Nrg1 (NM_031588) Rat Untagged Clone

CAT#: RN215433

Nrg1 (untagged ORF) - Rat neuregulin 1 (Nrg1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nrg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN215433 representing NM_031588
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGCGCAAAGAAGGCAGAGGCAAGGGGAAGGGCAAGAAGAAGGACCGGGGATCCCGCGGGAAGC
  CCGGGCCCGCCGAGGGCGACCCGAGCCCAGCACTGCCTCCCAGATTGAAAGAAATGAAGAGCCAGGAGTC
  AGCTGCAGGCTCCAAGCTAGTGCTCCGGTGCGAAACCAGCTCCGAGTACTCCTCACTCAGATTCAAATGG
  TTCAAGAATGGGAACGAGCTGAACCGCAAAAATAAACCAGAAAACATCAAGATACAGAAGAAGCCAGGGA
  AGTCAGAGCTTCGAATTAACAAAGCATCCCTGGCTGACTCTGGAGAGTATATGTGCAAAGTGATCAGCAA
  GTTAGGAAATGACAGTGCCTCTGCCAACATCACCATTGTTGAGTCAAACGAGTTCATCACTGGCATGCCA
  GCCTCGACTGAGACAGCCTATGTGTCCTCAGAGTCTCCCATTAGAATCTCAGTTTCAACAGAAGGCGCAA
  ACACTTCTTCATCCACATCGACATCCACGACTGGGACCAGCCATCTCATAAAGTGCGCGGAGAAGGAGAA
  AACTTTCTGTGTGAATGGGGGCGAGTGCTTCACGGTGAAGGACCTGTCAAACCCGTCAAGATACTTGTGC
  AAGTGCCCAAATGAGTTTACTGGTGATCGTTGCCAAAACTACGTAATGGCCAGCTTCTACAAAGCGGAGG
  AACTCTACCAGAAGAGGGTGCTGACAATTACTGGCATCTGTATCGCCCTGCTGGTGGTCGGCATCATGTG
  TGTGGTGGCCTACTGCAAAACCAAGAAGCAGCGGCAGAAGCTTCATGATCGGCTTCGGCAGAGTCTTCGG
  TCAGAACGGAGCAACCTGGTGAACATAGCGAATGGGCCTCACCACCCAAACCCACCGCCAGAGAACGTGC
  AGCTGGTGAATCAATACGTATCTAAAAACGTCATCTCCAGTGAGCATATTGTTGAGAGAGAAGTGGAGAC
  TTCCTTTTCCACCAGTCATTACACTTCCACAGCCCATCACTCCACGACTGTCACCCAGACTCCTAGTCAC
  AGCTGGAGTAATGGGCACACGGAGAGCATCATTTCAGAAAGCAACTCCGTAATCATGATGTCTTCGGTAG
  AGAACAGCAGGCACAGCAGTCCCGCCGGGGGCCCACGAGGACGTCTTCATGGCCTGGGAGGCCCTCGTGA
  TAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGACTCCTACAGAGACTCTCCTCATAGCGAAAGGTAT
  GTATCAGCCATGACCACCCCGGCTCGTATGTCACCTGTAGATTTCCACACGCCAAGCTCCCCTAAATCGC
  CCCCTTCGGAAATGTCTCCACCCGTGTCCAGCATGACGGTGTCAATGCCCTCTGTGGCAGTCAGCCCCTT
  TGTGGAAGAAGAGAGGCCTCTGCTGCTTGTGACGCCACCAAGGCTACGGGAGAAGAAATATGATCATCAC
  CCCCAGCAACTCAACTCCTTTCATCACAACCCTGCACATCAGAGTACCAGCCTCCCCCCTAGCCCACTGA
  GGATAGTGGAGGATGAGGAGTACGAGACGACCCAGGAGTATGAGTCAGTTCAAGAGCCCGTTAAGAAAGT
  CACCAATAGCCGGCGGGCCAAAAGAACCAAGCCCAATGGCCACATTGCCAATAGGTTGGAAATGGACAGC
  AACACAAGTTCTGTGAGCAGTAACTCAGAAAGTGAGACAGAAGACGAAAGAGTAGGTGAAGACACACCAT
  TCCTGGGCATACAGAACCCCCTGGCAGCCAGCCTTGAGGTGGCCCCTGCCTTCCGTCTGGCTGAGAGCAG
  GACTAACCCAGCAGGCCGCTTCTCCACACAGGAGGAATTACAGGCCAGGCTGTCTAGTGTAATCGCTAAC
  CAAGACCCTATTGCTGTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031588
• Insert Size: 1911 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031588.2, NP_113776.2
• RefSeq Size: 3246 bp
• RefSeq ORF: 1911 bp
• Locus ID: 112400
• Cytogenetics: 16q12.3
• Gene Summary: ligand for Erbb3 and Erbb4 receptors; gene produces many different alternative splicing isoforms; involved in neural and organ development [RGD, Feb 2006]
  Transcript Variant: This variant (13) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (13) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.