Crhr1 (NM_030999) Rat Untagged Clone

CAT#: RN206382

Crhr1 (untagged ORF) - Rat corticotropin releasing hormone receptor 1 (Crhr1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Crhr1
• Synonyms: CRFR1; CRH-R 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206382 representing NM_030999
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACGGCGCCCGCAGCTCCGGCTCGTGAAGGCCCTTCTCCTTCTGGGGCTGAACCCTGTGTCCACCT
  CCCTTCAGGATCAGCGCTGTGAGAACCTGTCCCTGACCAGCAATGTTTCTGGCCTGCAGTGCAATGCATC
  CGTGGACCTCATTGGCACCTGCTGGCCCCGGAGCCCTGCGGGCCAGTTGGTGGTCCGACCCTGCCCTGCC
  TTTTTCTACGGTGTCCGCTACAACACGACAAACAATGGCTACCGGGAGTGCCTGGCCAACGGCAGCTGGG
  CAGCCCGTGTGAATTATTCTGAGTGCCAGGAGATTCTCAACGAAGAGAAGAAGAGCAAAGTACACTACCA
  TGTTGCAGTCATCATCAACTACCTGGGTCACTGCATCTCCCTGGTAGCCCTCCTGGTGGCCTTTGTCCTC
  TTCTTGCGGCTCAGGAGCATCCGGTGCCTGAGAAACATCATCCACTGGAACCTCATCTCGGCTTTCATCC
  TACGCAACGCCACGTGGTTTGTGGTCCAGCTCACCGTGAGCCCCGAGGTGCACCAGAGCAATGTGGCCTG
  GTGTAGGTTGGTGACAGCCGCCTACAATTACTTCCATGTAACCAACTTCTTCTGGATGTTCGGTGAGGGC
  TGCTACCTGCACACAGCCATTGTGCTCACGTACTCCACCGACCGTCTGCGCAAGTGGATGTTCGTCTGCA
  TTGGCTGGGGTGTACCTTTCCCCATCATTGTGGCTTGGGCCATTGGGAAGCTGCACTACGACAATGAAAA
  GTGCTGGTTTGGCAAACGTCCTGGGGTATACACTGACTACATCTACCAGGGCCCCATGATCCTGGTCCTG
  CTGATCAACTTTATCTTTCTCTTCAACATTGTCCGCATCCTCATGACCAAACTCCGGGCATCCACTACAT
  CTGAGACCATTCAGTACAGGAAGGCTGTGAAGGCCACTCTGGTGCTCCTGCCCCTTCTGGGCATCACCTA
  CATGTTGTTCTTCGTCAACCCTGGAGAGGACGAGGTCTCCAGGGTCGTCTTCATCTACTTCAACTCTTTT
  CTGGAGTCCTTTCAGGGCTTCTTTGTGTCTGTGTTCTACTGTTTTCTGAACAGTGAGGTCCGCTCCGCTA
  TCCGGAAGAGGTGGCGTCGGTGGCAGGACAAGCACTCCATCAGAGCCCGAGTGGCCCGAGCTATGTCCAT
  CCCCACCTCCCCGACCAGAGTCAGCTTTCACAGCATCAAGCAGTCCACAGCAGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030999
• Insert Size: 1248 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030999.4, NP_112261.1
• RefSeq Size: 2510 bp
• RefSeq ORF: 1248 bp
• Locus ID: 58959
• UniProt ID: P35353
• Cytogenetics: 10q32.1
• Gene Summary: This gene encodes a member of the G-protein coupled receptor family. The encoded protein is a receptor for corticotropin-releasing factor (CRH) and urocortin (UCN). The interaction of this protein with CRH and UCN triggers G-protein coupled signaling. This protein plays a pivotal role in mediating the CRH-elicited effects in depression and anxiety. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.