TIF1 gamma (TRIM33) (AK021491) Human Untagged Clone

CAT#: SC102040

(untagged)-Human cDNA FLJ11429 fis, clone HEMBA1001077, weakly similar to Human transcriptional intermediary factor 1 gamma mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIF1 gamma
• Synonyms: ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK021491, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTGAGCAGTCCTGAGAGTAGCTTGACACCACCTCTCTCAACCAACCTGCATCTAGAAAGTGAATTGG
  ATGCATTGGCAAGCCTGGAAAACCATGTGAAAACTGAACCTGCAGATATGAATGAAAGCTGCAAACAGTC
  AGGGCTCAGCAGCCTTGTTAATGGAAAGTCCCCAATTCGAAGCCTCATGCACAGGTCGGCAAGGATTGGA
  GGAGATGGCAACAATAAAGATGATGACCCAAATGAAGACTGGTGTGCTGTCTGCCAAAACGGAGGAGATC
  TCTTGTGCTGCGAAAAATGTCCAAAGGTCTTTCATCTAACTTGTCATGTTCCAACACTACTTAGCTTTCC
  AAGGTACCAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK021491 unedited
  AAAAGGGGTGGCCCATTAGGACTACTTATCATATAGGCGGCCGCCGATTCGGCACGAGGT
  CCAATTCTCACACACCTGTGAGACCCCCAATACTTCTAGTACTGGCAGTCGAGGCAGCTG
  TGGGTCATCAGGAAGAACTGCTGAGAAGACAAGTCTTAGTTTCAAATCTGATCAGGTGAA
  GGTCAAGCAAGAACCTGGGACTGAAGATGAAATATGTAGCTTTTCAGGAGGTGTAAAACA
  AGAAAAAACAGAGGATGGCAGGAGGAGTGCTTGCATGTTGAGCAGTCCTGAGAGTAGCTT
  GACACCACCTCTCTCAACCAACCTGCATCTAGAAAGTGAATTGGATGCATTGGCAAGCCT
  GGAAAACCATGTGAAAACTGAACCTGCAGATATGAATGAAAGCTGCAAACAGTCAGGGCT
  CAGCAGCCTTGTTAATGGAAAGTCCCCAATTCGAAGCCTCATGCACAGGTCGGCAAGGAT
  TGGAGGAGATGGCAACAATAAAGATGATGACCCAAATGAAGACTGGTGTGCTGTCTGCCA
  AAACGGAGGAGATCTCTTGTGCTGCGAAAAATGTCCAAAGGTCTTTCATCTAACTTGTCA
  TGTTCCAACACTACCTTAGCTTTTCAAGTGGGGGACTGGATATGCACATTTTGTAGAGAT
  ATTGGAAAGCCACAAGTTGAATTTGATTGTGATAATTTGCCACATAATAACAACGCGAAA
  ACTGCGCAGGGGTTTAGCCCCCTTGGACCAAAAGAAATGTGACCGTCTCCTGCTTTCCCT
  CTATTGCCATGAATCAAGTCTTGAATTCCCCGGAACCTGTTCCTGCTTTTATTCCAACCT
  CCTTTTAAATTCTCCACAAACCCCTTGGCTTCATCCCCCTCCGCCAAAAAACCTTCCCCA
  CN
  
• Restriction Sites: NotI-NotI
• ACCN: AK021491
• Insert Size: 1250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK021491.1, BAB13834.1
• RefSeq Size: 1460 bp
• RefSeq ORF: 363 bp
• Locus ID: 51592
• Cytogenetics: 1p13.2
• Domains: PHD
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transcription Factors
• Gene Summary: The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]