Pten (NM_031606) Rat Untagged Clone

CAT#: RN210687

Pten (untagged ORF) - Rat phosphatase and tensin homolog (Pten), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pten
• Synonyms: Mmac; MMAC1; TEP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210687 representing NM_031606
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAG
  ACTTGACCTATATTTATCCAAATATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAGGTGTATACAG
  GAACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACCATTACAAGATATACAATCTA
  TGTGCTGAGAGACATTATGACACCGCCAAATTTAACTGCAGAGTTGCACAGTATCCTTTTGAAGACCATA
  ACCCACCACAGCTAGAACTTATCAAACCCTTTTGTGAAGATCTTGACCAATGGCTAAGTGAAGACGACAA
  TCATGTTGCAGCAATTCACTGTAAAGCTGGGAAAGGACGGACTGGTGTAATGATTTGTGCATATTTATTG
  CATCGGGGCAAGTTTTTAAAGGCACAAGAGGCCCTGGATTTTTATGGGGAAGTAAGGACCAGAGATAAAA
  AGGGAGTAACTATTCCCAGTCAGAGGCGCTATGTATATTATTATAGCTACCTGTTAAAGAATCACCTGGA
  TTACAGACCAGTGGCACTGTTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAACT
  TGCAATCCCCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATCTACTCCTCCAACTCAGGACCCACGCGGC
  GGGAGGACAAGCTCATGTACTTTGAGTTCCCTCAGCCATTGCCTGTGTGTGGTGACATCAAAGTAGAGTT
  CTTCCACAAACAGAACAAGATGCTCAAAAAGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATA
  CCAGGACCAGAGGAAACCTCAGAAAAAGTGGAAAATGGAAGTCTTTGTGATCAGGAAATCGATAGCATTT
  GTAGTATAGAGCGTGCGGATAATGACAAGGAGTATCTTGTGCTCACCCTGACAAAAAATGATCTTGACAA
  AGCAAACAAAGACAAGGCCAACCGATACTTCTCTCCAAATTTTAAGGTGAAGTTATACTTCACAAAAACA
  GTAGAGGAGCCATCAAATCCAGAGGCTAGCAGTTCAACTTCTGTGACTCCAGACGTTAGTGACAATGAAC
  CTGATCATTATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAACCTTTTGATGAAGATCAGCA
  TTCACAAATTACAAAAGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031606
• Insert Size: 1212 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031606.1, NP_113794.1
• RefSeq Size: 1212 bp
• RefSeq ORF: 1212 bp
• Locus ID: 50557
• Cytogenetics: 1q52
• Gene Summary: may be involved in regulation of phosphoinositide 3-kinase and Akt kinase mediated antiapoptotic pathways [RGD, Feb 2006]