Gpc5 (NM_001107285) Rat Untagged Clone

CAT#: RN210243

Gpc5 (untagged ORF) - Rat glypican 5 (Gpc5), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gpc5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210243 representing NM_001107285
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCTCGCACCTGGCGTCTGGGATGGCGCTGTCTCCTTCTCCTGGCTCTTCTTGGATCTACCCGAA
  GCGAGGGCGTGGAAAGCTGCGAAGAAGTTCGGAAACTTTTCCAGTGGAGGCTTGGGGGAGCTGTCAAGGG
  GCTGCCGGAGGCGCCGCGGGCAGGACCTGATCTTCAGGTGTGCCTATCCAAAAACCCAACGTGCTGTACC
  AGGAAGATGGAGGAGCAGTACCAGATTGCCGCTCAGCAGGATTTGCAGCAGGTGCTACAGACATCCAGCT
  CAACACTAAAGTTGCTAATATCTCGGAATGCAGCTGCTTTTCAAGAGACCCTGGAAACCCTCATCCGACA
  AGCAGAGAATTACACCAGTATCCTTTTCTGCAACACCTACCGGAACATGGCCTTGGAGGCTGCTGCTTCC
  ACTCAGGAGTTTTTCACCGATGTGGGACTCTATTTATTTGGTGCGGATGTTAACCCCGAGGAATTTGTAA
  ACAGATTTTTTGACAGTCTTTTCCCTCTGGTCTATAACCATCTCATAAACCCTGGTGTGACTGACAGTTC
  TCTGCAATACTCAGAATGCATCCGAACGGCCCGCCAGGATGTTAGTCCATTTGGCAACATCCCCAAAAGA
  GTCATGGGACAGATGGGGAGGTCACTGCTGCCCGGTCGCACATTCCTGCAGGCGCTTAATCTGGGCATTG
  AAGTCATCAACACCACGGACCATATCCACTTCTCTAAGGAGTGCAGTAGGGCCCTCCTGAAGATGCAGTA
  CTGTCCTCACTGCCAGAGCCTGACGCTCAGTAAGCCCTGTATGGGGTACTGCCTCAATGTCATCAGGGGC
  TGTCTAGCCCACATGACAGACCTTAATCCACACTGGCATGCTTATATCCGCTCCTTGGAAGAGCTGTCGG
  ATGCGATGCACGGAACCTACGACGTTGAGCACGTGCTCCTGAACTTCCACTTACTTGTTAATGATGCCAT
  GCTGCAGGCTCACCTCAATGCGCAGAAGTTACTGGATCAGGTGAATAAGATTTGCGGCCTTCCAGTGAGA
  ACAGCGACGCAGAGCCCCCGCTGTACTTTTGATCCAAGCAAAGAGAGGCATGGGATGAAGATCTCTCCAA
  GGAATGGTGAAGAGACACTTGCCAACAGAAGAAAAGAATTCATCAATAGCCTTAGGCTGCACAGGTCCTT
  CTATGGTGGCCTGGCTGACCAGCTTTGTGTTAATGAATGGGCTGCAGCTGAAGGGAGGCCCTGCTGGAAT
  GGGGAGGACATAGTGAAAAGCTATGCTCAGCGTGTGGTTGGAAATGGCGTCAAAGCCCAGTCTGCAAATC
  CTGAAGTCAGAGTCAGAGGAACTGATCCTGTGGTAAATCAGATAATTGACAAACTGAAGCATGTGATCCA
  GCTGTTACAGGGCAGATCACCCAAACCCAACAAGTGGGAGCTTCTTCATCCGGGCAGTGGTGGCGGCATG
  CTTGAGCAATCTAGTGGAGATTGTGATGACGAAGACGGTTGTGGGGGATCAGGAAGCGGAGAAGTCAAGA
  GGACCCTAAAGATCACAAACTGGATGCCAGACAGCATGAACTTCAGTGACGTCAAACAAGTTCATGGAGC
  AGACCGTGGAAGTACTCTGGATACAACTGGTGCGGGATGCGGGTCAGGAACTGAGTCTATGACGCTCCTG
  CTGATGGGGACGCTGATGTTTCTTCCCTGGCTTTGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001107285
• Insert Size: 1719 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107285.2, NP_001100755.2
• RefSeq Size: 2491 bp
• RefSeq ORF: 1719 bp
• Locus ID: 306157
• Cytogenetics: 15q23-q24