Plk2 (NM_031821) Rat Untagged Clone

CAT#: RN203879

Plk2 (untagged ORF) - Rat polo-like kinase 2 (Drosophila) (Plk2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Plk2
• Synonyms: Snk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN203879 representing NM_031821
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCCTGCGGACTATCACCTACCAGCCGGCCGCCGGCACCAAGATGTGCGAGCAGGCGCTGGGCA
  AAGCTTGCGGCGGGGACTCAAAGAAGAAGCGACCGCAGCAGCCTTCTGAAGACGGGCAGTCCCAAGCCCA
  GGTGACCCCGGCGGCCCCACACCACCATCATCACCATTCTCACTCCGGACCCGAGATCTCGCGGATTATA
  GTCGACCCCACGACGGGGAAGCGCTACTGCCGGGGCAAAGTGCTGGGCAAGGGTGGATTTGCAAAGTGTT
  ACGAAATGACAGATCTGACAAACAACAAAGTCTACGCTGCAAAAATTATCCCTCACAGCAGAGTAGCTAA
  ACCTCATCAAAGGGAAAAGATCGACAAAGAAATTGAGCTTCACAGAATCCTGCACCATAAGCATGTAGTG
  CAGTTTTACCACTACTTTGAAGACAAAGAAAACATTTACATCCTCTTGGAGTACTGCAGTAGAAGGTCCA
  TGGCTCATATCTTGAAAGCAAGAAAGGTGTTGACAGAGCCAGAAGTCCGATACTACCTCAGGCAGATTGT
  GTCGGGACTCAAGTATCTTCACGAACAAGAAATCTTGCATAGGGATCTCAAGCTAGGGAACTTTTTTATT
  AATGAGGCCATGGAGCTGAAGGTTGGAGACTTCGGCTTGGCTGCCAGACTGGAACCTTTGGAACACAGAA
  GGAGAACAATATGTGGAACCCCAAATTATCTCTCCCCTGAAGTCCTCAACAAACAAGGACATGGCTGTGA
  ATCAGACATCTGGGCCTTAGGCTGTGTAATGTATACAATGCTGCTAGGAAGACCTCCATTCGAAACAACA
  AATCTGAAAGAAACGTACAGGTGCATAAGAGAAGCAAGATATACAATGCCGTCTTCATTACTGGCCCCCG
  CCAAGCACTTGATAGCCAGCATGCTGTCCAAAAACCCAGAGGACCGCCCCAGTTTGGATGACATCATTCG
  GCATGACTTCTTTCTGCAGGGCTTCACTCCAGACAGACTCTCTTCTAGCTGTTGCCACACAGTTCCAGAC
  TTCCACTTGTCAAGCCCAGCTAAGAATTTCTTTAAGAAAGCTGCTGCCGCTCTTTTTGGTGGCAAAAAGG
  ACAAAGCAAGATATAATGACACACACAATAAAGTGTCTAAGGAAGATGAAGACATCTACAAGCTTCGGCA
  TGATTTGAAGAAAACATCGATAACCCAGCAACCCAGCAAACACAGAACAGATGAGGAGCTCCAGCCTCCT
  CCAACTACCTTTGCCAAATCTGGGACATCCGCAGTGGAAAACAAACAGCAGATTGGAGATGCAATCCGGA
  TGATAGTTAGAGGGACGCTGGGCAGCTGCAGCAGCAGCAGTGAATGCCTTGAGGATAGCACCATGGGAAG
  TGTTGCAGACACAGTGGCAAGAGTCCTTCGAGGGTGTCTAGAAAACATGCCTGAAGCTGACTGTATTCCC
  AAAGAGCAGTTGAGCACGTCCTTTCAGTGGGTCACCAAATGGGTCGACTACTCCAACAAATACGGCTTTG
  GATACCAGCTCTCAGACCACACTGTTGGAGTCCTTTTCAACAACGGCGCTCACATGAGCCTCCTTCCAGA
  CAAAAAGACAGTTCACTATTATGCGGAACTTGGCCAATGCTCTGTTTTCCCAGCAACAGATGCCCCCGAA
  CAATTTATTAGTCAAGTGACGGTGCTGAAATACTTTTCCCACTACATGGAGGAGAACCTCATGGATGGTG
  GCGATCTCCCTAGTGTTACTGACATTCGAAGACCTCGGCTCTACCTCCTTCAGTGGTTAAAGTCTGATAA
  AGCCTTAATGATGCTCTTCAATGACGGCACATTTCAGGTGAATTTCTACCACGATCATACAAAGATAATT
  ATCTGTAACCAAAATGAAGAATACCTTCTCACATACATCAACGAAGACAGGATATCTACAACTTTCAGAC
  TGACGACCCTGTTGATGTCTGGCTGTTCGCTAGAATTGAAACATCGAATGGAATACGCCCTGAACATGCT
  CTTGCAGAGATGTAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031821
• Insert Size: 2049 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031821.1, NP_114009.1
• RefSeq Size: 2781 bp
• RefSeq ORF: 2049 bp
• Locus ID: 83722
• UniProt ID: Q9R012
• Cytogenetics: 2q14
• Gene Summary: binds Ca2+ and integrin-binding protein Cib; may phosphorylate proteins in the dendrites of activated neurons; may play a role in long term synaptic plasticity [RGD, Feb 2006]