Asl (NM_021577) Rat Untagged Clone

CAT#: RN205157

Asl (untagged ORF) - Rat argininosuccinate lyase (Asl), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Asl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN205157 representing NM_021577
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCGGAGAGCGGGAAGCTATGGGGTGGCCGATTTGCAGGCTCGGTCGACCCCACCATGGACAAGT
  TCAACTCATCTATCGCCTATGACCGGCATCTGTGGAATGTGGACCTGCAGGGAAGCAAGGCCTACAGCAG
  GGGCCTGGAGAAGGCAGGGCTTCTCACCAAAGCTGAGATGCAGCAGATACTGCAAGGCCTGGACAAGGTG
  GCTGAAGAGTGGGCCCAAGGCATCTTCAAATTGTACCCTAATGATGAAGACATCCACACGGCCAACGAGC
  GGCGCCTGAAGGAACTCATTGGTGAAGCTGCAGGGAAGTTACACACAGGCAGAAGTCGCAATGACCAGGT
  GGTCACGGACCTCAGGCTGTGGATGAGGCAAACCTGCTCAAAACTCTCCACCTTCCTCAAGGTGCTCATT
  GAAGCCATGGTAGACCGGGCAGAGGCGGAGTGTGAAGTCCTCTTCCCTGGGTACACACACTTGCAGAGAG
  CTCAGCCCATCCGCTGGAGCCACTGGATCCTGAGTCACGCCGTTGCGCTGACACGAGATTTAGAGAGACT
  GAAGGAGGTGCAGAAGCGGATCAATGTCCTGCCACTGGGCAGTGGGGCCATTGCAGGCAACCCTCTGGGT
  GTGGACCGGGAGTTCCTCTGTGCAGAACTGAACTTTGGAGCCATTACGCTCAACAGTATGGATGCCACCA
  GCGAGAGAGACTTCGTGGCTGAGTTCCTGTTTTGGGCTTCTCTGTGCATGACCCATCTCAGCAGGATGGC
  AGAAGACCTGATTCTCTACGGTACCAAGGAATTCAACTTTGTGCAGCTCTCCGATGCCTACAGCACCGGA
  AGCAGCTTGATGCCCCAGAAGAAAAACCCAGACAGCTTGGAGCTGATCCGGAGCAAGGCGGGCCGAGTGT
  TTGGACGGTGCGCAGGACTCCTGATGACCCTCAAGGGACTTCCAAGCACCTACAACAAGGACTTACAGGA
  AGACAAGGAGGCTGTGTTTGAAGTGTCTGACACCATGACAGCTGTCCTCCAAGTAGCCACTGGAGTCATC
  TCTACACTGCAGATTCATCGTGAGAACATGGCACAGGCACTCAGCCCTGACATGCTGGCTACCGACCTTG
  CCTACTACCTGGTCCGCAAAGGGATGCCATTCCGCCAGGCCCACGAGGCCTCAGGGAAAGCTGTGGTCGT
  GGCAGAGATGAAAGGGGTGGCTCTCAACCAGCTGTCACTTCAGGAGCTGCAGACCGTCAGTCCCCTGTTC
  TCGAGTGACGTGAATCTCGTGTGGGACTACAGCCACAGCGTGGAGCAGTACACAGCCTTGGGTGGCACAG
  CACAGTCCAGTGTTGAGTGGCAGATCAGCCAGGTGCGGGCCCTGCTGCAGATGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021577
• Insert Size: 1386 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021577.3, NP_067588.2
• RefSeq Size: 2100 bp
• RefSeq ORF: 1386 bp
• Locus ID: 59085
• UniProt ID: P20673
• Cytogenetics: 12q12