Chrnb2 (NM_019297) Rat Untagged Clone

CAT#: RN213099

Chrnb2 (untagged ORF) - Rat cholinergic receptor, nicotinic, beta 2 (neuronal) (Chrnb2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Chrnb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213099 representing NM_019297
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGCACTCCAACTCAATGGCGCTGTTCAGCTTCAGCCTTCTTTGGCTGTGCTCAGGGGTTTTGG
  GAACTGACACAGAGGAGCGGCTAGTGGAGCATCTCTTAGATCCCTCCCGCTATAACAAGCTGATTCGTCC
  AGCTACTAACGGCTCTGAGCTGGTGACTGTACAGCTCATGGTATCATTGGCTCAGCTCATTAGTGTGCAC
  GAGCGGGAGCAGATCATGACCACCAATGTCTGGCTGACCCAGGAGTGGGAAGATTACCGCCTCACATGGA
  AGCCTGAGGACTTCGACAATATGAAGAAAGTCCGGCTCCCTTCCAAACACATCTGGCTCCCAGATGTGGT
  TCTATACAACAATGCTGACGGCATGTACGAAGTCTCCTTCTATTCCAATGCTGTGGTCTCCTATGATGGC
  AGCATCTTTTGGCTACCACCTGCCATCTACAAGAGTGCATGCAAGATTGAGGTGAAGCACTTCCCATTTG
  ACCAGCAGAATTGCACCATGAAGTTTCGCTCATGGACCTACGACCGTACTGAGATTGACCTGGTGCTCAA
  AAGTGATGTGGCCAGTCTGGATGACTTCACACCCAGCGGGGAGTGGGACATCATCGCACTGCCAGGCCGA
  CGCAACGAGAACCCAGACGACTCCACCTATGTGGACATCACCTATGACTTCATCATTCGTCGCAAACCAC
  TCTTCTACACTATCAACCTCATCATCCCCTGCGTACTCATCACCTCGCTGGCCATCCTGGTCTTCTACCT
  GCCCTCAGACTGTGGTGAAAAGATGACACTTTGTATTTCTGTGCTGCTAGCACTCACGGTGTTCCTGCTG
  CTCATCTCCAAGATTGTGCCTCCCACCTCCCTCGATGTACCGCTGGTGGGCAAGTACCTCATGTTTACCA
  TGGTGCTAGTCACCTTCTCCATCGTCACCAGCGTGTGTGTGCTCAATGTGCACCACCGCTCGCCTACCAC
  GCACACCATGGCCCCCTGGGTCAAGGTGGTCTTCCTGGAGAAGCTGCCCACCCTGCTCTTCCTGCAGCAG
  CCACGCCACCGCTGTGCACGTCAGCGTCTGCGCTTGAGGAGGCGCCAGCGAGAGCGTGAGGGCGCAGGCG
  CGCTTTTCTTCCGTGAAGGTCCTGCGGCTGACCCATGTACCTGCTTTGTCAACCCTGCATCAGTGCAGGG
  CTTGGCTGGGGCTTTCCGAGCTGAGCCCACTGCAGCCGGCCCGGGGCGCTCTGTGGGGCCATGCAGCTGT
  GGCCTCCGGGAAGCAGTGGATGGCGTACGCTTCATTGCGGACCACATGCGAAGTGAGGATGATGACCAGA
  GTGTGAGGGAGGACTGGAAATACGTTGCCATGGTGATCGACCGCCTGTTCCTGTGGATCTTTGTCTTTGT
  CTGTGTCTTTGGGACCGTCGGCATGTTCCTGCAGCCTCTCTTCCAGAACTACACTGCCACTACCTTCCTC
  CACCCTGACCACTCAGCTCCCAGCTCCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019297
• Insert Size: 1503 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019297.1, NP_062170.1
• RefSeq Size: 2197 bp
• RefSeq ORF: 1503 bp
• Locus ID: 54239
• UniProt ID: P12390
• Cytogenetics: 2q34
• Gene Summary: co-assembles with the alpha 7 subunit to form a functional heteromeric nicotinic acetylcholine receptor [RGD, Feb 2006]