Lmnb1 (NM_053905) Rat Untagged Clone

CAT#: RN206117

Lmnb1 (untagged ORF) - Rat lamin B1 (Lmnb1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Lmnb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206117 representing NM_053905
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGCGACCCCCGTGCAGCAGCGGGCGGGCAGCCGCGCCAGCGCCCCCGCCACGCCGTTCAGCC
  CCACGCGCCTGTCGCGGCTGCAGGAGAAAGAGGAGCTGCGCGAGCTCAACGACCGTCTGGCGGTGTATAT
  TGACAAGGTGCGCAGCTTGGAGACGGAGAACAGCGCGCTGCAGCTGCAGGTGACCGAGCGCGAGGAGGTG
  CGCGGCCGCGAGCTCACCGGGCTCAAGGCTCTCTACGAGACCGAGCTGGCCGACGCACGCCGCGCTCTAG
  ACGACACGGCCCGCGAGCGCGCCAAGCTACAGATCGAGCTGGGCAAGTTCAAGGCCGAGCACGACCAGCT
  GCTGCTCAATTATGCTAAGAAGGAGTCTGATCTCAGTGGAGCCCAGATCAAGCTTCGAGAGTATGAGGCC
  GCGCTAAACTCTAAGGATGCAGCACTGGCTACTGCCCTTGGGGACAAAAAGAGTTTAGAGGGAGACTTGG
  AGGATCTGAAAGACCAGATTGCCCAGTTGGAAGCATCCCTATCTGCCGCCAAAAAGCAGTTGGCGGATGA
  GACTTTGCTTAAAGTGGATCTGGAGAACCGTTGTCAGAGCCTTACTGAGGACTTGGAGTTCCGTAAAAAT
  ATGTATGAAGAGGAGATCAATGAGACAAGAAGGAAGCATGAGACGCGCCTGGTGGAGGTGGACTCCGGGC
  GTCAGATTGAGTATGAGTACAAGCTGGCTCAAGCCCTGCACGAGATGAGAGAGCAGCACGACGCGCAGGT
  GAGGCTGTATAAGGAAGAGCTGGAGCAGACCTACCACGCCAAGCTTGAGAATGCCAGGCTGTCATCAGAG
  ATGAACACTTCCACTGTCAACAGTGCCAGGGGAGGAATGATGGAGAGCCGGATGAGGATCGAGAGCCTTT
  CCTCTCAGCTTTCTAACCTGCAGAAAGATTCTAGAGCATGCTTGGAAAGGATTCAAGAATTGGAGGACAT
  GCTTGCTAAAGAGAGAGACAACTCACGCCGCATGCTGTCTGACAAAGAGAGAGAGATGGCGGAAATAAGG
  GACCAGATGCAGCAGCAGCTGAATGATTACGAACAGCTGCTTGACGTGAAGCTGGCCCTGGACATGGAGA
  TCAGCGCCTACAGGAAACTCTTGGAAGGCGAAGAAGAGCGGTTAAAGCTCTCTCCAAGTCCTTCTTCCCG
  AGTGACCGTGTCCAGAGCCTCGTCCAGCCGCAGTGTGCGCACCACCAGAGGAAAGCGGAAGAGAGTTGAT
  GTGGAAGAATCAGAGGCGAGCAGTAGTGTTAGCATTTCCCACTCTGCTTCAGCCACGGGGAATGTGTGCA
  TTGAAGAGATAGATGTTGATGGGAAGTTTATTCGCTTGAAGAACACTTCTGAACAGGATCAACCAATGGG
  AGGCTGGGAGATGATCAGAAAAATTGGAGACACATCAGTCAGTTACAAATATACCTCAAGATATGTGCTG
  AAGGCCGGCCAGACTGTTACAGTATGGGCTGCAAATGCTGGAGTCACAGCCAGTCCTCCAACTGACCTCA
  TCTGGAAGAACCAGAACTCTTGGGGCACTGGCGAGGATGTGAAGGTTGTGTTGAAGAATTCTCAGGGAGA
  GGAGGTTGCTCAGAGAAGCACAGTCTTCAAAACTACCATACCTGAGGAGGAGGAGGAGGAGGAGGAGGAG
  CCCATCGGAGTGCCCCTGGAGGAGGAACGTTTCCACCAGCAGGGAACCCCAAGAGCATCCAATAAAAGCT
  GTGCCATTATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053905
• Insert Size: 1764 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053905.1, NP_446357.1
• RefSeq Size: 2154 bp
• RefSeq ORF: 1764 bp
• Locus ID: 116685
• UniProt ID: P70615
• Cytogenetics: 18q12.1
• Gene Summary: human homolog is a nuclear lamina protein; involved in nuclear stability, chromatin structure and gene expression [RGD, Feb 2006]