Pah (NM_012619) Rat Untagged Clone

CAT#: RN210811

Pah (untagged ORF) - Rat phenylalanine hydroxylase (Pah), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pah
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210811 representing NM_012619
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTGTTGTCCTGGAGAATGGAGTCCTGAGCAGAAAACTCTCAGACTTTGGGCAGGAAACAAGCT
  ATATTGAAGACAACTCCAATCAAAATGGTGCCATATCTCTGATATTCTCACTCAAAGAGGAAGTTGGTGC
  CCTAGCCAAGGTCCTGCGCTTATTTGAGGAGAACGATATCAACCTGACACACATTGAATCCAGACCTTCT
  CGTTTAAACAAAGATGAGTATGAGTTCTTCACTTATCTGGATAAGCGTAGCAAACCTGTTCTGGGCAGCA
  TCATCAAGAGCCTGAGGAATGACATTGGTGCCACTGTCCACGAGCTTTCCCGAGACAAGGAAAAGAACAC
  AGTGCCCTGGTTCCCAAGGACCATTCAGGAGTTGGACCGATTCGCCAATCAGATTCTCAGCTATGGAGCC
  GAACTGGATGCGGACCACCCAGGCTTTAAAGATCCTGTGTACCGGGCGAGACGAAAGCAGTTTGCTGACA
  TTGCCTACAACTACCGCCATGGCCAGCCCATCCCTCGGGTGGAATACACAGAGGAAGAGAAGCAGACCTG
  GGGAACGGTGTTCAGGACCTTGAAGGCCTTGTATAAAACACATGCCTGCTACGAGCACAACCACATTTTC
  CCACTTCTGGAAAAGTACTGCGGTTTCCGTGAAGACAACATTCCACAGCTGGAAGATGTTTCTCAATTTC
  TGCAGACTTGTACTGGTTTCCGCCTCCGACCTGTTGCTGGCTTACTGTCATCTCGAGATTTCTTGGGTGG
  CCTGGCCTTCCGAGTCTTCCACTGCACACAGTACATTAGGCATGGATCGAAGCCCATGTACACACCTGAA
  CCTGACATCTGCCATGAACTCTTGGGACATGTGCCTTTGTTTTCAGATCGCAGCTTTGCCCAGTTTTCTC
  AGGAAATTGGGCTTGCATCACTGGGGGCACCTGATGAGTACATTGAGAAACTGGCCACAATTTACTGGTT
  TACTGTGGAGTTTGGGCTTTGCAAGGAAGGAGATTCTATAAAGGCATATGGTGCTGGGCTCTTGTCATCC
  TTTGGAGAATTACAGTACTGTTTATCAGACAAGCCAAAGCTCCTCCCCCTGGAGCTAGAGAAGATAGCCT
  GCCAGGAGTATTCTGTCACAGAGTTCCAGCCCTTGTACTACGTGGCGGAGAGTTTCAGTGATGCCAAGGA
  GAAAGTGAGGACTTTTGCTGCCACGATCCCTCGGCCCTTCTCGGTTCGCTATGACCCTTACACTCAAAGG
  GTTGAGGTCCTGGACAATACTCAGCAGTTGAAGATTTTAGCTGACTCCATTAATAGTGAGGTCGGAATCC
  TTTGCAATGCCCTGCAGAAGATAAAGTCGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012619
• Insert Size: 1362 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012619.2, NP_036751.2
• RefSeq Size: 2040 bp
• RefSeq ORF: 1362 bp
• Locus ID: 24616
• Cytogenetics: 7q13
• Gene Summary: catalyzes the conversion of phenylalanine to tyrosine; deficiency in humans causes genetic disorder phenylketonuria [RGD, Feb 2006]