Tnf (NM_012675) Rat Untagged Clone

CAT#: RN206710

Tnf (untagged ORF) - Rat tumor necrosis factor (TNF superfamily, member 2) (Tnf), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tnf
• Synonyms: RATTNF; TNF-alpha; Tnfa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206710 representing NM_012675
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACGGAAAGCATGATCCGAGATGTGGAACTGGCAGAGGAGGCGCTCCCCAAAAAGATGGGGGGCC
  TCCAGAACTCCAGGCGGTGTCTGTGCCTCAGCCTCTTCTCATTCCTGCTCGTGGCGGGGGCCACCACGCT
  CTTCTGTCTACTGAACTTCGGGGTGATCGGTCCCAACAAGGAGGAGAAGTTCCCAAATGGGCTCCCTCTC
  ATCAGTTCCATGGCCCAGACCCTCACACTCAGATCATCTTCTCAAAACTCGAGTGACAAGCCCGTAGCCC
  ACGTCGTAGCAAACCACCAAGCGGAGGAGCAGCTGGAGTGGCTGAGCCAGCGTGCCAACGCCCTCCTGGC
  CAATGGCATGGATCTCAAAGACAACCAACTGGTGGTACCAGCAGATGGGCTGTACCTTATCTACTCCCAG
  GTTCTCTTCAAGGGACAAGGCTGCCCCGACTACGTGCTCCTCACCCACACCGTCAGCCGATTTGCCATTT
  CATACCAGGAGAAAGTCAGCCTCCTCTCTGCCATCAAGAGCCCTTGCCCTAAGGACACCCCTGAGGGAGC
  TGAGCTCAAGCCCTGGTATGAGCCCATGTACCTGGGAGGAGTCTTCCAGCTGGAGAAGGGGGACCTGCTC
  AGCGCTGAGGTCAACCTGCCCAAGTACTTAGACATCACGGAGTCCGGGCAGGTCTACTTTGGAGTCATTG
  CTCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012675
• Insert Size: 708 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012675.3, NP_036807.1
• RefSeq Size: 1687 bp
• RefSeq ORF: 708 bp
• Locus ID: 24835
• UniProt ID: P16599
• Cytogenetics: 20p12
• Gene Summary: acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]