Nicastrin (NCSTN) (NM_015331) Human Untagged Clone

CAT#: SC100655

NCSTN (untagged)-Human nicastrin (NCSTN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Nicastrin
• Synonyms: ATAG1874
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100655 sequence for NM_015331 edited (data generated by NextGen Sequencing)
  ATGGCTACGGCAGGGGGTGGCTCTGGGGCTGACCCGGGAAGTCGGGGTCTCCTTCGCCTT
  CTGTCTTTCTGCGTCCTACTAGCAGGTTTGTGCAGGGGAAACTCAGTGGAGAGGAAGATA
  TATATCCCCTTAAATAAAACAGCTCCCTGTGTTCGCCTGCTCAACGCCACTCATCAGATT
  GGCTGCCAGTCTTCAATTAGTGGAGACACAGGGGTTATCCACGTAGTAGAGAAAGAGGAG
  GACCTACAGTGGGTATTGACTGATGGCCCCAACCCCCCTTACATGGTTCTGCTGGAGAGC
  AAGCATTTTACCAGGGATTTAATGGAGAAGCTGAAAGGGAGAACCAGCCGAATTGCTGGT
  CTTGCAGTGTCCTTGACCAAGCCCAGTCCTGCCTCAGGCTTCTCTCCTAGTGTACAGTGC
  CCAAATGATGGGTTTGGTGTTTACTCCAATTCCTATGGGCCAGAGTTTGCTCACTGCAGA
  GAAATACAGTGGAATTCGCTGGGCAATGGTTTGGCTTATGAAGACTTTAGTTTCCCCATC
  TTTCTTCTTGAAGATGAAAATGAAACCAAAGTCATCAAGCAGTGCTATCAAGATCACAAC
  CTGAGTCAGAATGGCTCAGCACCAACCTTCCCACTATGTGCCATGCAGCTCTTTTCACAC
  ATGCATGCTGTCATCAGCACTGCCACCTGCATGCGGCGCAGCTCCATCCAAAGCACCTTC
  AGCATCAACCCAGAAATCGTCTGTGACCCCCTGTCTGATTACAATGTGTGGAGCATGCTA
  AAGCCTATAAATACAACTGGGACATTAAAGCCTGACGACAGGGTTGTGGTTGCTGCCACC
  CGGCTGGATAGTCGTTCCTTTTTCTGGAATGTGGCCCCAGGGGCTGAAAGCGCAGTGGCT
  TCCTTTGTCACCCAGCTGGCTGCTGCTGAAGCTTTGCAAAAGGCACCTGATGTGACCACC
  CTGCCCCGCAATGTCATGTTTGTCTTCTTTCAAGGGGAAACTTTTGACTACATTGGCAGC
  TCGAGGATGGTCTACGATATGGAGAAGGGCAAGTTTCCCGTGCAGTTAGAGAATGTTGAC
  TCATTTGTGGAGCTGGGACAGGTGGCCTTAAGAACTTCATTAGAGCTTTGGATGCACACA
  GATCCTGTTTCTCAGAAAAATGAGTCTGTACGGAACCAGGTGGAGGATCTCCTGGCCACA
  TTGGAGAAGAGTGGTGCTGGTGTCCCTGCTGTCATCCTCAGGAGGCCAAATCAGTCCCAG
  CCTCTCCCACCATCTTCCCTGCAGCGATTTCTTCGAGCTCGAAACATCTCTGGCGTTGTT
  CTGGCTGACCACTCTGGTGCCTTCCATAACAAATATTACCAGAGTATTTACGACACTGCT
  GAGAACATTAATGTGAGCTATCCCGAATGGCTGAGCCCTGAAGAGGACCTGAACTTTGTA
  ACAGACACTGCCAAGGCCCTGGCAGATGTGGCCACGGTGCTGGGACGTGCTCTGTATGAG
  CTTGCAGGAGGAACCAACTTCAGCGACACAGTTCAGGCTGATCCCCAAACGGTTACCCGC
  CTGCTCTATGGGTTCCTGATTAAAGCCAACAACTCATGGTTCCAGTCTATCCTCAGGCAG
  GACCTAAGGTCCTACTTGGGTGACGGGCCTCTTCAACATTACATCGCTGTCTCCAGCCCC
  ACCAACACCACTTATGTTGTACAGTATGCCTTGGCAAATTTGACTGGCACAGTGGTCAAC
  CTCACCCGAGAGCAGTGCCAGGATCCAAGTAAAGTCCCAAGTGAAAACAAGGATCTGTAT
  GAGTACTCATGGGTCCAGGGCCCTTTGCATTCTAATGAGACGGACCGACTCCCCCGGTGT
  GTGCGTTCTACTGCACGATTAGCCAGGGCCTTGTCTCCTGCCTTTGAACTGAGTCAGTGG
  AGCTCTACTGAATACTCTACATGGACTGAGAGCCGCTGGAAAGATATCCGTGCCCGGATA
  TTTCTCATCGCCAGCAAAGAGCTTGAGTTGATCACCCTGACAGTGGGCTTCGGCATCCTC
  ATCTTCTCCCTCATCGTCACCTACTGCATCAATGCCAAAGCTGATGTCCTTTTCATTGCT
  CCCCGGGAGCCAGGAGCTGTGTCATACTGA
  
  Clone variation with respect to NM_015331.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015331 unedited
  GATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACCGCGCTAACAGACAG
  AGCCGAACGGGGGCTTCCGCTCAGCAGAGAGGCAAGATGGCTACGGCAGGGGGTGGCTCT
  GGGGCTGACCCGGGAAGTCGGGGTCTCCTTCGCCTTCTGTCTTTCTGCGTCCTACTAGCA
  GGTTTGTGCAGGGGAAACTCAGTGGAGAGGAAGATATATATCCCCTTAAATAAAACAGCT
  CCCTGTGTTCGCCTGCTCAACGCCACTCATCAGATTGGCTGCCAGTCTTCAATTAGTGGA
  GACACAGGGGTTATCCACGTAGTAGAGAAAGAGGAGGACCTACAGTGGGTATTGACTGAT
  GGCCCCAACCCCCCTTACATGGTTCTGCTGGAGAGCAAGCATTTTACCAGGGATTTAATG
  GAGAAGCTGAAAGGGAGAACCAGCCGAATTGCTGGTCTTGCAGTGTCCTTGACCAAGCCC
  AGTCCTGCCTCAGGCTTCTCTCCTAGTGTACAGTGCCCAAATGATGGGTTTGGTGTTTAC
  TCCAATTCCTATGGGCCAGAGTTTGCTCACTGCAGAGAAATACAGTGGAATTCGCTGGGC
  AATGGTTTGGCTTATGAAGACTTTAGTTTCCCCATCTTTCTTCTTGAAGATGAAAATGAA
  ACCAAAGTCATCAAGCAGTGCTATCAAGATCACAACCTGAGTCAGAATGGCTCAGCACCA
  ACCTTCCCACTATGTGCCATGCAGCTCTTTTCACACATGCATGCTGTCATCAGCACTGCC
  ACCTGCATGCNGCGCAGCTCCATCCAAAGCACCTTCAGCATCAACCCAGAAATCGTCTGT
  GACCCCCTGTCTGATTACCATGTGTGGAGCATGCTAAAGCCTATNAATACCACTGGGACA
  TTAAAGCCTGACGACAGGGTTGTGGNTTGCTGCACCNNGCTGATAAGTCGTCCTTTTTCT
  GGATGTGGCCCCAGGGGCTGAAGCGCATGGCTTCTTTGTACCCACTGGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015331 unedited
  GGGGGGTGAAAGTCAATGCTTTTTGATGNCAACTCTTAGTATATATTTACAAAAGCAATA
  AGGAAAAAGACAAAAACAAAAATAGATATTACAGCTTAATAAAAAGTACAACTGAGCACT
  GTGGGCTGGAGGTGGGATACCCACCCGCAGCACGCCCACCCTAATGTGCCATCTGAGGGC
  CTGCCACAAAGGACAGTGGGCAACGAGACAGTAGGCTCAGCCCATATATTCCTCTGTCCT
  CAGGGGCTCAATGTACCTTGACCCTGACATTAAACCTTTTATGTACCTTCCCAGTCACGA
  AGGTGGCTTGGGTACATTGGATGCGGGCGCGCGCGATCATTATAGAGGTACAGGGCAGGA
  AGGAAAAAGTAGAAGGGTCCTGAAGGGCCAATTATGGTGGGTAACTAGGGAGTTAAACAG
  GAGCAAGAAGCTTCTATTTGTAATCCCGCCCTCTGGCATGACGCCGAGAAGGAAGAGGAA
  ATGGGGAAGGGGTGATGGGAAAGGGGAGCGGATTGAAGTATAATCGGTGACTTTCTCTAC
  ACCGATTTTCGGAATTTGCCATATTTTTTTTCCCCCGCTATTTTTTCCGCCCCCCTCCAT
  CCCCCGAAATTAATGTTATGGGTTTTCACTTTACGGCGCCCACCTCTCCATTCATCCCCC
  CTGATTGTTTCTCCCCATGAGCCCCCCTTTTGTTTGGCGCCCAATTGGACCAGCGGTATT
  AGTTCCCACTGATTTGTATTTGTGCATAAAACGAGAGGATTGCGCCGTTTTTATGTTGTC
  CCGACTGTTGGGTCCTGCCTGACTCTAATCGGCTGTAATGGGCCGTATAGAATTACGTTT
  AGGCCCGGTCGCCTGTTTTTTCTCCCGTTTGTGTCGCCCCCCCCCCCCCGTTTTTTGTTT
  TATTTN
  
• Restriction Sites: Please inquire
• ACCN: NM_015331
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015331.2, NP_056146.1
• RefSeq Size: 2944 bp
• RefSeq ORF: 2130 bp
• Locus ID: 23385
• UniProt ID: Q92542
• Cytogenetics: 1q23.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease, Notch signaling pathway
• Gene Summary: This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) encodes the longest isoform (1).