Prkn (NM_020093) Rat Untagged Clone

CAT#: RN212553

Park2 (untagged ORF) - Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Prkn
• Synonyms: Park; Park2; Prkn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212553 representing NM_020093
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGTGTTTGTCAGGTTCAACTCCAGCTATGGCTTCCCAGTGGAGGTCGATTCTGACACCAGCATCT
  TCCAGCTCAAGGAAGTGGTTGCTAAGCGACAGGGGGTTCCAGCTGACCAGCTGCGAGTGATTTTTGCTGG
  GAAGGAGCTGCAGAATCACCTGACAGTACAGAACTGTGACCTGGAACAACAGAGTATCGTTCACATAGTA
  CAGAGACCACAGAGGAAAAGTCACGAAACAAATGCCTCTGGAGGGGACAAACCCCAGAGCACCCCGGAGG
  GCTCCATATGGGAGCCCAGAAGCTTGACTCGAGTGGACCTCAGCAGCCATATCCTGCCAGCGGACTCCGT
  GGGGCTGGCAGTCATTCTGGACACAGACAGCAAGAGTGACTCAGAAGCAGCCAGAGGTCCAGCAGCTAAA
  CCCACCTACCACAGCTTTTTTGTCTACTGCAAAGGCCCCTGCCACAAGGTCCAGCCTGGGAAACTCCGAG
  TTCAGTGCGGCACCTGCAGACAAGCAACCCTCACCTTGGCCCAGGGCCCATCTTGCTGGGATGATGTCTT
  AATTCCAAACCGGATGAGTGGAGAGTGCCAATCTCCAGACTGCCCTGGGACAAGAGCTGAATTTTTCTTT
  AAATGTGGAGCACACCCAACCTCAGACAAGGACACATCAGTAGCTTTGAACCTGATCACCAACAACAGCC
  GCAGCATCCCCTGCATCGCGTGCACGGATGTCAGGAACCCTGTCTTGGTCTTCCAATGTAACCACCGCCA
  CGTGATCTGTTTGGACTGCTTCCACTTGTACTGTGTCACAAGGCTCAACGATCGGCAGTTTGTCCACGAC
  GCTCAACTTGGCTACTCCCTGCCGTGTGTGGCTGGCTGTCCCAACTCCCTGATTAAAGAGCTCCATCACT
  TCAGGATCCTTGGAGAAGAGCAGTACAACAGGTACCAGCAGTATGGTGCCGAGGAGTGCGTGCTGCAGAT
  GGGAGGTGTGCTGTGCCCCCGTCCTGGCTGCGGAGCTGGGCTGCTGCCTGAACAGGGCCAGAGGAAAGTC
  ACCTGCGAAGGGGGCAACGGCCTGGGCTGTGGGTTCGTTTTCTGCCGGGACTGCAAGGAAGCATACCATG
  AAGGGGAGTGCGACTCGATGTTCGAAGCCTCGGGGGCGACTTCTCAGGCATACCGGGTGGATCAAAGAGC
  TGCTGAGCAAGCACGGTGGGAGGAGGCCTCCAAGGAAACCATCAAGAAAACCACCAAGCCTTGTCCTCGC
  TGCAATGTGCCCATTGAAAAGAATGGAGGATGTATGCACATGAAGTGTCCTCAGCCCCAGTGCAAGCTGG
  AGTGGTGCTGGAACTGTGGCTGTGAGTGGAACCGAGCCTGCATGGGTGATCACTGGTTTGACGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_020093
• Insert Size: 1398 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020093.1, NP_064478.1
• RefSeq Size: 1564 bp
• RefSeq ORF: 1398 bp
• Locus ID: 56816
• UniProt ID: Q9JK66
• Cytogenetics: 1q11
• Gene Summary: localized to the cytoplasm in neurons of many regions of the brain; has N-terminal ubiquitin and ring-IBR-ring finger domains [RGD, Feb 2006]