Adgrl2 (NM_001302209) Rat Untagged Clone

CAT#: RN217633

Lphn2 (untagged) - Rat latrophilin 2 (Lphn2), transcript variant 4

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Adgrl2
• Synonyms: Cirl-2; Cirl2; Cl2ac; Lphn2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217633 representing NM_001302209
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCTTCTGGTTGCAGAATGCGAAGTCTCTGGTTTATCATGATAATCAGTTTCTCACCGAATACCG
  AAGGTTTCAGCAGAGCAGCCTTGCCATTCGGGTTAGTTAGACGAGAGCTGTCCTGTGAAGGTTATTCTAT
  AGACCTGCGATGTCCGGGCAGTGACGTCATCATGATCGAGAGCGCAAACTACGGTCGGACGGACGACAAG
  ATCTGCGACGCAGACCCCTTTCAGATGGAGAACACAGACTGCTACCTCCCTGATGCCTTCAAAATCATGA
  CTCAAAGGTGCAACAACCGAACACAGTGTGTAGTAGTTACCGGGTCAGATGTATTTCCTGATCCATGTCC
  TGGAACTTACAAATACCTTGAAGTTCAATATGAATGTGTCCCTTACATGGAGCAAAAAGTTTTTGTGTGT
  CCTGGAACCTTGAAAGCAATTGTGGACTCTCCAAGTATCTATGAAGCTGAGCAAAAGGCAGGTGCTTGGT
  GCAAGGACCCCCTTCAGGCTGCAGATAAAATTTATTTTATGCCCTGGACTCCCTACCGCACCGATACCTT
  AATAGAATATGCTTCTTTAGAAGATTTTCAAAACAGCCGCCAGACAACAACATACAAACTTCCAAACCGA
  GTGGACGGTACTGGATTTGTGGTGTATGACGGGGCAGTCTTCTTCAACAAAGAAAGAACGAGAAACATTG
  TTAAATTTGACTTGAGGACTAGAATCAAGAGTGGGGAGGCCATAATCAACTACGCCAACTACCATGACAC
  TTCACCCTACAGATGGGGGGGGAAGACTGACATTGACCTGGCAGTGGATGAAAATGGCTTGTGGGTCATC
  TACGCCACCGAGCAGAACAACGGAATGATCGTGATTAGCCAGCTCAATCCGTACACTCTCCGATTCGAAG
  CAACCTGGGAGACGACGTATGACAAGCGTGCGGCGTCCAATGCTTTCATGATATGCGGGGTCCTCTACGT
  GGTCAGGTCAGTGTACCAAGACAATGAAAGCGAAGCTGGCAAGAACGTCATCGACTACATTTACAACACA
  AGGTTGAGCCGGGGAGAGCACGTGGACGTTCCCTTCCCCAACCAGTACCAGTACATCGCTGCAGTGGATT
  ACAACCCAAGAGACAACCAACTCTACGTATGGAACAATAACTTTATCTTACGGTATTCTCTGGAGTTTGG
  TCCACCCGACCCTGCCCAAGTGCCTACCACAGCTGTGACAATAACTTCTTCAGCTGAGCTGTTCAAAACC
  ACAGTGTCAACCACAAGCAGTACTTCACAGAGAGGCCCCGTGAGCAGCACAGTCGCTGGTCCTCAGGAAG
  GAAGCCGAGGGACAAAGCCACCTCCAGCAGTCTCTACAACCAAAATTCCTCCTGTAACAAATATTTTTCC
  CCTGCCAGAGAGATTCTGCGAAGCGTTAGAAATGAAGGGGATAAAGTGGCCTCAGACACAAAGGGGGATG
  ATGGTTGAGCGACCGTGTCCCAAGGGAACAAGAGGAACGGCCTCGTATCTCTGCATGGCTTCCACAGGAA
  CCTGGAACCCGAAGGGCCCGGATCTTAGCAACTGCACCTCTCACTGGGTGAATCAGCTGGCCCAGAAGAT
  CAGAAGTGGAGAGAATGCTGCAAGTCTGGCCAACGAACTGGCTAAGCACACCAAGGGGACGGTGTTCGCT
  GGGGATGTGAGCTCCTCTGTGAGGCTGATGGAACAGTTGGTGGACATCCTGGATGCCCAGCTGCAGGAGC
  TGAAACCGAGCGAGAAGGACTCGGCCGGGAGGAGTTATAACAAGCTCCAAAAACGAGAGAAGACATGCAG
  GGCTTACCTTAAGGCCATTGTGGACACAGTAGATAACCTTCTGAGAGCCGAGACTTTGGACTGCTGGAAA
  CACATGAATTCCTCAGAGCAGGCGCACACAGCCACCATGCTGTTGGACACCTTGGAAGAAGGAGCATTTG
  TCCTGGCAGACAACCTTTTGGAACCAACCCGGGTCTCAATGCCAACGGATAATATTGTTCTAGAAGTCGC
  TGTCCTCAGCACGGAAGGACAGGTCCAAGACTTCACCTTCCATCTCGGCTTCAAGGGGGCCTTCAGCTCC
  ATCCAGCTCTCAGCCAACACCGTCAAGCAAAACAGCAGAAACGGGCTGGCAAAGGTGGTATTCATCATTT
  ACCGGAGTCTGGGACCATTCCTGAGCACCGAAAATGCGACCGTCAAACTGGGCGCAGACCTCCTGGGTCG
  GAACAGCACCATCGCAGTGAACTCGCACGTCCTTTCAGTCTCCATCAATAAGGAGTCCAGCCGTGTGTAC
  TTGACAGACCCGGTGCTTTTTTCAATGCCACACATTGATTCTGACAATTATTTCAACGCAAACTGCTCCT
  TCTGGAACTACTCAGAGAGAACCATGATGGGATATTGGTCTACCCAGGGCTGCAAGCTGGTTGACACTAA
  TAAAACTCGCACGACGTGTGCATGCAGCCACCTAACCAATTTTGCTATTCTCATGGCCCACAGGGAAATT
  GTGTACAAAGATGGCGTCCACAAATTGCTGCTGACAGTCATCACCTGGGTGGGCATCGTTGTCTCCCTCG
  TCTGCCTGGCTATCTGCATCTTCACCTTCTGCTTCTTCCGAGGCCTGCAAAGCGACCGCAACACGATCCA
  CAAGAACCTGTGTATCAACCTCTTCATCGCTGAGTTTATTTTCCTAATAGGCATTGATAAAACACAGTAC
  ACGATTGCGTGCCCCGTGTTTGCAGGACTCCTGCACTTTTTCTTCCTGGCTGCTTTTTCCTGGATGTGCC
  TAGAAGGTGTGCAGCTCTACCTCATGTTGGTTGAAGTTTTCGAGAGTGAATACTCAAGGAAGAAGTATTA
  CTATGTCGCCGGGTACCTCTTCCCTGCCACAGTGGTCGGTGTTTCAGCTGCTATCGACTACAAGAGTTAC
  GGGACACTAGAGGCTTGCTGGCTTCACGTTGATAACTATTTCATATGGAGTTTCATTGGGCCTGTTACTT
  TCATCATTCTGCTAAATATTATTTTCCTGGTGATCACGCTGTGCAAAATGGTGAAACATTCAAACACTTT
  GAAACCAGATTCTAGCAGGTTGGAAAACATTAAGTCTTGGGTGCTCGGTGCGTTCGCCCTGCTGTGTCTC
  CTGGGCCTAACCTGGTCCTTTGGGTTGCTTTTTGTTAACGAGGAGACCGTTGTCATGGCTTATCTCTTCA
  CCGCCTTTAATGCTTTCCAGGGACTGTTTATTTTCATCTTCCACTGTGCTCTTCAAAAGAAAGTACGGAA
  AGAGTATGCCAAGTGCTTCAGACACTGGTACTGCTGTGGTGGCCTCCCGACCGAGAGCCCGCACAGCTCT
  GTAAAGGCGTCCACCTCCCGCACCAGTGCTCGCTACTCCTCTGGTACACAGAGCCGTATAAGAAGGATGT
  GGAATGACACCGTGAGGAAGCAGTCTGAATCGTCTTTTATCTCAGGTGACATCAATAGCACTTCTACCCT
  TAATCAAGGAATGACTGGCAATTACCTACTAACAAACCCTCTTCTTCGACCCCACGGCACTAACAACCCC
  TATAACACATTGCTCGCTGAAACAGTTGTATGTAATGCCCCTTCAGCGCCCGTGTTTAACTCACCAGGAC
  ATTCACTGAACAATACCCGGGACACCAGCGCCATGGATACTCTACCGCTAAATGGTAACTTCAACAACAG
  CTACTCCCTGCGCAAGGCCGACTACCACGACGGCGTGCAGGTTGTGGACTGTGGACTAAGTCTGAACGAC
  ACCGCGTTTGAGAAAATGATCATTTCAGAGTTAGTGCACAACAACCTCCGGGGTAGCAACAAAACCCACA
  ACTTGGAGCTCAAGCTCCCGGTTAAACCCGTGATTGGCGGCAGCAGCAGCGAAGATGACGCGATCGTGGC
  CGACGCCTCATCTTTGATGCACGGTGATAACCCAGGGCTGGAATTCCGCCACAAAGAGCTGGAGGCACCG
  CTCATCCCTCAGCGGACTCACTCGCTTCTGTACCAACCCCAGAAAAAAGTGAAACCCGAGGCAACCGACA
  GCTACGTCTCCCAGCTGACGGCCGAGGCCGACGAGCACCTCCAGTCCCCCAACAGAGACTCTCTGTACAC
  GAGCATGCCCAACCTAAGAGACTCTCCCTACCCGGAGAGCAGCCCGGACATGGCAGAGGACCTGTCTCCC
  TCCAGGAGGAGCGAGAACGAGGACATTTACTACAAAAGTATGCCCAATCTTGGGGCTGGCCGCCAGCTCC
  AGATGTGCTACCAGATCAGCAGAGGCAATAGCGATGGCTACATCATCCCCATTAACAAAGAAGGGTGCAT
  CCCAGAGGGGGACGTCAGGGAAGGACAGATGCAGCTGGTAACAAGTCTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302209
• Insert Size: 4392 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302209.1, NP_001289138.1
• RefSeq Size: 6210 bp
• RefSeq ORF: 4392 bp
• Locus ID: 171447
• UniProt ID: O88923
• Cytogenetics: 2q45
• Gene Summary: low affinity G-protein-coupled receptor for alpha-latrotoxin [RGD, Feb 2006]
  Transcript Variant: This variant (4) lacks two consecutive exons in the 3' coding region, but maintains the reading frame, compared to variant 1. The resulting isoform (4) lacks an internal segment, compared to isoform 1.