Mmp1 (NM_001134530) Rat Untagged Clone

CAT#: RN206608

Mmp1a (untagged ORF) - Rat matrix metallopeptidase 1a (interstitial collagenase) (Mmp1a), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mmp1
• Synonyms: Clgn; MMP-1; Mmp1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206608 representing NM_001134530
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTAGCCTTCCTTTGCTGTTGCTTCTCTTGGCTACCAGCTCATACAGTTTCCCCGTGTTTCAGGACG
  GAGCCCGGCAGAATGTGGAAACAGTCTGGAAATACCTGGAAAACTACTACAACTTGGGTGAAGACGTCCA
  AGCTAAAAACGTGGATGCCAAGGAGGTGATGGCTGAAAAGCTGAGGCAAATGCAGCAGTTATTTGGGCTG
  AAAGTGACAGGAAATTCAAATTCTGAAACCCTGAGTGCTATGAGGAAACCTAGGTGTGGGGTGCCTGATG
  TGGCCCCATATGCCATTACTCACAACAATCCTCGTTGGACCAAAACACATCTGACTTACAGCATTTTAAA
  CTACACACCATATTTGCCAAAAGAAGTTATAGAAAACGCCATCGAGAGAGCCTTTAAAGTCTGGAGTGAT
  GTGACACCACTAACATTCGAAAGGGTTTTTGAGGAGGAAGGTGATATTGTGTTCGCCTTCTACAGAGGAG
  ACCATAGTGACAATAACCCATTTGATGGACCTGAATATGGACTTGCTCACACATTCCCACCAGGCCCACG
  TTTGGGGGGCGATGTTCATTATGATCTGGATGAGACGTGGACCGACAACAGTGACAATTTCAATTTGTTC
  TATGTTACGGCTCATGAACTGGGCCACTCCCTTGGACTCACTCATTCTAGAGATGTAGGAGCACTAATGT
  TCCCCAGCTACACATGGTACACTGAAGACTTTGTGCTAAACCAGGAGGATATTAATCGCATCCAGGCTTT
  ATATGGGCCTTCCCCAAATCCCATCCAGCCAACAGGTGCAACAACACCACATCCATGTGATGGTGATCTA
  ACCTTCGACGCTATAACTACGTTTAGGGGAGAGGTGATGTTCTTTAAAGGCAGGTTCTACATTCGTGTAA
  ATAAGTTCATGCCAGAAGCTGAGCTCAATTTGATAGGTATTATCTGGCCAAATCTGCCAGGTAAACTTGA
  TGCTGCTTATGAAGTTAGTATGATAGATCAAGTCCGCTATTTCAAAGGCAATAAAGTCTGGGTTGTTCGA
  GAGCATACTGTACTGAGAGGATTCCCCACAGACATCCATAGCTTCTTTGGCTTCCCTAGCAGTGTGACAC
  ACATTGACGCTGCCGTTTGTGAGGAAGAGACTGGAAAAACATACTTCTTCGTTGACAACATGTACTGGAG
  GTATGATGAAAATACACAGTCCATGGATCCAGGTTATCCTAGATTAACAGCAGAAGATTTCCCTGGAATT
  GACGATAAAGTTGATGATGTTTTCCAAAAAGGCGAAAATTTCTACTTCTTTCACCAATCAGTTCAACGCA
  GATTTAGCCTCCGAATAAGAAGAGTTGATGACTCCCGTGATTCTAGTACCTGGTTCAACTGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001134530
• Insert Size: 1395 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134530.1, NP_001128002.1
• RefSeq Size: 2021 bp
• RefSeq ORF: 1395 bp
• Locus ID: 300339
• Cytogenetics: 8q11