Actn2 (NM_001170325) Rat Untagged Clone

CAT#: RN217307

Actn2 (untagged) - Rat actinin alpha 2 (Actn2)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Actn2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217307 representing NM_001170325
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCAGATAGAGCCCGGCGTGCAGTACAACTATGTCTACGACGAAGATGAGTACATGATCCAGGAGG
  AGGAGTGGGACCGCGACCTGCTCCTCGACCCGGCCTGGGAGAAGCAGCAGAGGAAGACTTTCACTGCCTG
  GTGCAACTCGCACCTGCGCAAAGCCGGCACCCAGATCGAGAACATAGAGGAGGATTTCAGGAATGGCCTC
  AAGCTCATGCTGCTTCTGGAAGTCATCTCAGGGGAAAGGCTTCCCAAACCTGACCGGGGCAAAATGCGGT
  TCCACAAGATTGCGAATGTCAACAAGGCTTTGGATTACATAGCCAGCAAGGGAGTGAAATTGGTATCTAT
  TGGGGCTGAAGAAATCGTCGATGGCAATGTGAAGATGACCCTGGGTATGATCTGGACCATCATCCTCCGC
  TTCGCCATTCAGGACATCTCAGTGGAAGAAACATCTGCCAAAGAGGGCCTGCTGCTATGGTGTCAGAGGA
  AGACAGCACCATACCGAAATGTGAACATCCAGAACTTCCACACCAGCTGGAAAGATGGCCTTGGACTCTG
  TGCCCTCATCCACAGACACCGGCCTGACCTCATCGACTACTCAAAGCTTAACAAGGATGACCCCATAGGA
  AACATTAACCTGGCCATGGAAATAGCAGAGAAGCACTTGGATATCCCTAAAATGTTGGATGCTGAAGACA
  TTGTCAACACTCCCAAACCCGATGAAAGAGCCATCATGACTTACGTTTCCTGCTTCTACCATGCTTTCGC
  GGGAGCGGAGCAGGCCGAGACGGCAGCTAACAGGATATGTAAGGTTCTTGCTGTGAATCAAGAGAATGAG
  AGGCTGATGGAAGAATATGAGAGGCTAGCGAGTGAGCTCTTGGAATGGATTCGACGCACAATCCCCTGGC
  TGGAGAACAGGACACCGGAGAAGACCATGCAAGCCATGCAGAAGAAGCTGGAGGACTTCCGGGACTATCG
  TCGTAAGCACAAGCCCCCAAAGGTGCAGGAGAAGTGCCAGCTGGAGATCAACTTCAACACCCTGCAGACC
  AAGCTTCGGATCAGCAACCGGCCTGCCTTCATGCCCTCCGAGGGCAAGATGGTGTCGGACATTGCTGGTG
  CCTGGCAGAGGTTGGAGCAGGCCGAGAAGGGCTATGAGGAATGGCTATTGAATGAGATTCGAAGACTGGA
  GCGCTTGGAGCACTTGGCTGAGAAGTTCAGGCAGAAGGCCTCCACCCATGAGACATGGGCTTACGGCAAA
  GAACAGATCTTGCTGCAGAAGGACTATGAGTCTGCGTCCCTGACGGAAGTTCGAGCCCTACTTCGGAAGC
  ACGAGGCCTTTGAGAGTGACCTGGCGGCACACCAGGATCGTGTGGAACAGATTGCAGCCATTGCTCAGGA
  GCTCAATGAACTGGACTACCACGATGCTGTGAACGTCAATGATCGTTGCCAGAAAATTTGCGACCAGTGG
  GATAGATTGGGGACGCTCACCCAGAAGAGGAGGGAGGCCTTGGAGAGAACCGAGAAGTTGCTAGAAACAA
  TTGACCAGCTGCACCTGGAGTTTGCCAAGAGAGCTGCGCCCTTCAACAACTGGATGGAGGGCGCCATGGA
  GGACCTGCAGGACATGTTCATCGTCCACAGCATTGAGGAGATCCAGAGTCTGATCACCGCACATGAGCAG
  TTCAAGGCCACGCTGCCCGAGGCCGATGGGGAGCGGCAGTCCATCCTAGCCATCCAGAATGAGGTGGAGA
  AGGTGATCCAGAGCTACAGTATCCGGATCAGCTCCAGCAACCCCTACAGCACTGTCACCATGGACGAGCT
  GCGCAACAAGTGGGACAAGGTGAAGCAGCTGGTGCCAGTCCGGGATCAGTCCCTGCAGGAGGAGCTGGCA
  CGCCAACATGCTAATGAGCGCCTACGGCGCCAGTTTGCTGCCCAGGCCAATGCCATCGGCCCCTGGATCC
  AGAACAAGATGGAGGAGATCGCTCGGAGCTCCATCCAGATCACAGGGGCCCTGGAGGACCAGATGAACCA
  GCTGAAGCAGTACGAGCACAACATCATCAACTACAAGAACAACATTGACAAGCTGGAGGGCGACCACCAG
  CTCATCCAGGAAGCCCTGGTCTTCGACAACAAGCACACCAACTACACCATGGAGCATATCCGTGTCGGAT
  GGGAGTTGCTGCTCACAACTATCGCCAGAACCATCAATGAGGTGGAGACCCAGATCCTGACAAGAGATGC
  CAAGGGCATCACCCAGGAGCAGATGAATGAGTTCAGAGCCTCCTTCAACCACTTTGACAGGAGGAAGAAT
  GGCCTGATGGATCATGAGGATTTCAGAGCCTGCCTCATTTCCATGGGCTATGACTTGGGTGAAGCTGAGT
  TTGCCCGAATTATGACTCTGGTTGACCCCAACGGACAAGGCACGGTCACCTTCCAGTCCTTCATTGACTT
  CATGACTAGAGAGACCGCAGACACGGACACTGCAGAACAGGTCATCGCCTCCTTCCGGATTCTGGCTTCT
  GATAAGCCTTACATCTTGGCAGAGGAACTTCGTCGAGAGCTGCCTCCGGATCAGGCCCAGTACTGCATCA
  AGAGAATGCCCCCATACTCAGGCCCAGGCAGTGTCCCCGGGGCCCTGGACTACACTGCCTTCTCCTCTGC
  CCTCTACGGGGAGAGCGACCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001170325
• Insert Size: 2685 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170325.1, NP_001163796.1
• RefSeq Size: 2943 bp
• RefSeq ORF: 2685 bp
• Locus ID: 291245
• Cytogenetics: 17q12.1