Notch1 (NM_001105721) Rat Untagged Clone

CAT#: RN206140

Notch1 (untagged ORF) - Rat Notch homolog 1, translocation-associated (Drosophila) (Notch1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Notch1
• Synonyms: NOTCH; TAN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206140 representing NM_001105721
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCGGCTCCTGGCGCCCTTGCTCTGCCTAACGCTGCTGCCCGCGCTCGCCGCAAGAGGCTTGAGAT
  GCTCCCAGCCAAGTGGGACCTGCCTGAATGGAGGGAGGTGCGAAGTGGCCAACGGCACTGAAGCCTGTGT
  CTGCAGCGGAGCGTTCGTGGGCCAGCGATGCCAGGACCCCAGCCCTTGCCTCAGCACACCATGTAAGAAT
  GCTGGAACGTGCTATGTTGTGGACCATGGCGGCATCGTGGACTATGCCTGCAGTTGCCCCCTGGGTTTCT
  CTGGGCCCCTCTGCCTGACACCTCTGGCCAATGCCTGCCTGGCCAACCCCTGCCGCAACGGGGGGACCTG
  TGACCTGCTCACTCTCACAGAATACAAGTGCCGGTGCCCGCCAGGGTGGTCAGGAAAGTCATGTCAGCAA
  GCCGACCCCTGTGCCTCCAACCCCTGTGCCAATGGTGGCCAGTGCCTGCCCTTTGAGTCTTCATACATCT
  GTGGCTGCCCGCCCGGCTTCCATGGCCCCACCTGCAGACAAGATGTTAACGAGTGCAGCCAGAACCCTGG
  GTTGTGCCGTCATGGCGGCACGTGCCACAATGAGATTGGCTCCTATCGCTGTGCCTGCCGTGCCACCCAC
  ACTGGTCCCCACTGCGAGCTGCCCTACGTGCCCTGCAGCCCCTCACCCTGCCAGAACGGAGGCACCTGCC
  GCCCTACGGGGGACACCACCCACGAGTGTGCCTGCCTGCCAGGCTTTGCTGGACAGAACTGTGAAGAAAA
  TGTGGATGACTGCCCAGGAAACAACTGCAAGAACGGGGGTGCCTGTGTGGACGGTGTGAATACCTACAAT
  TGCCGCTGCCCACCGGAGTGGACAGGTCAGTACTGCACAGAGGATGTGGACGAGTGTCAGCTCATGCCCA
  ACGCCTGCCAGAATGGCGGAACCTGCCACAACTCCCACGGTGGCTACAACTGCGTGTGTGTCAATGGCTG
  GACTGGTGAGGACTGCAGTGAGAACATTGATGACTGTGCCAGTGCCGCCTGTTTTCAGGGTGCCACCTGC
  CATGACCGTGTGGCTTCCTTCTACTGCGAGTGTCCACATGGGCGCACAGGCCTGCTGTGCCACCTGAACG
  ATGCGTGTATCAGCAACCCCTGCAACGAGGGCTCCAACTGCGACACCAACCCTGTCAACGGCAAGGCCAT
  CTGCACTTGCCCCTCGGGGTACACGGGGCCAGCCTGCAGCCAGGACGTGGATGAGTGCGCTCTAGGTGCC
  AACCCGTGTGAGCACGCGGGCAAGTGCCTCAACACACTGGGCTCTTTCGAGTGTCAGTGTCTACAGGGCT
  ACACTGGGCCCCGCTGTGAGATTGATGTCAACGAGTGCATCTCCAACCCATGTCAGAATGATGCCACGTG
  CCTGGACCAGATTGGGGAGTTTCAGTGTATATGTATGCCAGGTTATGAGGGTGTATACTGTGAGATCAAC
  ACGGACGAGTGTGCCAGCAGCCCCTGTCTACACAATGGCCGCTGCGTGGACAAGATCAACGAGTTCCTGT
  GTCAGTGTCCCAAAGGCTTCAGCGGGCACCTGTGCCAGTATGACGTGGATGAGTGCGCCAGCACACCATG
  CAAGAACGGCGCCAAGTGCCTGGATGGGCCCAACACCTACACCTGCGTGTGCACAGAAGGTTACACGGGG
  ACCCACTGCGAGGTGGACATTGACGAGTGTGACCCTGACCCCTGTCACTATGGTTTGTGCAAGGATGGTG
  TGGCCACCTTTACCTGCCTCTGCCAGCCAGGCTACACAGGCCATCACTGTGAGACCAACATTAATGAGTG
  TCACAGCCAGCCGTGCCGCCATGGCGGCACCTGCCAGGACCGTGACAACTACTACCTCTGCTTATGCCTC
  AAGGGGACCACAGGACCCAACTGTGAGATCAATCTGGATGACTGTGCGAGCAACCCCTGTGACTCTGGCA
  CGTGTCTGGACAAGATCGATGGCTACGAGTGTGCGTGCGAGCCAGGCTACACAGGGAGCATGTGTAATGT
  CAACATTGACGAATGTGCGGGCAGCCCCTGCCACAACGGGGGCACCTGTGAGGATGGCATCGCCGGCTTC
  ACTTGCCGCTGCCCCGAGGGCTACCACGACCCTACGTGCCTGTCTGAGGTCAACGAGTGCAACAGTAACC
  CCTGCATCCATGGAGCTTGCCGGGATGGCCTCAATGGATACAAATGTGACTGTGCCCCTGGGTGGAGTGG
  GACAAACTGTGACATCAACAACAATGAGTGTGAGTCCAACCCTTGTGTCAACGGTGGCACCTGCAAAGAC
  ATGACCAGTGGCTACGTATGCACCTGCCGAGAAGGCTTCAGTGGCCCTAACTGCCAGACCAACATTAACG
  AATGTGCTTCCAACCCCTGCCTGAACCAGGGCACCTGCATTGATGATGTCGCTGGGTACAAATGCAACTG
  CCCTCTGCCCTATACAGGAGCCACATGTGAGGTGGTGTTGGCCCCATGTGCCACCAGCCCCTGCAAAAAC
  AGTGGGGTATGCAAGGAGTCTGAGGACTATGAGAGCTTTTCCTGTGTCTGTCCCACAGGCTGGCAAGGTC
  AAACCTGCGAGATCGACATCAATGAGTGTGTGAAAAGCCCGTGTCGCCATGGTGCCTCTTGCCAGAACAC
  CAATGGCAGCTACCGCTGCCTCTGCCAGGCTGGCTACACGGGTCGCAACTGCGAGAGTGACATCGATGAC
  TGCCGACCCAACCCATGTCACAACGGGGGTTCCTGCACTGACGGGGTCAACGCGGCCTTCTGCGACTGCC
  TGCCCGGCTTCCAGGGTGCCTTCTGTGAGGAGGACATCAACGAATGCGCCAGCAATCCATGCCAAAATGG
  CGCCAACTGCACTGACTGCGTGGACAGCTACACGTGCACCTGCCCCACGGGCTTCAATGGCATCCATTGC
  GAGAACAACACACCTGACTGTACCGAGAGCTCCTGTTTCAATGGTGGCACCTGTGTGGATGGTATCAACT
  CCTTCACCTGTCTGTGCCCACCTGGCTTCACGGGCAGCTACTGCCAGTATGACGTCAATGAGTGTGACTC
  ACGGCCCTGTCTGCATGGTGGCACCTGCCAAGACAGCTATGGTACCTATAAGTGTACCTGCCCACAGGGC
  TACACTGGTCTCAACTGCCAGAACCTTGTGCGCTGGTGTGACTCAGCTCCCTGCAAGAATGGCGGCAAGT
  GCTGGCAGACCAACACACAGTACCACTGCGAGTGCCGCAGCGGCTGGACTGGCTTCAACTGCGACGTGCT
  CAGTGTGTCCTGCGAGGTGGCTGCACAGAAGCGAGGCATCGATGTCACTCTCCTATGCCAGCACGGAGGG
  CTCTGTGTGGATGAGGAAGACAAGCATTACTGCCACTGCCAGGCAGGATACACGGGCAGCTACTGTGAGG
  ACGAGGTGGACGAGTGCTCACCTAATCCCTGCCAGAACGGAGCCACCTGCACTGACTATCTCGGTGGCTT
  TTCCTGCAAGTGTGTGGCTGGGTACCATGGCTCTAACTGCTCTGAGGAGATCAACGAGTGCCTATCCCAA
  CCCTGCCAGAATGGGGGTACCTGCATTGATCTGACCAACACCTACAAGTGCTCCTGCCCCAGGGGCACAC
  AGGGTGTACACTGTGAGATCAACGTCGATGACTGCCATCCTCCCCTAGACCCTGCTTCCCGAAGCCCCAA
  ATGCTTCAATAATGGCACCTGCGTGGACCAGGTGGGTGGCTATACCTGCACCTGCCCGCCAGGCTTCGTC
  GGGGAGCGGTGCGAGGGCGATGTCAATGAGTGTCTCTCCAACCCCTGTGACCCACGTGGCACCCAGAACT
  GCGTGCAGCGTGTTAATGACTTCCACTGCGAGTGCCGGGCTGGCCACACTGGACGCCGCTGTGAGTCGGT
  CATTAATGGCTGCAGGGGCAAACCATGCAGGAATGGAGGTGTCTGTGCTGTGGCCTCCAACACCGCCCGT
  GGATTCATCTGTAGGTGCCCTGCGGGCTTCGAGGGTGCCACTTGTGAAAATGACGCCCGCACTTGTGGCA
  GTTTGCGCTGCCTCAACGGTGGTACGTGCATCTCAGGCCCACGCAGTCCCACCTGCCTATGCCTGGGCTC
  CTTCACTGGCCCTGAATGCCAGTTCCCAGCCAGCAGCCCCTGTGTGGGTAGCAACCCCTGCTACAATCAG
  GGCACCTGTGAGCCCACATCCGAGAGCCCTTTCTACCGCTGTCTATGCCCTGCCAAATTCAACGGGCTGC
  TGTGCCACATCCTGGACTACAGCTTCACAGGTGGCGCCGGGCGCGACATTCCCCCGCCACAGATTGAGGA
  GGCCTGCGAACTGCCTGAGTGCCAGGAGGATGCAGGCAATAAGGTCTGCAACCTTCAGTGTAATAATCAC
  GCGTGTGGCTGGGACGGCGGCGACTGCTCCCTCAACTTCAATGACCCTTGGAAGAACTGCACTCAGTCCC
  TGCAGTGCTGGAAGTATTTTAGTGACGGCCACTGTGACAGCCAGTGCAACTCAGCTGGCTGCCTTTTTGA
  TGGCTTCGACTGCCAGCTCACCGAGGGACAGTGCAACCCCCTGTATGACCAGTACTGCAAGGACCACTTC
  AGTGATGGCCACTGCGACCAGGGCTGCAACAGTGCCGAGTGTGAGTGGGATGGACTGGACTGTGCAGAGC
  ATGTCCCCGAGCGACTGGCAGCCGGCACACTGGTGCTGGTGGTGCTGCTTCCGCCCGATCAGCTACGCAA
  CAACTCCTTCCACTTCCTGCGGGAGCTCAGCCACGTGCTGCACACCAACGTGGTCTTCAAGCGTGACGCG
  CAGGGCCAGCAGATGATCTTCCCCTACTATGGCCGCGAGGAAGAGCTACGCAAGCACCCGATCAAGCGTT
  CTGCAGTGGGCTGGGCCACAACGTCACTGCTTCCTGGCACCAATGGTGGGCGCCAGCGCAGGGAGCTGGA
  CCCCATGGACATCCATGGCTCCATCGTCTACCTGGAGATCGACAACCGGCAGTGTGTACAGTCATCCTCG
  CAATGCTTCCAGAGCGCCACCGATGTGGCTGCCTTCCTAGGTGCTCTTGCCTCCCTCGGCAGCCTCAATA
  TCCCCTACAAGATCGAAGCCGTAAAGAGTGAGACGGTGGAGCCTCCGCTGCCCTCACAGCTGCACCTCAT
  GTACGTGGCGGCAGCTGCCTTCGTGCTCCTGTTCTTTGTGGGCTGTGGGGTGCTGCTGTCCCGCAAGCGC
  AGGCGGCAGCATGGCCAGCTCTGGTTCCCTGAGGGTTTCAAAGTGTCAGAGGCCAGCAAGAAGAAGCGGA
  GAGAACCCCTCGGCGAGGACTCAGTCGGCCTCAAGCCCCTGAAGAACGCCTCAGATGGTGCCCTGATGGA
  CGACAATCAGAACGAGTGGGGGGACGAAGACCTGGAGACCAAGAAGTTCCGGTTTGAGGAACCAGTGGTT
  CTCCCTGACCTGGATGATCAGACTGACCACCGGCAGTGGACCCAGCAGCACCTGGATGCCGCTGACCTAC
  GTGTGTCTGCCATGGCCCCAACGCCGCCTCAGGGGGAGGTAGATGCTGACTGCATGGACGTCAATGTTCG
  AGGACCAGATGGCTTCACTCCCCTAATGATTGCCTCCTGCAGCGGAGGGGGCCTGGAGACAGGCAACAGT
  GAGGAAGAAGAAGATGCACCTGCTGTCATCTCCGACTTCATCTATCAGGGTGCCAGCTTGCACAACCAGA
  CGGACCGCACAGGGGAGACTGCCCTGCACCTGGCTGCCCGATACTCTCGTTCAGATGCTGCCAAGCGCTT
  GCTGGAGGCCAGCGCAGATGCCAACATCCAAGACAACATGGGGCGTACCCCATTACATGCCGCTGTTTCT
  GCAGACGCTCAGGGTGTCTTCCAGATCCTGCTCCGGAACAGAGCCACAGATCTGGATGCCCGAATGCATG
  ATGGCACAACCCCTCTGATCCTGGCGGCACGCCTGGCCGTGGAAGGCATGCTAGAGGACCTCATCAACTC
  TCACGCTGATGTCAATGCTGTGGATGACCTAGGCAAGTCAGCTCTGCACTGGGCAGCCGCTGTGAACAAT
  GTGGACGCTGCTGTTGTGCTCCTGAAGAACGGAGCCAACAAAGACATGCAGAACAACAAGGAGGAGACTC
  CCCTGTTCCTGGCCGCCCGTGAGGGCAGCTATGAGACTGCCAAAGTGTTGCTGGACCACTTTGCCAACCG
  GGACATCACGGATCACATGGACCGATTGCCACGGGACATTGCACAGGAGCGCATGCACCACGATATCGTG
  CGGCTTTTGGATGAATACAACCTGGTGCGCAGCCCACAGCTGCATGGCACTGCCTTGGGTGGCACACCCA
  CTCTGTCTCCCACACTCTGCTCGCCCAACGGCTACCTGGGCAACCTCAAGTCTGCCACACAGGGCAAGAA
  GGCCCGAAAGCCCAGCACCAAAGGGCTGGCTTGCAGTAGCAAGGAAGCTAAGGACCTCAAGGCCCGGAGG
  AAGAAGTCCCAGGATGGCAAGGGCTGCCTGTTGGACAGCTCAAGCATGCTGTCACCCGTGGACTCCCTCG
  AGTCACCCCATGGCTACTTGTCAGATGTGGCCTCACCACCCCTCCTTCCCTCCCCGTTCCAGCAGTCTCC
  ATCCATGCCTCTCAGCCACCTGCCAGGTATGCCTGACACCCACCTGGGCATCAGCCACTTGAATGTGGCA
  GCCAAGCCCGAGATGGCAGCTCTGGCCGGAGGCAGCCGGTTGGCCTTTGAGCCACCCCCACCACGCCTCT
  CCCACCTGCCTGTAGCCTCCAGTGCCAGCACAGTGCTGAGTACCAATGGCACAGGGGCTATGAATTTCAC
  CGTGGGTGCACCGGCAAGCTTGAATGGCCAGTGTGAGTGGCTTCCCCGGCTCCAGAATGGCATGGTGCCC
  AGCCAGTACAACCCGCTAAGGCCAGGTGTGACTCCGGGCACACTAAGCACACAGGCAGCTGGCCTCCAGC
  ATGGCATGATGGGCCCGATACACAGCAGCCTCTCCACCAATACCTTGTCCCCGATTATCTACCAGGGCCT
  GCCCAACACAAGGCTGGCCACACAGCCCCACCTGGTGCAGACCCAGCAGGTGCAGCCACAGAACTTACAA
  ATCCAGCCTCAGAACCTGCAGCCACCATCGCAGCCACACCTCAGTGTGAGCTCAGCAGCCAATGGGCACC
  TGGGTCGGAGCTTCCTGAGCGGGGAGCCCAGCCAGGCAGACGTACAGCCGCTGGGCCCCAGCAGTCTGCC
  TGTGCACACCATTCTGCCCCAGGAAAGCCAGGCTCTGCCGACATCACTGCCATCCTCCATGGTCCCACCC
  ATGACCACTACCCAGTTCCTGACCCCTCCTTCTCAGCACAGCTACTCATCCTCACCTGTGGACAACACCC
  CCAGCCACCAGCTGCAGGTGCCAGAGCACCCCTTCCTCACCCCATCCCCTGAGTCCCCTGACCAGTGGTC
  CAGCTCCTCCCCGCATTCCAACATCTCTGATTGGTCCGAGGGCATCTCTAGCCCGCCCACGAGCATGCCG
  TCCCAGATCACCCACATTCCAGAGGCATTTAAGTAA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_001105721
• Insert Size: 7596 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105721.1, NP_001099191.1
• RefSeq Size: 9407 bp
• RefSeq ORF: 7596 bp
• Locus ID: 25496
• UniProt ID: Q07008
• Cytogenetics: 3p13
• Gene Summary: transmembrane receptor; involved in cell-cell interactions important for development and pattern formation [RGD, Feb 2006]