Ctcf (NM_031824) Rat Untagged Clone

CAT#: RN207558

Ctcf (untagged ORF) - Rat CCCTC-binding factor (zinc finger protein) (Ctcf), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ctcf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207558 representing NM_031824
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGTGAGGCGGTTGAAGCCATTGTGGAAGAGTCTGAAACTTTCATTAAAGGAAAGGAAAGAAAGA
  CTTACCAGAGACGCCGGGAAGGGGGCCAGGAAGAAGATGCTTGCCACCTACCCCAGAACCAGACAGACGG
  GGGTGAGGTGGTCCAGGATGTCAACAGTAGCGTACAGATGGTAATGATGGAACAGCTGGACCCTACCCTT
  CTCCAAATGAAGACTGAAGTAATGGAGGGGACAGTGGCTCCTGAAGCAGAAGCTGCAGTGGACGATACCC
  AAATCATAACCCTGCAGGTTGTAAACATGGAGGAACAGCCCATTAATATAGGAGAACTTCAGCTTGTCCA
  AGTACCTGTTCCTGTGACTGTACCTGTTGCTACTACGTCAGTAGAAGAACTTCAGGGGGCTTATGAGAAT
  GAAGTGTCTAAAGAGGGCCTTGCAGAAAGTGAACCGATGATATGTCACACCTTACCTTTACCTGAAGGAT
  TTCAGGTGGTAAAAGTGGGGGCCAATGGAGAAGTGGAGACACTAGAGCAGGGGGAGCTTCCTCCTCAGGA
  AGACCCTAGCTGGCAAAAAGACCCAGACTATCAGCCACCAGCCAAAAAAACAAAGAAAACCAAAAAGAGT
  AAACTTCGTTACACAGAGGAGGGCAAAGACGTGGATGTGTCTGTGTATGATTTTGAGGAAGAACAGCAGG
  AAGGACTGCTGTCTGAGGTTAATGCAGAGAAAGTGGTTGGTAATATGAAGCCTCCGAAGCCAACAAAAAT
  TAAAAAAAAAGGTGTAAAGAAAACATTCCAGTGTGAGCTTTGCAGTTACACGTGTCCACGGCGTTCAAAT
  TTGGATCGTCACATGAAAAGCCACACTGATGAGAGACCACACAAGTGCCATCTCTGTGGCAGAGCATTCA
  GAACAGTAACCCTCCTGAGGAATCATCTTAACACACACACAGGTACTCGTCCTCACAAGTGCCCCGACTG
  CGACATGGCTTTTGTGACCAGTGGAGAGTTGGTGCGGCATCGTCGTTATAAACACACTCATGAGAAACCA
  TTTAAGTGTTCCATGTGTGATTATGCCAGTGTAGAAGTCAGCAAATTAAAACGACACATTCGCTCTCATA
  CTGGAGAGCGCCCGTTCCAGTGCAGTTTGTGCAGTTATGCCAGCAGGGACACATACAAGCTGAAAAGGCA
  TATGAGAACCCATTCAGGTGAAAAACCTTATGAATGTTATATTTGTCATGCTCGGTTTACCCAGAGTGGT
  ACCATGAAGATGCACATTTTACAGAAGCACACAGAAAATGTGGCCAAATTTCATTGTCCCCACTGTGACA
  CTGTCATAGCCCGAAAAAGTGATTTGGGTGTCCACTTGAGAAAGCAGCATTCCTATATTGAGCAGGGCAA
  AAAATGTCGCTACTGTGATGCTGTGTTTCATGAGAGATATGCTCTTATCCAGCATCAAAAATCACACAAA
  AATGAGAAGCGCTTCAAGTGCGACCAGTGTGATTATGCTTGTAGACAGGAGAGGCACATGATCATGCACA
  AGCGCACTCACACGGGGGAGAAGCCTTATGCCTGCAGCCACTGCGACAAGACCTTCCGCCAGAAACAGCT
  CCTTGACATGCATTTCAAGCGCTATCATGATCCCAACTTTGTCCCCGCTGCCTTTGTCTGTTCTAAGTGT
  GGGAAAACATTCACACGCCGGAACACAATGGCGAGACACGCAGATAACTGTGCTGGTCCAGACGGTGTAG
  AAGGGGAAAATGGAGGAGAGACAAAGAAGAGCAAACGAGGAAGAAAAAGAAAGATGCGGTCTAAAAAAGA
  AGACTCCTCTGACAGTGAAAATGCTGAGCCGGATCTCGATGACAATGAGGAGGAGGAGGAGCCTGCTGTA
  GAAATTGAACCTGAGCCAGAGCCGCAGCCGCAGCCTCAGCCACAGCCGCAGCCGCAGCCTGTGGCCCCAG
  CCCCACCACCTGCCAAGAAGAGAAGAGGGCGACCTCCTGGAAGAACCAACCAACCCAAACAGAACCAGCC
  AACAGCCATCATCCAGGTTGAAGACCAGAATACAGGTGCAATTGAGAACATTATAGTTGAAGTCAAAAAA
  GAGCCAGATGCCGAGCCTGCGGAGGGGGAAGAGGAGGAGGCTCAGGCAGCACCCGCAGACGCCCCCAACG
  GAGACCTCACGCCTGAGATGATTCTCAGCATGATGGACCGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031824
• Insert Size: 2214 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031824.1, NP_114012.1
• RefSeq Size: 3708 bp
• RefSeq ORF: 2214 bp
• Locus ID: 83726
• UniProt ID: Q9R1D1
• Cytogenetics: 19q12
• Gene Summary: binds to and protects the H19 differentially methylated domain from de novo methylation during oocyte growth and is involved in normal preimplantation development [RGD, Feb 2006]