAAAACCGCATCACCATGG
  TAATAAGCGATGACTAATACCGTAAATGTACTGCCCAGTATAGAAAGTCCCGGTTAGGTC
  ATGTTACTGGGCATAATGGCCAGGCGGGGCCATTTACCGTCATTGGACGTCA
  
• Restriction Sites: NotI-NotI
• ACCN: BC011582
• Insert Size: 600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011582.1, AAH11582.1
• RefSeq Size: 4222 bp
• RefSeq ORF: 414 bp
• Locus ID: 6158
• Cytogenetics: 19q13.42
• Domains: Ribosomal_L28e
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]