Irx1 (NM_001107331) Rat Untagged Clone

CAT#: RN210859

Irx1 (untagged ORF) - Rat iroquois homeobox 1 (Irx1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Irx1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210859 representing NM_001107331
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTCCCGCAGCTGGGCTACCCGCAATACCTAAGCGCCGCGGGCCCGGGCGCCTACGGTGGAGAGC
  GCCCTGGGATGCTGGCTGCGGCCGCGGCTGCTGCAGCCGCCGCCTCGTCGGGTCGGCCGGGGACTGCAGA
  GCTGGGAGCGGGCGCCGGAGCGGCCGCGGTCACCTCGGTGCTGGGCATGTATGCCGCGGCGGGACCGTAC
  GCGGGCGCACCCAACTACAGCGCCTTTCTGCCCTATGCCGCGGACCTCAGCCTCTTCTCACAGATGGGCT
  CTCAGTATGAACTCAAGGACAACCCCGGGGTACACCCAGCTACCTTCGCAGCCCACACAACGCCTGCTTA
  TTATCCCTATGGCCAGTTCCAATACGGGGATCCCGGACGGCCCAAGAACGCCACGCGCGAGAGCACAAGC
  ACACTGAAAGCCTGGCTCAACGAACACCGCAAGAATCCGTACCCCACCAAAGGAGAGAAGATCATGCTGG
  CCATTATTACCAAGATGACCCTCACACAGGTCTCCACCTGGTTCGCCAACGCGCGCCGGCGCCTCAAGAA
  GGAAAACAAGGTGACTTGGGGGGCGCGCAGCAAGGACCAGGAAGACGGCGCCCTCTTTGGCAGCGACACC
  GAGGGCGACCCCGAGAAGGCCGAGGACGACGAGGAGATCGACCTGGAGAGCATTGACATCGATCAGATTG
  ACGAACGTGATGGCGACCAGAGCAACGAAGACGAGGAGGACAAGGTGGAGGCTCCTAGGGCGCGCGTAGC
  CCCTCCCGCCCCAGCTCGGGACCAGAGCTCCCCGTTGTCGGCCGCCGAGACACTCAAGTCCCAAGATTCG
  CCCTTGGGCCTGGCTAAGGAGGTCTCAGAGCCGGGCAGCACGCGCCTTCTGAGTCCCGGAGCCGCGGCGG
  TCGGCCTGCAGGGCGCGCCTCACAGCAAGCCCAAGATCTGGTCACTGGCTGAGACAGCCACTAGCCCCGA
  CGGTGCGCCTAAGGCGTCCCCACCGCCACCCTCCAGCCACGCCAGCGCACACGGGCCGCCCAGCGGCTCG
  CCCCTGCAACACCCGGCCTTTCTACCCAGCCACGGACTGTACACCTGCCACATAGGCAAGTTTTCCAACT
  GGACCAACGGCGCGTTCCTAGCACAGGGCTCGCTGTTGAACATGCGATCTTTCCTGGGAGTCAGTGCACC
  GCATGCAGCACCTCATGGCCCACACGTGACCGCACCGCCACCTCCGCAGCTGCCTGTCCAGGTTGCCACA
  GGGGTGCTCCATGGTGAGAAGGCCTCGGCGAGTAGCAGTCCTGCGCTTCCAGAGAGAGACCTAGTCACCA
  GGCCGGATTCGCCCCCACAGCAGCTAAAGTCGCCCTTCCAGCCGGTGCGCGACAACTCGCTGGCCCCGCA
  GGAGGGAACGCCTCGGATCCTCGCAGCCCTCTCGTCTGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001107331
• Insert Size: 1443 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107331.1, NP_001100801.1
• RefSeq Size: 2333 bp
• RefSeq ORF: 1443 bp
• Locus ID: 306659
• Cytogenetics: 1p11