Rasgrp1 (NM_019211) Rat Untagged Clone

CAT#: RN205445

Rasgrp1 (untagged ORF) - Rat RAS guanyl releasing protein 1 (calcium and DAG-regulated) (Rasgrp1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Rasgrp1
• Synonyms: Rasgrp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN205445 representing NM_019211
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAACCCTGGGCAAGGCGAGAGAGGCTCCGCGGAAACCTTGCCATGGCTCCAGAGCTGGCCCCAAAG
  GAAGACTAGAGGCCAAATCAACCAACAGTCCTCTCCCTGCCCAGCCCAGCTTGGCCCAGATCACCCAGTT
  CCGAATGATGGTGTCCCTGGGACATCTGGCCAAAGGAGCCAGCCTGGATGATCTTATTGACAGCTGCATT
  CAATCTTTCGATGCAGATGGAAACCTGTGTCGAAGTAACCAGCTGTTACAAGTCATGCTAACCATGCACC
  GAATCATCATCTCCTCGGCCGAGCTGCTGCAAAAACTCATGAATCTATATAAGGACGCCCTGGAAAAGAA
  TTCTCCAGGAATTTGCTTGAAGATCTGCTATTTTGTCAGGTATTGGATAACAGAATTCTGGATCATGTTC
  AAAATGGATGCCAGCTTGACCAGCACCATGGAAGAGTTTCAGGACCTGGTGAAAGCCAATGGTGAGGAGT
  CCCACTGCCACCTCATCGACACGACACAAATTAATTCTCGAGACTGGTCCAGGAAACTGACTCAGAGGAT
  AAAATCAAATACCAGCAAGAAGCGGAAAGTGTCCCTGCTGTTTGACCATCTTGAACCTGAAGAACTGTCT
  GAACACCTCACCTACCTTGAGTTCAAGTCCTTCCGACGGATATCTTTCTCTGATTATCAGAATTACCTTG
  TAAACAGCTGCGTAAAGGAGAACCCCACCATGGAGCGGTCCATTGCCCTGTGCAATGGCATCTCCCAGTG
  GGTACAACTGATGGTTCTCAGCCGTCCCACCCCACAGCTCCGGGCAGAGGTCTTCATCAAGTTCATCCAT
  GTGGCTCAGAAGCTCCACCAGCTACAGAACTTCAACACGCTAATGGCTGTGATCGGGGGACTGTGTCACA
  GCTCCATCTCCAGGCTCAAGGAGACAAGTTCACATGTCCCACATGAGATCAATAAGGTTCTGGGTGAGAT
  GACTGAACTGCTGTCCTCCTGCAGAAACTATGACAACTACAGGCGAGCCTATGGTGAGTGCACCCACTTC
  AAAATCCCCATACTGGGTGTGCACCTCAAGGACCTCATATCCCTATATGAAGCCATGCCTGACTACCTGG
  AAGACGGGAAGGTGAATGTCCAAAAGCTCCTGGCCCTTTACAATCACATCAATGAGTTGGTCCAGCTGCA
  GGACGTGGCCCCACCATTGGATGCCAACAAGGACCTGGTGCACCTGCTGACGTTATCCCTGGATCTATAC
  TACACCGAAGATGAAATCTATGAGCTTTCCTACGCCCGTGAACCAAGGAACCACAGGGCCCCGCCACTGA
  CACCTTCGAAGCCACCAGTTGTAGTGGACTGGGCCTCTGGAGTGTCTCCCAAACCTGACCCGAAGACCAT
  CAGCAAACACGTCCAAAGGATGGTGGATTCTGTCTTTAAGAACTATGATCTCGACCAGGATGGATATATC
  TCTCAGGAGGAGTTTGAAAAGATTGCTGCGAGCTTTCCATTTTCCTTCTGTGTGATGGACAAAGATAGGG
  AGGGCCTCATCAGCAGAGACGAGATCACAGCCTACTTCATGAGGGCCAGCTCCATCTATTCCAAGCTGGG
  CCTAGGCTTTCCACACAACTTTCAAGAGACCACTTACCTGAAGCCCACCTTCTGTGACAACTGTGCTGGC
  TTTCTCTGGGGTGTGATCAAGCAAGGCTATCGCTGTAAAGACTGTGGGATGAACTGCCACAAACAGTGCA
  AAGACCTGGTAGTGTTTGAGTGCAAGAAACGATCCAAGAGCCCGGCGGTATCCACAGAAAACATCAGCTC
  TGTGGTGCCAATGTCCACTCTTTGTCCACTGGGAACCAAAGATCTGCTCCATGCACCCGAAGAAGGATCT
  TTCATTTTCCAGAATGGAGAGGTTGTGGACCACAGTGAGGAGAGCAAGGATAGGACCATCATGCTCTTGG
  GCGTATCCTCACAGAAAATTTCAGTTCGGCTGAAGAGGACTGTTGCCCACAAGACCACCCAGACAGAATC
  ATTTCCTTGGGTTGGCGGCGAGATGCCCCCTGGTCACTTTGTGCTGACTTCTCCAAGAAAGTCAGCACAA
  GGGGCTCTTTATGTGCACAGTCCAGCATCTCCGTGCCCCAGCCCAGCACTGGTCCGGAAGCGGGCATTCG
  TCAAGTGGGAGAACAAAGAGTCCCTTATCAAACCAAAACCAGAGCTTCACCTCAGGCTCCGGACCTACCA
  AGAACTGGAACAGGAGGTAAATACCCTGAGGGCAGATAACGATGCTCTGAAGATCCAGCTGAAGTATGCA
  CAGAAACAAATAGAATCCCTGCAGCTTGGCAAAAGCAATCACGTCTTAGCACAGATGGACCACGGTGATG
  GTACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019211
• Insert Size: 2388 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019211.2, NP_062084.2
• RefSeq Size: 3624 bp
• RefSeq ORF: 2388 bp
• Locus ID: 29434
• UniProt ID: Q9R1K8
• Cytogenetics: 3q35
• Gene Summary: putative guanine nucleotide exchange factor with Ca2+ and diacylglycerol binding domains; may activate Rap protein mediated inhibition of Ras signaling pathways [RGD, Feb 2006]