Bbox1 (NM_022629) Rat Untagged Clone

CAT#: RN210428

Bbox1 (untagged ORF) - Rat butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (Bbox1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Bbox1
• Synonyms: Bbh; Bbox
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210428 representing NM_022629
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTGTGCCATCCTAAAGGCGGAAGCAGTGGATGGGGCTCGCTTGATGCAGATCTTTTGGCACGATG
  GGGCAGAGTCCCTCTACCCAGCAGTATGGCTCAGAGACAACTGTCAGTGTTCGGACTGCTATCTACATTC
  TGCAAAAGCTCGGAAACTTCTCCTGGAAGCTCTCGATGTGAACATTAGAATGGATGACTTGACATTTGAC
  CAGAAAAAGGTTTATATCACTTGGCCCAATGGCCATTACAGCGAATTTGAAGCTAATTGGCTGAAGAAAA
  GATGCTTTTCTCAGGAGGCCAGAGCAGGACTCCAAGGAGAATTATTTTTACCAGAATGTCAGTACTGGGG
  CTCAGAGCTCCAGCTGCCAACTCTGAACTTTGAAGATGTGTTAAATGATGATGACCATGCATACAAATGG
  CTTTCTAGTCTCAAGAAAGTAGGCATTGTGAGACTCACTGGAGCAGCTGACAAACGTGGCGAGATCATAA
  AACTTGGGAAAAGAATTGGCTTTCTGTACCTCACATTTTATGGACACACTTGGCAAGTGCAGGACAAGAT
  TGATGCCAACAATGTGGCTTATACAACTGGGAAGCTCAGCTTCCACACAGATTACCCAGCCCTCCACCAT
  CCACCTGGGGTTCAGCTTCTGCACTGTATCAAGCAAACAGTCACAGGAGGTGACTCTGAAATCGTAGATG
  GGTTTAATGTGTGCCAAAAACTCAAGGAGAAGAACCCCCAGGCTTTTTCTATTCTCTCCTCCACCTTTGT
  GGATTTTACAGACATTGGAGTGGATTACTGTGATTTCTCTGTGCAGTCTAAACACAAGATTATAGAGCTG
  GATGATAAAGGCCAAGTGGTTCGCATCAACTTCAACAACGCAACCAGAGATACAGTCTTCGATGTCCCCA
  TTGAAAGAGTTCAGCCTTTCTACGCTGCTCTGAAGGAGTTTGTTGACCTGATGAACAGCAAAGAATACAA
  GTACACCTTCAAGATGAATCCAGGTGATGTGATTACCTTTGACAACTGGCGCTTGCTTCATGGACGTCGC
  AGCTATGAAGCAGGAACTGAGATATCCCGCCATCTGGAAGGCGCTTATGCTGACTGGGATGTAGTGATGT
  CAAGGCTTCGTATTCTGAGACAGAGAGTCATGAATGGGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022629
• Insert Size: 1164 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022629.1, NP_072151.1
• RefSeq Size: 1164 bp
• RefSeq ORF: 1164 bp
• Locus ID: 64564
• UniProt ID: Q9QZU7
• Cytogenetics: 3q34
• Gene Summary: Catalyzes the formation of L-carnitine from gamma-butyrobetaine.[UniProtKB/Swiss-Prot Function]