HSCB (BC000004) Human Untagged Clone

CAT#: SC105817

(untagged)-Homo sapiens, clone MGC:2637 IMAGE:3505128, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSCB
• Synonyms: DNAJC20; HSC20; JAC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC000004, the custom clone sequence may differ by one or more nucleotides
  
  
  GGCACGAGGTCTGGTTAGACGCTCTCTTTGCTTTTCCCCACGAGTGACCACGGCTAGATAGGCCGCCGGC
  CAGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAA
  GGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGCGG
  CCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCC
  ACTCGAGACTACTTCAGCCTTATGGACTGCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACA
  GGTACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTT
  CTCAGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTG
  TACCTTCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATTCCTCA
  TAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATC
  CATTGTCAAAGCTAAACAGAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAA
  GAAGCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAGTTAAAGAAGA
  TTCCCCTTTAATTGTGGATAGTTTAAAGTTTAAAAAATAAAGTTCTTGCTGGGCACAGTGGCTCACACCT
  GTAATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATGACAAGGTCAGGAGTTCAAGACCAGCTTGGCCA
  ACATAGTGAAACCCCGTCTCTGCTGAAAATACAAAAATTAGCCGGGCATGGTGGCGCGTGCCTGTAATCC
  CAGCTACTTGGTAGGCCGAGGCAGGAGAATCGCTTAAACCCGTGAGGTGGAGGTTGCAGTGAGCAGAGAT
  CACGCAACTGCACTCCAGCTTGGGCAACAGAGTGAGACTTAATCTTGAAAAATAAATAAATGAAAAATAA
  AAAAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC000004 unedited
  CGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACGGCTAGATAGGCCGCCGGCC
  AGATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGG
  TTCCCAGAAGGAGACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCC
  GCTGTTGGAACTGCGGCGGCCCATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCAC
  AGTGCCGAGCGCTGCAGGCACCTGACCCCACTCGAGACTACTTCAGCCTTATGGACTGCA
  ACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGTACCAGCAACTGCAGCGTC
  TTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTTCTCAGAGAAGC
  ATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGT
  ACCTTGTAAGCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGA
  CAGGCAATTCCTCATAGAAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGA
  AGCTGCCATGAAAGAGATTGAATCCATTGTCAAAGCTAAACAGAAAGAATTTACTGACAA
  TGTGAGCAGTGCTTTTGAACAAGATGACTNTGAAGAAGCCAAGGAAATTTTGANCAAGAT
  GAGATACTTTTCAATATAGAAGAANAGATCAGTTAAAGAAGATTCCCCTTTAATTGTGGA
  TAGTTTAAAGTTTAAAAATAAGTTCTTGCTGGGCACAGTGGCTCACACCTGTAATCCCAG
  CACTTTGGGAAGGCTGAGTGGGTGGATGACAAGGTCANGGAGTCAAGACCAGCTGGCACT
  TTAANTGAAAACCCGTCTCTGCTGAAATACAAAATAGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC000004 unedited
  CAATCCCCTGCACCGCGGCCCGCAATTTACAGGCGGTTTTTTTTTTTTTTTTTTCATTTA
  TTTACTTTTTAAGATTACCTCTCACTCTGTTGCCCAAGATGGAGTGCAGCTGCGTGACCT
  TTGATCACTGAAACCTCCACCTAACGGAATTAAGCGACTCTCCTGCCTCGCCCTACCACG
  AAGCTGGAAATACAGGCCCGCGCCACCATGCCCGGCTAATTTTTGCATTTTCAGCAGAGC
  ACGGAGTTAAACTATGCCGCCCAAGTTGGTCTTGCCCTCCCGACCTTGCCATCCACCCAC
  CTAAGCCTCCCACAGCGCTGGCACCACCGGTGTGAGCCACTTGTGCCCACGCAAGCACCT
  TATTTTTCTACCTTCTAACCTATTCCCACTTAAAGGGGACCTCTCTTTAACTTGATCTTT
  CCTTCTATATCTCGCACGCATCCCATCTTTCGCCACAACTCTTCCGCCTTTCTCACGTCC
  TCCCGCCCAAAAGCACTGCTCACATCGCCCCGACCATTCTTTTTGGTCAGCATCTGCAGC
  GAAACCAACCTCTTTCATGCTAGCTTCACCTCCACCTCTCGCGCGCTACTCCTCGCCTCC
  CCATACCCCAACCGAGGAACCCGCCCGCCAATCACCTAACCNGCACTTTAAGGCCACCTA
  CACTCTAGGACGTCTCTCACCCACCACCATCGATCTTCCGCAAACGTAGGCATCACTCGC
  CACCCACCCCTATTCCTCACCACCCTCGACTGATCTTTTCCCCATCCCTCTATGCTCCTA
  ACCCCCATACTTTACCCCTCCGTCCTCCTCTTCCACGCTCTTTCTTTCACTCCACCCTCT
  AACACTCCACTCTCAATCCCTTCCTATCCTTTCAAACATTCCATTCCTTCTTCCCCCCCC
  TCTCTCTCCTTTCTCTGCTATTTATTTCTCCATCCCAACCTCTCCCTCCTCATCCTC
  
• Restriction Sites: NotI-NotI
• ACCN: BC000004
• Insert Size: 1080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC000004.1
• RefSeq Size: 1138 bp
• RefSeq ORF: 1138 bp
• Locus ID: 150274
• Cytogenetics: 22q12.1
• Domains: DnaJ
• Gene Summary: This gene encodes a DnaJ-type co-chaperone and member of the heat shock cognate B (HscB) family of proteins. The encoded protein plays a role in the synthesis of iron-sulfur clusters, protein cofactors that are involved in the redox reactions of mitochondrial electron transport and other processes. Cells in which this gene is knocked down exhibit reduced activity of iron-sulfur cluster-dependent enzymes including succinate dehydrogenase and aconitase. The encoded protein may stimulate the ATPase activity of the mitochondrial stress-70 protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]