Mx1 (NM_001271060) Rat Untagged Clone

CAT#: RN217053

Mx1 (untagged) - Rat myxovirus (influenza virus) resistance 1 (Mx1), transcript variant 4

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mx1
• Synonyms: IFI78
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217053 representing NM_001271060
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGCAGACCTCTGCATGCCGTCACCAGACACCACAGAAACATCCTGATACCTCTGAAGAGAGCC
  AGGCAATGGAGTCTGTGGATAACCTCTGCAGTCAGTATGAGGAGAAGGTGCGGCCCTGCATTGACCTCAT
  CGACTCCCTGCGAGCTCTGGGTGTGGAGCAAGACCTGGCCCTGCCTGCCATCGCTGTCATCGGGGACCAG
  AGTTCAGGGAAGAGCTCTGTGCTGGAAGCACTGTCTGGAGTGGCCCTCCCCAGAGGCAGTGGTATTGTTA
  CCAGGTGTCCTCTGGTGCTGAAATTGAAGCAACTGAAGCAGGGAGAAAAATGGAGCGGCAAGGTCATTTA
  TAAGGACACCGAGATTGAGATCTCACACCCTTCACTGGTAGAAAGGGAAATCAATAAAGCCCAGAACTTG
  ATTGCTGGGGAAGGGTTGAAGATTAGCTCTGATCTCATTAGCTTGGAGGTTAGCTCTCCACATGTCCCAG
  ACCTGACTCTGATTGACCTTCCTGGTATCACAAGAGTGGCTGTGGGTGACCAGCCTGCAGACATCGAACA
  CAAGATCAAGAGACTTATCACTGAATACATCCAGAAACAGGAGACCATCAACCTGGTGGTGGTCCCCAGC
  AATGTGGACATTGCCACCACAGAGGCCCTGAAAATGGCTCAGGAGGTGGACCCTCAAGGGGATAGAACCA
  TAGGGATCTTGACCAAGCCCGATCTGGTGGACAGAGGGACTGAAGACAAGGTCGTGGATGTGGTGCGGAA
  CCTCGTGTGCCACCTGAAGAAGGGCTACATGATAGTCAAGTGCAGGGGTCAGCAGGACATCCAGGAGCAG
  CTGAGCCTGGCTGAGGCTCTTCAGAAGGAGCAAGTCTTCTTCAAGGAACACCCTCAATTCAGAGTTCTTC
  TCGAGGATGGGAAGGCCACAGTGCCCTGCTTGGCAAAGAGACTGACCATGGAGCTCACCTCCCACATCTG
  TAAATCGCTGCCAATATTGGAAAATCAAATAAATGTGAATCATCAGATTGCGAGTGAGGAGCTGCAGAAG
  TACGGAGCAGACATACCAGAAGATGACAGTAAGAGGCTGTCCTTTCTGATGAATAAAATCAATGTCTTCA
  ATAAGGATATCCTAAGTTTGGTACAAGCACAAGAAAACATATCATGGGAAGAAAGCCGGCTGTTCACCAA
  ACTGCGAAATGAGTTTCTTGCTTGGAATGATTATATTGAGGAACATTTCAAAAAAACCCTAGGTTCTTCT
  GAGAAACACAGCCAGATGGAAAAATTTGAAAGTCATTATCGTGGCCGGGAACTGCCAGGGTTTGTGGACT
  ATAAGGCATTTGAGAACATTATCAAGAAGGAAGTCAAGGCCCTGGAAGAACCCGCTCTGAACATGCTGCA
  CAGGGTCACTACCATGGTCAAAAATGCCTTCACAAAGGTTTCATCAAACAATTTTGGTGATTTTTTAAAC
  CTCCACAGTACTGCCAAGTCCAAAATTGAAGACATCAGATTTAGCCAAGAAAAAGAAGCCGAAAAACTGA
  TCCGACTTCACTTCCAGATGGAACACATTGTCTACTGCCAGGACCAGGCTTACAAGAAAGCCTTGAAGGA
  GATCAGAGAGAAGGAAGCTGAGAAAGAGAAGTCAACCTTTGGTGTCTTTCAACAAAACTCTCCTCGAAAG
  GAGTTGACTACCACTGAGATGACCCAGCACCTGAATGCCTACTACCAGGAGTGCGGAAGGAACATTGGGA
  GACAGATCCCTCTGATCATCCAGTACTCCATCCTGCAAACATTTGGGCAGGAAATGGAGAAAGCAATGCT
  TCAGCTTCTACAGGACACCAGTAAGTGCAACTGGTTCCTGACTGAGCAGAGTGACAGCAGAGAGAAGAAG
  AAGTTCCTGAAGAGGCGGCTTTTAAGGCTGGATGAGGCTCAGCGGAAGCTTGCCAAATTCTCCAATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271060
• Insert Size: 1959 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271060.1, NP_001257989.1
• RefSeq Size: 3238 bp
• RefSeq ORF: 1959 bp
• Locus ID: 24575
• Cytogenetics: 11q12
• Gene Summary: mouse homolog is an interferon inducible gene that confers influenza resistance to mice [RGD, Feb 2006]
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. All six variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.