PDPK1 (AK056253) Human Untagged Clone

CAT#: SC103915

(untagged)-Human cDNA FLJ31691 fis, clone NT2RI2005628

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDPK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK056253, the custom clone sequence may differ by one or more nucleotides
  
  
  TCTAAATTTTGCTGAAAACAAAATGAACGAATGAATGTATATGATCCTTACAGGCTGACAACCAGGGCTA
  CAGGCCTAAAGCTCACAAGCAACAAGTGTTGGCTGGACACAGTAGGGATTCACCACCTCACATCTCCATG
  TCATTCTAAGTCGTTTTTTAACGAAGTAAAATCTATCATTTTTAACGCACAAAATGAAAGCTATGCACAC
  TTCTGAGCTGACTTTATCAGAAAGTCATGAAAAACGAGACCAGAAAGAAGTACACACAGAAGCTACAACT
  GAAAAAGATAAAAAGCATCAGTCAAGATGGTGAAAAGAATCATTAGCTCGCCAGGAAAGACACGCAACCA
  TTGCATGTGGTATTGGCAGGAGAGCCTCTCCACGTCAGCGCTCCGTGGACGTGTGGCCTCGCCTTGGCAC
  TCAGGCTGTGGCACAGGCAGGCATCTCAGTCCCACCCATCCAGGGAGCACTCCAACCTGGCTGACCTCAA
  GGGCGGGGGCCATCTGGGCATCAGCCGTGGCCTGTGAGCCAAACGGTCACTGGCCCTGATCATCAAACTG
  ACGTCCTGCAAACCCACCCAGCCACCCCCGCGGTTACCTGTGAATGATGCCCTTGCCGTGCAAGTACTCT
  AAAGCAGACACAATCTCAGCCGTGTAAAATCGGGTACAGGTCTCATCGAATGAACCGATTTTGCGAATAT
  ATTTAAGTAGTTCTCCATTTTTGGCATAACTAAGGCCGAAATCTGAATATCGTGCAGTTAAGGAAGTGTA
  AAAATCTCTTGTTTCGTTTTGGAGCAGGTTTACTCCGTTGCCCAGACTGGAGTGCAGGGGCACAATCATA
  GCTCACTGCAGCCTCAACCTCCCTGGCTCAAGCGATCCTCCCACCTTAACCAACCCAAGTGGCTGGGAAC
  GTAGGTACGCGCTACCACGCCTGGCCTAGGAGAAGCCACAGCAAACCCCACGTTCCCCGCCACAAAGAGA
  AGGAAGTCCAGAGTCAGTGCCAGGCTGCCACGGCTCAGGGGCCCAGCCCACCACAGCCTTTCATGCCCCC
  CCACACACTCCTGCCCAGGAGCTGAAAGAGCCCCACACTGCCGCCAGCCCCTACCCAGCCCTAAGACTCT
  TGGCAGCACATCTTGCTGCCGGGAAGCCTCTGACACGGATCGTCAGTGCACGTCCAGCTCCTCCACCAAA
  ATCGAAGCTTCTCGTGGGCAGAGACACCACCCGGCATAGCAGCGCATCCCCATCACCCATCAACCTGCAC
  TTGGCAAGCACCTCCAAACAGAGAGAGCACACACACTCCGCCGGCAGCCGAAGGAGCTGCAGGATGGTGC
  TGAGAGTGGGAGCAGGCCAGAACGAAGCTCTAACACAGAAGAGCCGGGTGCTGGGGAGAGACGGGGAGGA
  CAGGTGGGAGGACTCAGGCCCCTCCCCAGGCAGGATGGGGAGGCCACGACACTTGGGCCAGCTTGGAGGG
  TGGCGGGGGAGGAGAAGAGCAGATGCAGACTGCACCTGCTGGGGGTGACGACGGTGCGGCGTGGCCAGCC
  CAGCCACTGGCAGGCCCACAGGTCAGCTGGATGGGGCAGAGGTGGGGCCCACCCCAACTTCCACCGGGCC
  TTGCCTCCCAGATTCCTGAGCCAAGGTTTAATAACAGAAAAGATGGAGCTCTAGGGGAGCAAGGGACGCC
  GACCAAGCAAGCCGCAGCAGAGAGGACTGTGCTGGAGCCACATCGGTGGCTTCTCCGGGAGGTAACGTCC
  TGTGCAGACTCCCAGCCACACCCTGGCGCTGCCTCGGCTGCCTCCCTGAATGTCAGCGGCCTGAGGGACC
  CCACTCGGCAGGGAGCGGGGGCTGCTTGTGGGAACACACAGGGTCTGATTCCAAGTGAGAGGGGTGACTG
  GTGTGGCTTCAGACGGCACCAACCACGCAAAGGATACACAGCTTCTCGTCGTCCTGAAATGTGAAGTAAA
  GCTTAACAAAGAAGGGGTGATCCAGGCGCGACATGACATCCCGCTCTCTGGTTACATAGGGGACCTTGTT
  CTCTTTTATGATATGTCGCTTCTCCAGAATTTTAACTTCAGGTGAGAGAGAAGTGAGTTACTATCAGAAA
  CAACAAAAAACACTAAAGACATGACTCACAAAGGTAACTGGTACAAATTAAAGTCTTTCAAACATTGTAC
  ACAACAGCCTGGTGGTCTCTAAAGCCAACAGTGTCCTGTACCCTGAAATCAGCACAGAAACACCGGCCCT
  GCCACCCCAGCCGCCCTGCACGGAGCCGCTTGCCCTGCTCCCGGACGCACAGCTCCCTGCAGCCCATACT
  CACTCGCATATTCTCTGGAGGTTGCCAGTTCTCGAGCCAGGACAACCTGGTTGGG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK056253 unedited
  GGGTTCAAAATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTCA
  GTGCCAGGCTGCCACGGCTCAGGGGCCCAGCCCACCACAGCCTTTCATGCCCCCCCACAC
  ACTCCTGCCCAGGAGCTGAAAGAGCCCCACACTGCCGCCAGCCCCTACCCAGCCCTAAGA
  CTCTTGGCAGCACATCTTGCTGCCGGGAAGCCTCTGACACGGATCGTCAGTGCACGTCCA
  GCTCCTCCACCAAAATCGAAGCTTCTCGTGGGCAGAGACGCCACCCGGCATAGCAGCGCA
  TCCCCATCACCCATCAACCTGCACTTGGCAAGCACCTCCAAACAGAGAGAGCACACACAC
  TCCGCCGGCAGCCGAAGGAGCTGCAGGATGGTGCTGAGAGTGGGAGCAGGCCAGAACGAA
  GCTCTAACACAGAAGAGCCGGGTGCTGGGGAGAGACGGGGAGGACAGGTGGGAGGACTCA
  GGCCCCTCCCCAGGCAGGATGGGGAGGCCACGACACTTGGGCCAGCTTGGAGGGTGGCGG
  NGGAGGAGAAGAGCAGATGCAGACTGCACCTGCTGGGGGTGACGACGGTGTGGCGTGGCC
  AGCCCAGCCACTGGCAGGCCCACAGGTCAGCTGGATGGGGCAGAGGTGGNGCCCACCCCA
  ACTTCCACCGGGCCTTGCCTNCCAGATTCCTGAGCCAGGTTTAAAATACAGAAAGATGGC
  GCTCTANGGGAGCAAGGGACGCCGACCAAGCAAGCCGCAGCAGAGAAGACTGTGCTGGNA
  GCACATCGGTGGCTTCTCCGGGAGGTACGTNCTGTGCAGACTCCCACCACACCCTGGCGC
  TGNNCTCGCTGNCTNCCTAATGTCANNCGGCTGAGGGACCCCACTCGCAGGNANCGGNGG
  CCNTGCTGTGGAAACCACAGGNTCTGATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AK056253 unedited
  ACTATGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTTTCCCAA
  CCGGTTGTCCTGGCTCGAGAACTGGCAACCTCCAGAGAAATGCGAGTGAGTATGGGCTGC
  AGGGAGCTGTGCGTCCGGGAGCAGGGCAAGCGGCTCCGTGCAGGGCGGCTGGGGTGGCAG
  GGCCGGTGTTTCTGTGCTGATTTCAGGGTACAGGACACTGTTGGCTTTAGAGACCACCAG
  GCTGTTGTGTACAATGTTTGAAAGACTTTAATTTGTACCAGTTACCTTTGTGAGTCATGT
  CTTTAGTGTTTTTTGTTGTTTCTGATAGTAACTCACTTCTCTCTCACCTGAAGTTAAAAT
  TCTGGAGAAGCGACATATCATAAAAGAGAACAAGGTCCCCTATGTAACCAGAGAGCGGGA
  TGTCATGTCGCGCCTGGATCACCCCTTCTTTGTTAAGCTTTACTTCACATTTCAGGACGA
  CGAGAAGCTGTGTATCCTTTGCGTGGTTGGTGCCGTCTGAAGCCACACCAGTCACCCCTC
  TCACTTGGAATCAGACCCTGTGTGTTCCCACAAGCAGCCCCCGCTCCCTGCCGAGTGGGG
  TCCCCTCAGCCGCTGACATTCAGGGAGGCAGCCGANGCAGCGCCAGGGTGTGGCTGGGAG
  TCTGCACAGGACGTTACCTCCCGGAGAAGCCACCGATGTGGCTCCAGCACAGTCCTCTCT
  GCTGCGGCTTGCTTTGGTCGCGTCCCTTGCTCCCCTAGAGCGCCATCTTTTTCTGTATAA
  ACCTTGGCTCANGATCTGGNAGGCAAGCCGGTGGAAGTGGGGTGGGCCCACCTCTGCCCA
  TCCACTGACTGTGGGCCTGCCNTGGCTGGGCTGGCACGCCCAAACGTCGTNACCCCAGCA
  GTGCAGTCTGTTTTGCTCTTTTCTTCCCGCCACCTCC
  
• Restriction Sites: NotI-NotI
• ACCN: AK056253
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK056253.1
• RefSeq Size: 2365 bp
• RefSeq ORF: 2365 bp
• Locus ID: 5170
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Endometrial cancer, Focal adhesion, Insulin signaling pathway, mTOR signaling pathway, Non-small cell lung cancer, PPAR signaling pathway, Prostate cancer
• Gene Summary: Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.[UniProtKB/Swiss-Prot Function]