Prkd2 (NM_001013895) Rat Untagged Clone

CAT#: RN208323

Prkd2 (untagged ORF) - Rat protein kinase D2 (Prkd2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Prkd2
• Synonyms: RGD1308054
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN208323 representing NM_001013895
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGCCCCCTCCCATCCCGCCGGACTCCCCTGCTCTCCGGGGCCCGGGTCTCCTCCGCCCCCCG
  GTGGCTCGGATCTCCAGTCTCTGCCACCTCTGCTGCCTCAGATCCCTGCTCCGGGATCGGGGGTCTCCTT
  CCACATCCAGATCGGATTAACACGAGAGTTTGTGCTGTTGCCGGCAGCTTCGGAGTTGGCTCATGTGAAG
  CAACTAGCCTGTTCTATCGTGGACCAGAAGTTCCCAGAGTGTGGCTTCTACGGCCTGTACGACAAGATCC
  TGCTCTTCAAACATGACCCAACATCAGCCAACCTCCTGCAGCTGGTGCGATCAGCTGCAGATATCCAGGA
  GGGCGACTTGGTGGAGGTGGTTCTGTCGGCCTCAGCCACCTTCGAGGATTTCCAGATCCGTCCGCATGCT
  CTCACTGTGCACTCGTACCGCGCACCAGCTTTCTGTGACCACTGTGGGGAGATGCTGTTTGGCCTTGTGC
  GCCAGGGCCTCAAGTGCGATGGCTGCGGGCTGAATTACCACAAACGCTGCGCCTTCAGCATCCCGAACAA
  CTGCAGTGGCGCTCGGAAGCGCAGACTGTCATCCACGTCTCTGGCCAGTGGCCATTCTGTGCGTCTCGGC
  AGCTCCGAGTCCCTACCCTGCACCGCCGAAGAGCTGAGCCGTAGCACCACCGACCTCCTTCCTCGCCGAC
  CACCCTCGTCGTCGTCCTCCTCCTCTTCCTCTTCCTTCTACACAGGCCGACCCATTGAGCTGGACAAGAT
  GCTGATGTCCAAGGTCAAGGTGCCACACACCTTCCTTATACATAGCTACACACGGCCCACCGTTTGCCAG
  GCTTGCAAGAAACTGCTCAAGGGTCTCTTCCGCCAGGGCCTGCAGTGCAAAGACTGCAAGTTTAACTGTC
  ACAAACGCTGTGCTACCCGAGTCCCTAATGACTGCCTGGGGGAGGCGCTCATCAATGGAGATGTGCCCAT
  GGAAGAAGCTGCTGATTACAGTGAGGCTGATAAGAGCTCCCTCTCAGATGAGTTGGAGGACTCTGGTGTC
  ATCCCAGGCTCCCATGCAGAGAATGCTCTCCATGCCAGTGAGGAAGAGGAAGGCGAGGGAGGCAAGGCCC
  AGAGCTCGCTGGGCTACATCCCCCTGATGCGTGTGGTACAGTCTGTGCGACACACGACCCGGAAATCCAG
  CACCACCCTGCGGGAGGGCTGGGTGGTCCATTACAGCAACAAGGACACACTGAGGAAGCGGCACTACTGG
  CGCCTAGACTGCAAATGCATTACCCTCTTCCAGAACAACACGACCAACAGATACTACAAGGAGATCCCGC
  TGTCTGAAATCCTCGCAGTGGAGCCGGCTCAGAACTTCAGCCTCGTACCTCCGGGCACCAACCCACACTG
  CTTTGAGATCATCACCGCCAATGTCACCTACTTTGTGGGCGAGACACCTGGCGGGGCCCCGGGAGGGCCG
  AGCGGGCAGGGAACAGAGGCTGCCCGGGGTTGGGAGACAGCCATCCGCCAGGCACTGATGCCTGTTATCC
  TCCAAGATGCACCCAGTGCCCCAGGCCACACACCACACAGACAAGCTTCTCTGAGCATCTCTGTGTCCAA
  CAGTCAGATCCAAGAGAACGTGGACATCGCCACTGTCTACCAGATCTTCCCCGATGAGGTGCTGGGCTCT
  GGACAGTTTGGAGTGGTCTATGGAGGAAAACACAGGAAGACTGGCAGAGATGTTGCAGTAAAGGTCATTG
  ACAAACTACGCTTCCCCACCAAGCAGGAGAGCCAGCTCAGGAATGAAGTGGCCATCCTCCAGAGCCTGCG
  GCACCCCGGGATTGTGAACCTCGAGTGCATGTTTGAGACCCCTGAGAAGGTATTCGTGGTGATGGAGAAA
  CTGCATGGGGACATGTTGGAGATGATCCTCTCCAGTGAGAAGGGCCGGCTTCCTGAGCGCCTCACCAAGT
  TCCTTATCACGCAGATTCTGGTGGCACTGAGACACCTACACTTCAAGAACATCGTCCACTGTGACTTGAA
  ACCAGAGAATGTGTTACTGGCATCAGCCGATCCGTTTCCTCAGGTGAAGCTATGTGACTTCGGCTTCGCT
  CGCATCATCGGTGAGAAGTCCTTCCGGCGCTCAGTGGTGGGCACGCCCGCCTACCTGGCACCTGAGGTAC
  TGCTCAACCAGGGTTACAACCGCTCGCTGGACATGTGGTCCGTGGGTGTGATCATGTATGTGAGCCTCAG
  TGGCACGTTTCCCTTCAATGAGGACGAGGACATCAATGACCAGATACAGAATGCAGCCTTTATGTACCCG
  GCCAGCCCCTGGAGCCACATCTCATCTGGAGCCATCGACCTCATCAACAACCTGTTGCAGGTGAAGATGC
  GCAAGCGCTACAGCGTGGACAAGTCTCTCAGCCACCCATGGTTACAGGAGTACCAGACGTGGCTTGACCT
  CCGAGAGCTAGAGGGAAAGATGGGCGAGCGATATATCACCCATGAGAGCGACGACGCACGCTGGGATCAG
  TTTGTATCAGAGCGCCATGGGACGCCTGCAGAAGGGGACCTGGGTGGCGCCTGTCTGCCACAGGACCATG
  AGATGCAGGGGCTGGCTGAGCGGATCAGCATCCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013895
• Insert Size: 2628 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013895.1, NP_001013917.1
• RefSeq Size: 3144 bp
• RefSeq ORF: 2628 bp
• Locus ID: 292658
• UniProt ID: Q5XIS9
• Cytogenetics: 1q21
• Gene Summary: Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 and Tyr-718 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells.[UniProtKB/Swiss-Prot Function]