Hnf4a (NM_001270933) Rat Untagged Clone

CAT#: RN216675

Hnf4a (untagged) - Rat hepatocyte nuclear factor 4, alpha (Hnf4a), transcript variant 3

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hnf4a
• Synonyms: Hnf4; Hnf4alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216675 representing NM_001270933
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGACAGGGGCCAGGGTTTCCTGCAGGACCCACCAGACACATCCCCATCTGAAGGTGCCAACCTCA
  ACTCATCCAACAGCCTGGGTGTCAGTGCCCTGTGTGCCATCTGTGGCGATCGGGCCACTGGCAAACACTA
  CGGAGCCTCAAGCTGTGACGGCTGCAAGGGATTCTTCAGGAGGAGCGTGAGGAAGAACCACATGTACTCC
  TGCAGGTTTAGCAGGCAGTGCGTGGTAGACAAAGATAAGAGGAACCAGTGTCGTTACTGCAGGCTCAAGA
  AGTGCTTCCGGGCTGGCATGAAGAAAGAAGCCGTCCAAAATGAGCGGGATCGGATCAGCACGCGGAGGTC
  AAGCTACGAGGACAGCAGCCTACCCTCCATTAATGCGCTCCTGCAGGCAGAGGTCCTGTCTCAGCAGATC
  ACCTCCCCCATCTCTGGGATCAATGGCGACATTCGGGCCAAGAGGATTGCCAACATCACGGATGTGTGTG
  AGTCTATGAAGGAGCAGCTGCTGGTTCTGGTCGAATGGGCCAAGTACATCCCGGCCTTCTGTGAACTTCT
  TCTGGATGACCAGGTGGCGCTGCTCAGAGCCCACGCTGGTGAGCACCTGCTGCTTGGAGCCACCAAGAGG
  TCCATGGTGTTCAAGGATGTGCTGCTCCTAGGCAATGACTACATCGTCCCTCGGCACTGTCCAGAGCTAG
  CAGAGATGAGCCGTGTGTCCATTCGCATCCTCGATGAGCTGGTCTTGCCCTTCCAAGAGCTGCAGATCGA
  TGATAATGAATACGCCTGCCTCAAAGCCATCATCTTCTTTGACCCAGATGCCAAGGGGCTGAGTGACCCA
  GGCAAGATCAAGCGGCTGCGGTCACAGGTGCAGGTGAGCCTGGAGGATTACATCAACGACCGGCAGTATG
  ACTCTCGGGGTCGTTTTGGAGAGCTGCTGCTGCTCCTGCCCACTCTGCAGAGCATTACCTGGCAGATGAT
  CGAGCAGATCCAGTTCATCAAGCTCTTTGGCATGGCCAAGATTGACAACCTGCTGCAGGAGATGCTGCTT
  GGAGGGTCTGCCAGTGACGCGCCCCACGCCCACCACCCCCTGCACCCTCACCTGATGCAAGAACACATGG
  GCACCAATGTCATAGTTGCCAACACGATGCCCTCTCACCTCAGCAATGGACAGATGTCCACCCCTGAGAC
  TCCACAGCCATCACCACCAAGTGGCTCTGGATCTGAATCCTACAAGCTCCTGCCAGGAGCCATCACCACC
  ATCGTCAAGCCTCCCTCTGCCATCCCCCAGCCAACGATCACCAAGCAGGAAGCCATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270933
• Insert Size: 1320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270933.1, NP_001257862.1
• RefSeq Size: 4426 bp
• RefSeq ORF: 1320 bp
• Locus ID: 25735
• Cytogenetics: 3q42
• Gene Summary: steriod horomone receptor; involved in promoting transcription [RGD, Feb 2006]
  Transcript Variant: This variant (3) differs in the 5' UTR and has two coding region differences, compared to variant 1. The resulting isoform (3, also known as HNF4alpha10) is shorter, has a distinct N-terminus and lacks an internal segment in the C-terminal region, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.