Adcyap1r1 (NM_001270583) Rat Untagged Clone

CAT#: RN216784

Adcyap1r1 (untagged) - Rat adenylate cyclase activating polypeptide 1 receptor 1 (Adcyap1r1), transcript variant 6

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Adcyap1r1
• Synonyms: PAC1-R; PACAP-R1; PACAP-R1A; PACAPR1; PACAPR1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216784 representing NM_001270583
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGAGTCCTGCAGCTCTCCCTGACTGCTCTCCTGCTGCCTGTGGCTATTGCTATGCACTCTGACT
  GCATCTTCAAGAAGGAGCAAGCCATGTGCCTGGAGAGGATCCAGAGGGCCAACGACCTGATGGGACTAAA
  CGAGTCTTCCCCAGGTTGCCCTGGCATGTGGGACAATATCACATGTTGGAAGCCAGCTCAAGTAGGTGAG
  ATGGTCCTTGTAAGCTGCCCTGAGGTCTTCCGGATCTTCAACCCGGACCAAGTCTGGATGACAGAAACCA
  TAGGAGATTCTGGTTTTGCCGATAGTAATTCCTTGGAGATCACAGACATGGGGGTCGTGGGCCGGAACTG
  CACAGAGGACGGCTGGTCGGAGCCCTTCCCCCACTACTTCGATGCTTGTGGGTTTGATGATTATGAGCCT
  GAGTCTGGAGATCAGGATTATTACTACCTGTCGGTGAAGGCTCTCTACACAGTCGGCTACAGCACTTCCC
  TCGCCACCCTCACTACTGCCATGGTCATCTTGTGCCGCTTCCGGAAGCTGCATTGCACTCGCAACTTCAT
  CCACATGAACCTGTTTGTATCCTTCATGCTGAGGGCTATCTCCGTCTTCATCAAGGACTGGATCTTGTAC
  GCCGAGCAGGACAGCAGTCACTGCTTCGTTTCCACCGTGGAGTGCAAAGCTGTCATGGTTTTCTTCCACT
  ACTGCGTGGTGTCCAACTACTTCTGGCTGTTCATTGAAGGCCTGTACCTCTTTACACTGCTGGTGGAGAC
  CTTCTTCCCTGAGAGGAGATATTTCTACTGGTACACCATCATCGGCTGGGGGACACCTACTGTGTGTGTA
  ACAGTGTGGGCTGTGCTGAGGCTCTATTTTGATGATGCAGGATGCTGGGATATGAATGACAGCACAGCTC
  TGTGGTGGGTGATCAAAGGCCCCGTGGTTGGCTCTATAATGGTTAACTTTGTGCTTTTCATCGGCATCAT
  CATCATCCTTGTACAGAAGCTGCAGTCCCCAGACATGGGAGGCAACGAGTCCAGCATCTACTTCTGCGTG
  CAGAAATGCTACTGCAAGCCACAGCGGGCTCAGCAGCACTCTTGCAAGATGTCAGAACTATCCACCATTA
  CTCTACGGCTGGCCCGCTCCACCCTACTGCTCATCCCACTCTTCGGAATCCACTACACAGTATTCGCCTT
  CTCTCCAGAGAACGTCAGCAAGAGGGAAAGACTTGTGTTTGAGCTTGGGCTGGGCTCCTTCCAGGGCTTT
  GTGGTGGCTGTACTCTACTGCTTCTTGAATGGGGAGGTACAGGCAGAGATTAAGAGGAAATGGAGGAGCT
  GGAAGGTGAACCGTTACTTCACTATGGACTTCAAGCACCGGCACCCGTCCCTGGCCAGCAGTGGAGTAAA
  TGGGGGAACCCAGCTGTCCATCCTGAGCAAGAGCAGCTCCCAGCTCCGCATGTCCAGCCTCCCGGCCGAC
  AACTTGGCCACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270583
• Insert Size: 1485 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270583.1, NP_001257512.1
• RefSeq Size: 6232 bp
• RefSeq ORF: 1485 bp
• Locus ID: 24167
• UniProt ID: P32215
• Cytogenetics: 4q24
• Gene Summary: regulates neural precursor proliferation; mediates inhibitory signaling for Shh-induced cerebellar granule precursor cell proliferation [RGD, Feb 2006]
  Transcript Variant: This variant (6) lacks an alternate in-frame exon and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant (1). The resulting isoform (e) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.