Pax3 (NM_053710) Rat Untagged Clone

CAT#: RN216768

Pax3 (untagged) - Rat paired box 3 (Pax3)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pax3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216768 representing NM_053710
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACGCTGGCCGGCGCTGTGCCCAGGATGATGCGGCCCGGCCCGGGGCAGAATTACCCACGCAGCG
  GCTTCCCACTGGAAGTGTCTACACCCCTCGGCCAGGGCCGAGTCAATCAGCTCGGAGGAGTATTTATCAA
  CGGCAGGCCTCTGCCCAACCACATCCGCCACAAGATCGTGGAGATGGCCCACCACGGCATTCGGCCTTGC
  GTCATCTCTCGCCAACTTCGCGTGTCCCACGGCTGTGTCTCTAAGATCTTATGCAGGTACCAGGAGACAG
  GCTCCATCCGACCTGGCGCCATTGGCGGCAGCAAGCCCAAGCAGGTGACAACGCCTGACGTGGAGAAGAA
  AATTGAGGAATACAAAAGAGAGAACCCAGGCATGTTTAGCTGGGAAATCAGAGACAAATTACTCAAGGAC
  GCTGTCTGTGATCGGAACACTGTGCCCTCAGTGAGTTCCATCAGTCGCATCCTGAGGAGTAAATTTGGAA
  AAGGAGAAGAGGAGGAGGCGGATTTAGAAAGGAAAGAAGCCGAGGAAAGCGAGAAAAAGGCTAAACACAG
  CATCGATGGCATCCTAAGTGAGCGAGCCTCCGCACCTCAATCAGATGAAGGCTCGGACATTGACTCAGAA
  CCTGATTTACCGCTGAAGAGGAAGCAGCGCAGGAGTAGAACCACCTTCACCGCAGAGCAGCTGGAGGAAC
  TGGAGCGCGCTTTCGAGAGAACCCACTATCCAGACATTTACACCAGGGAGGAGCTGGCCCAGAGGGCGAA
  GCTTACTGAGGCCCGAGTGCAGGTCTGGTTTAGCAACCGCCGTGCAAGATGGAGGAAGCAAGCTGGAGCC
  AATCAACTGATGGCTTTCAACCATCTCATTCCGGGGGGATTCCCTCCCACCGCCATGCCGACCCTGCCAA
  CATACCAGCTGTCGGAGACCTCTTACCAGCCCACGTCTATTCCACAAGCCGTATCAGATCCCAGTAGTAC
  TGTTCACAGACCTCAGCCGCTTCCTCCGAGCACTGTCCACCAAAGCACCATTCCTTCGAATGCAGACAGC
  AGCTCTGCCTACTGCCTTCCCAGCACCAGGCATGGATTTTCAAGCTACACCGACAGCTTCGTGCCTCCAT
  CGGGGCCCTCCAACCCCATGAACCCCGCCATTGGCAATGGCCTTTCACCTCAGGTAATGGGACTTCTGAC
  CAACCACGGTGGGGTACCGCACCAGCCTCAGACCGACTATGCTCTCTCCCCTCTCACTGGGGGCCTGGAA
  CCCACGACCACGGTGTCAGCCAGCTGCAGTCAGAGATTGGAGCACATGAAGAATGTGGACAGTCTGCCCA
  CATCTCAGCCCTACTGTCCCCCCACCTATAGTACCACAGGCTACAGCATGGACCCTGTCACGGGCTACCA
  GTATGGGCAGTATGGACAAAGTGCCTTTCATTATCTCAAGCCAGATATTGCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053710
• Insert Size: 1455 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053710.1, NP_446162.1
• RefSeq Size: 2197 bp
• RefSeq ORF: 1455 bp
• Locus ID: 114502
• Cytogenetics: 9q33
• Gene Summary: may act as a transcription factor and play a role in Schwann cell development; may be regulated by transcriptional modulator Sox10 [RGD, Feb 2006]