PI 3 Kinase regulatory subunit 4 (PIK3R4) (NM_014602) Human Untagged Clone

CAT#: SC100382

PIK3R4 (untagged)-Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PI 3 Kinase regulatory subunit 4
• Synonyms: p150; VPS15
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014602 edited
  CGCGAATTCGGCACGAGGGTTGCTTTCCCGGGAGTTCGGCGTTTGCTGGGGCTGCAGCAG
  CTGAAGTGTAGTGTTTTCTTGGGACTGGCGGTCTGCACTTCTCTCCCGGGTTCCATCTCC
  CCCCGCCCGGTGGTGAGGCCCTCGAGGAGGGCTCGGACGGGTGTAGCGATCCGCGCTAGA
  GGAAGACGAGGCCCGGGAACGCATGTCCCCCAGGGCAGGTTAGGGGGCTGGAGGGGTCAA
  ATCCCGGGGTACTTGTGGAGACTCTTTAGCGTGGCTTCTTCTCTCTGCTGAGACCCCGAG
  AGCTTTCCCAGTTCTCCTCCCAGGACCACCGGGGTTCCTGAAGATCGGGACTTTTCTGCG
  CCCCTCCACCAACAGCCCATCTCCTGTCTATGAAGAAAGACCCTTCGTAGAAACAACTTC
  CCCGCTGCTGACGCGTTTTCCCGTCCCGTCCCCGAAGTAGTCTACTATGACCTCGTTGTG
  AGCCTCTGAACGATTTTGACACTTTCCCGAGGCCTAGGGTATTATATCCTAACCTTACTA
  AAGACCACAGAGGTGCTTGCCATTATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAG
  ATCCTTTCTGTAGAGAGTTATTTTTCAGATATTCATGACTTTGAATATGATAAAAGCCTG
  GGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGAGAAGGCCTGGTCGTTGTG
  AAGGTTTTTGCAATTCAGGATCCCACATTGCCTTTAACCAGCTATAAACAAGAGCTGGAG
  GAACTGAAAATCAGGCTTAATTCTGCACAGAATTGTCTACCTTTCCAGAAAGCATCAGAA
  AAAGCATCTGAGAAAGCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGAT
  CGCATCAGTACCCGTCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTTTCCAGATC
  CTGACAGCTGTGGACCAAGCACACAAATCTGGAGTTCGTCATGGGGACATCAAGACTGAG
  AATGTGATGGTCACCAGTTGGAATTGGGTTCTTCTAACTGATTTTGCCAGTTTTAAGCCC
  ACTTATCTTCCAGAAGACAACCCGGCAGATTTCAATTATTTCTTTGACACATCACGGAGG
  AGAACTTGCTATATTGCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTA
  GAATATATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGAGAACAAGA
  GGAGAGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGTGTGATAGCTGAGCTTTTT
  ACAGAAGGTGTACCATTATTTGATCTCTCTCAACTTTTGGCTTATAGAAATGGACATTTT
  TTCCCTGAACAAGTGCTAAATAAAATTGAAGATCACAGTATCAGAGAATTGGTAACTCAG
  ATGATTCACCGTGAGCCAGATAAACGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGT
  GGCAATGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTACATGGCCCAGTTTGCC
  AAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATACGGAAGGATTTGGGCAAC
  ATTATTCACAATCTCTGTGGACATGATCTGCCAGAAAAAGCCGAAGGAGAGCCTAAGGAA
  AATGGGCTGGTTATCTTGGTATCTGTTATAACATCCTGCCTACAGACCCTTAAATACTGT
  GATTCCAAACTAGCTGCTTTGGAACTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAA
  ATCCTTTTGGATCGTATTACTCCATATCTTTTGCATTTCAGCAATGACTCTGTTCCTAGG
  GTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAGTTCTTGCTCTCGTCAAAGAGGTTCCT
  CGTAATGATATCAATATTTATCCGGAATACATTCTGCCAGGCATAGCCCACTTAGCCCAA
  GATGATGCTACTATCGTTAGACTAGCCTATGCTGAAAACATAGCTCTGCTGGCAGAAACA
  GCTCTGAGATTCCTGGAATTAGTACAGTTAAAAAATCTTAATATGGAAAATGACCCCAAT
  AATGAAGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGCTCCAAGCC
  TTACATGAAATGGTCCAGCAGAAAGTTGTTACTTTGCTAAGTGACCCTGAAAATATTGTA
  AAACAAACCTTGATGGAAAATGGAATAACACGGCTGTGTGTATTCTTTGGACGTCAGAAA
  GCCAACGATGTTTTGTTGTCCCACATGATTACTTTCCTAAATGATAAGAATGATTGGCAT
  CTACGTGGAGCATTTTTTGATAGTATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGC
  TCCTCAATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAATTTGTCATT
  GTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGACTGCTACAAAAACCCCAT
  GTTTACGAATTTGCCAGTGATATTGCCCCCTTCCTGTGTCATCCCAATTTATGGATACGT
  TATGGTGCCGTGGGATTTATCACAGTGGTAGCTCGTCAAATAAGTACAGCTGATGTCTAC
  TGTAAACTGATGCCTTATCTTGACCCATATATTACCCAACCAATAATACAGATTGAAAGA
  AAACTTGTTCTGCTCAGTGTTTTAAAGGAACCAGTAAGTCGTTCTATATTTGATTATGCT
  TTGAGGTCTAAAGATATTACTAGCTTGTTCAGACATCTTCACATGCGTCAGAAGAAACGA
  AATGGTTCTCTTCCCGACTGCCCTCCGCCAGAGGATCCTGCCATAGCACAGCTTCTGAAG
  AAGTTGCTCTCACAGGGAATGACAGAGGAAGAGGAAGACAAACTTCTGGCACTGAAAGAC
  TTCATGATGAAATCTAATAAAGCAAAGGCCAATATAGTGGACCAGAGCCATCTTCATGAT
  AGTAGTCAGAAAGGTGTAATTGACTTGGCAGCTTTAGGCATAACTGGGAGACAAGTTGAT
  CTTGTTAAAACCAAACAAGAACCAGATGACAAACGGGCCAGAAAACATGTAAAACAAGAC
  TCAAATGTAAATGAAGAATGGAAAAGCATGTTTGGGTCACTGGACCCACCAAACATGCCA
  CAGGCCCTACCTAAAGGGAGTGATCAGGAGGTGATTCAGACTGGGAAACCTCCTCGTTCC
  GAGTCCTCTGCTGGCATTTGTGTCCCTTTGTCAACTTCTTCACAGGTTCCAGAAGTGACA
  ACTGTCCAAAATAAAAAACCAGTAATACCGGTTTTAAGTAGTACAATCTTACCATCCACC
  TATCAGATTCGAATTACAACTTGTAAAACTGAACTTCAGCAACTCATCCAGCAAAAGCGG
  GAGCAGTGCAATGCTGAGAGAATAGCTAAGCAGATGATGGAAAATGCTGAATGGGAGAGT
  AAACCACCACCACCTGGATGGCGTCCTAAAGGGCTGTTAGTTGCCCATCTTCATGAGCAT
  AAATCTGCTGTGAATCGAATTAGAGTCTCTGATGAACACTCACTTTTTGCAACATGTTCA
  AATGATGGCACAGTGAAAATCTGGAACAGTCAAAAGATGGAGGGGAAGACCACCACTACC
  AGATCTATTCTTACATACAGCCGAATTGGAGGACGAGTCAAGACGCTCACATTCTGCCAA
  GGCTCCCACTATTTAGCCATAGCATCTGATAATGGTGCTGTCCAGCTTCTTGGAATTGAG
  GCTTCTAAGCTGCCCAAGTCTCCTAAAATCCATCCTCTACAAAGCAGAATTCTAGATCAG
  AAGGAGGACGGTTGTGTTGTGGATATGCATCACTTCAACTCTGGAGCACAGTCTGTTCTT
  GCCTATGCCACTGTGAATGGCTCTCTGGTTGGCTGGGACCTTAGGTCTTCAAGCAATGCG
  TGGACTTTAAAGCATGATTTAAAGTCGGGCCTCATCACTTCCTTTGCTGTGGACATCCAC
  CAATGCTGGCTCTGCATTGGTACAAGCAGTGGTACCATGGCTTGTTGGGACATGAGGTTC
  CAGTTGCCAATTTCAAGTCACTGTCATCCTTCCAGGGCTCGAATCAGACGCCTCTCAATG
  CACCCTCTGTATCAGTCCTGGGTGATTGCAGCTGTTCAGGGCAACAACGAAGTGTCCATG
  TGGGACATGGAGACTGGTGACAGAAGATTTACTCTCTGGGCCAGCAGTGCACCACCACTT
  TCTGAATTACAGCCTTCTCCTCATAGCGTCCATGGTATCTACTGTAGTCCTGCAGATGGA
  AATCCTATCCTACTAACAGCTGGCTCAGATATGAAAATAAGGTTTTGGGACTTGGCTTAC
  CCAGAAAGGTCCTATGTTGTTGCAGGAAGTACTAGTTCCCCATCTGTGTCCTACTACAGG
  AAAATAATTGAAGGCACTGAAGTTGTCCAGGAAATTCAGAATAAGCAGAAAGTAGGACCA
  AGTGATGACACCCCTCGAAGGGGCCCAGAGTCCCTGCCCGTGGGACATCATGACATCATC
  ACTGATGTCGCCACATTCCAGACCACACAGGGCTTCATCGTAACTGCTTCTAGAGATGGG
  ATTGTGAAGGTGTGGAAATAAAACCTACTGATTTGTATAAATTTTAATAGTTATAAATAT
  AATACTATAACTCGAGAAAAGGCATTTCTAGAGAACAGATTCATTTGCTTAATTTTCAAA
  ATTATGTCTCCATATTACTGTTTCATGACTGACTGACTAAATGACACCCAAAATGGTTAA
  GATGTACTTGACTAGTTTACTTATGCATCTCTTTGCAAGAATCAGCCAGCCAACAATGTC
  TGGGATTTTTATTGTATATGTTATAGAGGTGAGAAATGTAAAATATGAAAATGAATATGT
  TTATTTTGTATTGAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014602 unedited
  TGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGCTTTCCCGGGAG
  TTCGGCGTTTGCTGGGGCTGCAGCAGCTGAAGTGTAGTGTTTTCTTGGGACTGGCGGTCT
  GCACTTCTCTCCCGGGTTCCATCTCCCCCCGCCCGGTGGTGAGGCCCTCGAGGAGGGCTC
  GGACGGGTGTAGCGATCCGCGCTAGAGGAAGACGAGGCCCGGGAACGCATGTCCCCCAGG
  GCAGGTTAGGGGGCTGGAGGGGTCAAATCCCGGGGTACTTGTGGAGACTCTTTAGCGTGG
  CTTCTTCTCTCTGCTGAGACCCCGAGAGCTTTCCCAGTTCTCCTCCCAGGACCACCGGGG
  TTCCTGAAGATCGGGACTTTTCTGCGCCCCTCCACCAACAGCCCATCTCCTGTCTATGAA
  GAAAGACCCTTCGTAGAAACAACTTCCCCGCTGCTGACGCGTTTTCCCGTCCCGTCCCCG
  AAGTAGTCTACTATGACCTCGTTGTGAGCCTCTGAACGATTTTGACACTTTCCCGAGGCC
  TANGGTATTATATCCTAACCTTACTAAAGACCACAGAGGTGCTTGCCATTATGGGAAATC
  AGCTTGCTGGCATTGCTCCCTCCCAAATCCTTTCTGTAGAGAGTTATTCCTCCGATATTC
  ATGACTTTGAAAATGATAAAAACCCGCGGAGTACCTCCGGTTTTTAAAGCTGCTCGACCC
  AATCACCGAAAGGCCCTGGCCGTTGTGAAAGTTTTTGAATTCAAGAACCCCAATTTGCCT
  TCACCCCCTTTCAACAAAAAGCTGGAGGGAACCGCAAAATAAGGCCTCAATTCTGCCCCC
  ACTCGCTCACTCTTTCCAAAATACTCCCAAAAACGCCCCCCCGAAATCCTCTCCCTCCTT
  TTAGCCCTTTGTGCCATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014602 unedited
  TGGGTTTTCTATGNACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTACAG
  TTTAACTGCTTTGAATAGCATTTTTAATAGCAAAAATGTGTTTATACTATAATAGCTTAC
  AACCATCTTTTCAACCATCTTTTTCAATACAAAATAAACATATTCATTTTCATATTTTAC
  ATTTCTCACCTCTATAACATATACAATAAAAATCCCAGACATTGTTGGCTGGCTGATTCT
  TGCAAAGAGATGCATAAGTAAACTAGTCAAGTACATCTTAACCATTTTGGGTGTCATTTA
  GTCAGTCAGTCATGAAACAGTAATATGGAGACATAATTTTGAAAATTAAGCAAATGAATC
  TGTTCTCTAGAAATGCCTTTTCTCGAGTTATAGTATTATATTTATAACTATTAAAATTTA
  TACAAATCAGTAGGTTTTATTTCCACACCTTCACAATCCCATCTCTAGAAGCAGTTACGA
  TGAAGCCCTGTGTGGTCTGGAATGTGGCGACATCAGTGATGATGTCATGATGTCCCACGG
  GCAGGGACTCTGGGCCCCTTCGAGGGGTGTCATCACTTGGTCCTACTTTCTGCTTATTCT
  GAATTTCCTGGACAACTTCAGTGCCTTCAATTATTTTCCTGTAGTAGGACACAGATGGGG
  AACTAGTACTTCCTGCAACAACATAGGACCTTTCTGGGTAAGCCAAGTCCCAAAACCTTA
  TTTTCATATCTGAGCCAGCTGTTAGTAGGATAGGATTTCCATCTGCAGGACTACAGTAGA
  TACCATGGACGCTATGAGGAGAAGGCTGTAATTCAGAAAATGGTGGTGCACTGCTGGCCC
  AGAGAGTAAATCTTCTGTCACCAGTCTCCATGTCCCACATGGACACTTCGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014602
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014602.1, NP_055417.1
• RefSeq Size: 5060 bp
• RefSeq ORF: 4077 bp
• Locus ID: 30849
• UniProt ID: Q99570
• Cytogenetics: 3q22.1
• Domains: pkinase, WD40, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Regulation of autophagy
• Gene Summary: Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).[UniProtKB/Swiss-Prot Function]