Krt12 (NM_001008761) Rat Untagged Clone

CAT#: RN207743

Krt12 (untagged ORF) - Rat keratin 12 (Krt12), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Krt12
• Synonyms: Ka12; Krt1-12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207743 representing NM_001008761
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTTTCAGTCCGCACCTCTGCGCTATCCAGGAGGTCATCCTCACAGAATGGGGTGGCAGGCAGAC
  CTTGGGGTGCATCTGCTTCTAGTGTTGCCTGTGGCTATGGGGGAACAGCCTCTGGCTTTGGAGTTGGCTG
  TGGAGGGCTCCTTTCTGCGGCTTCCATGTTTGGGTCTAGTTCTGGCTTTAGCGGTGGCTCTACAGGTTGC
  TCGCCAGGGCTCGGCACTGCCTATGGGGGACCTCTGGGAGCCGGTGTGGGAGGAATGGGGATAGGGGGTA
  GCTCGGGCGGTGGCTCTCTGTGTATCTTTTCTGGCAATGATGGAGGACTTCTTTCTGGTTCTGAAAAAGA
  AACCATGCAAAATCTGAATGATAGACTAGCTTCCTACCTGGGTAAGGTTCGCGCTCTAGAGGAAGCTAAC
  GCGGAACTGGAAAACAAAATTCGAGAGTGGTATGAAACTCGAAGAACTGGAGACTCCGGGTCCCAGAGTG
  ATTACAGTAAATACTACCCGCTGATTGAGGACCTGAAGAATAAGATCATTTCTGCCAGCGTCAGCAATGC
  CCAGCTCCTCCTGCAGATTGACAACGCACGGCTGGCTGCAGAGGACTTCAGGATGAAGTATGAGAACGAG
  CTGGCCCTGCGCCAGACTGTGGAGGCCGACATCAACGGGCTGCGCAGGGTGCTGGACGAGCTGACCCTGG
  CCAGAGCCGACCTGGAAGCACAGACTGAGAACCTGACTGAGGAGCTGGCTTACATGAAGAAGAACCACGA
  GGAGGAACTCCAAAGTTTCCAGGCAGGTGGTCCAGGTGAGGTCAATGTAGAAATGGACGCCGCACCTGGA
  GTGGACCTCACCAAGAGCGGAGAGCTCAGAAAAGAGATCAACAGCAACACGGAGCAGCTTCAGTCCAGCA
  AGAGCGAGGTCACCGACCTCAAGCGCATGGTGCAGAACCTGGAGATTGAACTCCAGTCCCAGCTCGCCAT
  GAAGTCGTCCCTGGAAGGCTCGCTGGCTGAAACCGAGGGTGGATACTGCTGCCAGCTATCTCAGATGCAG
  CAGCTTATTGGCAGCCTGGAGGAGCAACTCCAGCAGTTGCGGGCTGATGCAGAGCGCCAGAACGAGGACC
  ACCAGCGGCTGCTGGGCGTCAAGGCTCGCCTGGAGATGGAGATTGAGACCTACCGCCGGCTCCTGGAGGG
  GGATACCCAAGGTGATGGTTTTGATGAAAGCTTATCCCTCACGGTCTCCAAACCTCAGGCACCATCAGTC
  GACTCCTCTAAAGACCCAAATAAAACTCGAAAAATCAAGACAGTCGTGCAGGAGATAGTGAATGGCGAAG
  TGGTCTCATCCCAGGTTCAGGAACTTGAAGAAGCGATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008761
• Insert Size: 1371 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008761.1, NP_001008761.1
• RefSeq Size: 1371 bp
• RefSeq ORF: 1371 bp
• Locus ID: 360625
• UniProt ID: Q6IFW5
• Cytogenetics: 10q31
• Gene Summary: May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity).[UniProtKB/Swiss-Prot Function]