Hpse (NM_022605) Rat Untagged Clone

CAT#: RN212911

Hpse (untagged ORF) - Rat heparanase (Hpse), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hpse
• Synonyms: Hep; Hpa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212911 representing NM_022605
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGCCGCTGCTGCTGCTGTGGCTCTGGGGGCGGCTCCGTGCCCTGACCCAAGGCACTCCGGCGG
  GGACCGCGCCGACCAAGGACGTGGTGGACTTGGAGTTTTACACCAAGAGGCTATTCCAAAGCGTGAGTCC
  CTCGTTCCTGTCCATCACCATCGACGCCAGCCTGGCCACCGACCCTCGGTTCCTCACCTTCCTGGGCTCT
  CCACGGCTTCGAGCCCTGGCTAGAGGCTTATCTCCTGCGTACTTGAGATTTGGCGGCACCAAGACTGACT
  TCCTTATTTTTGATCCCAACAAGGAACCCACCTCTGAAGAAAGAAGTTACTGGCAATCTCAAGACAACAA
  TGATATTTGCGGGTCTGAGCGGGTCTCTGCTGACGTGTTGAGGAAACTCCAGATGGAGTGGCCCTTCCAG
  GAGCTGCTGCTGCTCCGAGAACAGTACCAAAGAGAATTCAAGAACAGCACCTACTCACGAAGCTCGGTGG
  ACATGCTCTACAGTTTTGCTAAGTGCTCGAGGTTAGACCTGATCTTTGGTCTAAATGCGTTACTAAGAAC
  CCCAGACTTGCGGTGGAACAGCTCCAACGCCCAGCTTCTCCTCAACTACTGCTCTTCCAAGGGTTATAAC
  ATCTCCTGGGAACTGGGCAACGAGCCCAACAGTTTCTGGAAGAAAGCTCAGATTTCCATCGATGGGTTGC
  AGCTAGGAGAAGACTTTGTGGAGTTGCATAAACTTCTACAAAAGTCAGCTTTCCAAAACGCAAAACTCTA
  TGGTCCTGACATTGGTCAGCCTCGAGGGAAGACAGTTAAGCTGCTGAGAAGCTTCCTGAAGGCTGGTGGA
  GAAGTGATTGACTCTCTCACCTGGCATCACTACTACTTGAATGGACGAGTTGCGACCAAAGAAGATTTTC
  TGAGCTCTGATGTCCTGGACACTTTTATCCTATCTGTGCAAAAAATTCTGAAGGTGACTAAGGAGATGAC
  ACCTGGCAAGAAGGTCTGGTTGGGAGAGACGAGCTCTGCCTACGGCGGCGGAGCGCCCTTGCTGTCCAAT
  ACCTTTGCAGCTGGCTTTATGTGGCTGGATAAATTGGGCCTGTCAGCCCAGCTGGGGATAGAAGTCGTGA
  TGAGGCAGGTGTTTTTCGGAGCAGGCAACTACCACTTAGTGGACGAAAACTTCGAGCCCTTGCCCGATTA
  CTGGCTCTCTCTCCTGTTCAAGAAACTGGTAGGTCCCAAGGTGTTAATGTCAAGAGTGAAAGGCCCAGAC
  AGAAGCAAACTCCGAGTGTACCTCCACTGCACGAACGTCTATCACCCAAGGTATCGGGAAGGAGATTTAA
  CTCTGTACGTCCTGAACCTCCATAATGTCACCAAGCACTTGAAGCTGCCGCCTCCGATGTTCAGCAGACC
  GGTGGATAAGTACCTGCTGAAGCCTTTCGGTTCTGACGGACTGCTTTCCAAATCCGTCCAACTGAACGGT
  CAAACCCTGAAGATGGTCGATGAGCAGACCCTGCCAGCTCTAACAGAAAAACCTCTCCCCGCAGGAAGCT
  CACTAAGCGTGCCCGCCTTTTCCTATGGGTTTTTTGTCATAAGAAATGCCAAAATCGCAGCTTGTATATG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022605
• Insert Size: 1611 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022605.1, NP_072127.1
• RefSeq Size: 3177 bp
• RefSeq ORF: 1611 bp
• Locus ID: 64537
• UniProt ID: Q71RP1
• Cytogenetics: 14p22
• Gene Summary: endo-beta-glucuronidase capable of cleaving heparan sulfate involved in the regulation of heparin sulfate proteoglycan function [RGD, Feb 2006]