RPL10A (AK092386) Human Untagged Clone

CAT#: SC105668

(untagged)-Human cDNA FLJ35067 fis, clone PEBLM2000502

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL10A
• Synonyms: Csa-19; CSA19; L10A; NEDD6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK092386, the custom clone sequence may differ by one or more nucleotides
  
  
  CTGTGTGTGTCCTGGGGGACCAGCAGCACTGTGACGAGGCTAAGGCCGTGGATATCCCCCACATGGACAT
  CGAGGCGCTGAAAAAACTCAACAAGAATAAAAAACTGGTCAAGAAGCTGGGTGAGTCCGGCCGCTGTGGT
  TTTGCATGTGAGATGTGTGGTGGGGGCGGTAGAAAGGCTTTTCTGCCATTTTCGATTTTTAAATGATGAG
  GGGCCTAGAATAGCAAAGGATCGGCGGTGGTTGCCTAGCTTGCCTGAGTGCTGTTTTAGCTTTGGGGTGG
  TTTGATGTTTGTATTGCTATGAGGATTCCAGTTGATGAGGGAGGCCAGGCATTGTAAGTTGACCAGCCAG
  GTGCTGGTGAACTATGATTTGGAAATCTTTACGCTGCGTTGTTTAGGCAGTGGCATTAGACTGCTTTTAC
  AGGTAGGAAGCAGACATTCCCAGTTGTCACGTGTCCAGGGTCCACAGCTAAGAAATAGGCAGAATTCGAG
  CCCAGGCAGTCTTGACCAGAGCATCCGTTGTACAGCGGTTATGCCATGGATCATGGGTCAGCCTGGTTTT
  CTGAGAGCATGTTTTGGGACCAGTGAAGGTCCAGAGGACTTTCTGGAAACCGTTTGGGCTAGGGAAAAGA
  CTGAGGCGGGGTCTTAGAGAGGGTGCTGCTTGGAGGTCACTTACATGATTGATTCAAGTGCGTTTCTGGC
  CTGCCACATTTGAGGTGTGCCTTGGTGACCAGGTTCTAAGCAATGCCAATGGCTTCCTCTCTCTATCAGC
  CAAGAAGTATGATGCGTTTTTGGCCTCAGAGTCTCTGATCAAGCAGATTCCACGAATCCTCGGCCCAGGT
  TTAAATAAGGCAGGAAAGTTCCCTTCCCTGCTCACACACAACGAAAACATGGTGGCCAAAGTGGATGAGG
  TGAAGTCCACAATCAAGTTCCAAATGAAGAAGGTGAGTGGGTCTGGCGGGTTGCTATGGGTGAAGGTGTT
  GGCAGGGTCTAAATCTTATCCAAGTCTCTAAATATGCCAGTAAGAGCACCCACCAGGATTGAAACTTTTG
  GAGTAACCCTGGTCTTGGCCCGGGTCCAAGTACCTGCTCACCAGGCCACTGGGGGAGGAAGGACAGGCCA
  TCTGCTATTCGTCCACCAACCTGACTTGATCCTCTCTTCCCTCCTCCCAGGTGTTATGTCTGGCTGTAGC
  TGTTGGTCACGTGAAGATGACAGACGATGAGCTTGTGTATAACATTCACCTGGCTGTCAACTTCTTGGTG
  TCATTGCTCAAGAAAAACTGGCAGAATGTCCGGGCCTTATATATCAAGAGCACCATGGGCAAGCCCCAGC
  GCCTATATTAAGGCACATTTGAATAAATTCTATTACCAGTT
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK092386 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGAGAAGCCATG
  AGCAGCAAAGTCTCTCGCGACACCCTGTACGAGGCGGTGCGGGAAGTCCTGCACGGGAAC
  CAGCGCAAGCGCCGCAAGTTCCTGGAGACGGTGGAGTTGCAGATCAGCTTGAAGAACTAT
  GATCCCCAGAAGGACAAGCGCTTCTCGGGCACCGTCAGGTTGGCACCGTTCTGATCCCAC
  CCAGCCCTCAGTGCCCCCGTGCTTGCCCCTCCCCTGCAGGCTCCCGCTGAGCCGGAGGCG
  GGCACGTCGGTACTGATGTGCTAGGGTAGTTCAGACCCCCTGCTCCGGGCAGGCGCGGCT
  GGACGGACCCCCACCCTGGGTCTTAAAACATGAGGGGAGGCGTGGGTAGGCCTCGGCCGA
  GCCCGCCGGCCAGCCTGAGAAGCCAGGCTGGCTGCTGGTGAATGTGAACGCTCCGGGTAA
  GGCTCGGTGGCTGCTGCGGTCCCAGACCCTTCACCGGCCCTTGGGACCCAGGGCTAGGCG
  TAACCTGTTGGTGCTGTCCCCCAAAACGCAGGCTTAAGTCCACTCCCCGCCCTAAGTTCT
  CTGTGTGTGTCCTGGGGGACCAGCAGCACTGTGACGAGGCTAAGGCCGTGGATATCCCCC
  ACATGGACATCGAGGCGCTGAAAAAACTCAACAAGAATAAAAAACTGGTCAAGAAGCTGG
  GTGAGTCCGGCCGCTGTGGTTNTGCATGTGAGATGTGTGGTGGGGGCGGTAGAAAGGCTT
  TTCTGCCATTNTCGATTTTAAATGATGAGGNGCCTAGAATAGCANAGGATCCGCGGNGGN
  TGCCTAGCTTGGCTGAGTGCTGGTTAGCTTTGGGGTGGNTNGATGNNTTGATGCTATGAG
  GATCCCAGTGTGAGGGAGGCCNGCATTGTAGGTGACAGCCCAGTGCTGTGACTATGATTG
  GAATCTTACCCTGGCGTGTAGCACGGCATAGATGTTTTAGG
  
• Restriction Sites: NotI-NotI
• ACCN: AK092386
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK092386.1
• RefSeq Size: 1371 bp
• RefSeq ORF: 1371 bp
• Locus ID: 4736
• Cytogenetics: 6p21.31
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]