Cdk20 (NM_001025752) Rat Untagged Clone

CAT#: RN210976

Cdk20 (untagged ORF) - Rat cell cycle related kinase (Ccrk), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cdk20
• Synonyms: Ccrk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210976 representing NM_001025752
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCAGTATTGCATCCTCGGTCGCATCGGGGAGGGCGCCCACGGCATCGTCTTTAAAGCCAAGCACG
  TAGAGACTGGCGAGATCGTTGCCCTCAAGAAGGTGGCCCTGCGGCGGCTGGAGGATGGTATTCCTAACCA
  GGCCCTCAGGGAAATCAAGGCTCTGCAGGAGATCGAGGACAGTCAATATGTGGTACAGCTGAAGGCTGTG
  TTCCCACATGGTGCAGGCTTTGTGCTGGCTTTCGAGTTTATGCTGTCGGACCTGGCGGAGGTGGTGCGCC
  ATGCCCAGAGGCCACTAGCCCCAGCACAGGTTAAGAGCTACCTGCAGATGCTGCTCAAAGGTGTTGCATT
  TTGCCATGCCAACAACATCGTGCATCGGGACCTGAAGCCTGCCAACCTGCTCATCAGTGCCTCAGGCCAG
  CTCAAGATAGCTGACTTTGGACTGGCCCGGGTCTTCTCTCCAGATGGTGGTCGCCTCTACACACATCAGG
  TGGCCACCAGGTGGTACCGAGCTCCTGAGCTCCTGTACGGTGCTCGGCAGTATGACCAGGGTGTTGACCT
  ATGGGCTGTAGGCTGTATTATGGGAGAGCTGTTGAATGGATCCCCCCTATTCCCGGGAGAAAACGACATT
  GAACAACTGTGCTGTGTGCTTCGAATCCTGGGTACCCCGAGTCCTCGAGTCTGGCCGGAGATCACAGAGC
  TGCCTGACTACAACAAGATCTCCTTCAAGGAGCAGGCACCAGTGCCCCTGGAGGAGGTGCTGCCTGATGC
  CTCTCACCAGGCCCTGGACCTGCTGGGTCAGTTCCTCCTTTACCCTCCACGACAGCGTATTGCAGCCTCC
  CAGGCCCTTCTGCATCAGTATTTCTTCACAGCTCCTCTGCCTGCCCATCCGTCTGAGCTGCCAATTCCTC
  AGCGCCCAGGGGGACCCACACCCAAGGCTCATCCAGGGCCCCCCCATGTCCATGACTTCCATGTGGATCG
  TCCTCTTGAGGAGTCACTGTTGAATCCAGAACTGATCCGGCCCTTCATCCCAGAGGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025752
• Insert Size: 1041 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025752.2, NP_001020923.2
• RefSeq Size: 2145 bp
• RefSeq ORF: 1041 bp
• Locus ID: 364666
• UniProt ID: Q4KM34
• Cytogenetics: 17p14
• Gene Summary: Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling. Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160' (By similarity).[UniProtKB/Swiss-Prot Function]