Pdia3 (NM_017319) Rat Untagged Clone

CAT#: RN202839

Pdia3 (untagged ORF) - Rat protein disulfide isomerase family A, member 3 (Pdia3), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pdia3
• Synonyms: ER60; ERp57; Grp58
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202839 representing NM_017319
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTTCAGCTGCCTTGCGCTGCTCCCGGGCGTGGCGTTGCTGCTCGCCTCGGCCCTCCTCGCCTCCG
  CCTCAGACGTGTTGGAACTGACGGACGAAAACTTCGAGAGTCGCGTCTCCGACACGGGCTCAGCTGGGCT
  CATGCTAGTCGAGTTCTTCGCCCCATGGTGTGGACATTGCAAGAGGCTTGCCCCTGAGTATGAAGCTGCA
  GCAACCAGATTAAAAGGAATAGTCCCATTAGCAAAGGTGGACTGCACTGCCAACACAAACACCTGTAATA
  AGTATGGCGTCAGCGGCTACCCAACTCTTAAAATATTTAGAGATGGTGAAGAAGCGGGTGCTTATGATGG
  GCCTAGGACTGCCGATGGAATTGTCAGCCACTTGAAGAAACAAGCAGGACCAGCTTCAGTTCCTCTCAGG
  ACTGAGGACGAATTTAAGAAGTTCATTAGTGATAAAGATGCCTCGGTGGTGGGCTTTTTCAGGGATTTAT
  TCAGTGATGGCCACTCCGAGTTCCTAAAAGCAGCCAGCAACTTGAGAGATAACTACCGATTTGCACACAC
  CAACGTTGAATCTCTGGTGAAGGAGTACGATGATAATGGAGAGGGGATTACTATATTTCGTCCATTACAT
  CTGGCTAACAAATTTGAAGACAAAATTGTGGCATATACTGAAAAGAAAATGACCAGTGGCAAAATCAAGA
  AGTTTATTCAGGAAAGCATTTTTGGTCTCTGTCCTCATATGACAGAAGATAATAAAGATTTGATACAAGG
  CAAGGACTTACTCACGGCTTACTATGATGTGGACTATGAAAAGAATACTAAAGGTTCTAACTACTGGAGA
  AACAGGGTCATGATGGTGGCAAAGACATTCCTTGATGCTGGACACAAACTCAACTTTGCTGTAGCTAGCC
  GTAAAACCTTTAGCCATGAATTGTCTGACTTTGGCTTAGAAAGCACTACTGGAGAGATTCCTGTTGTGGC
  TATCAGAACTGCTAAAGGAGAGAAGTTTGTCATGCAGGAGGAGTTCTCGCGGGATGGCAAGGCTCTTGAG
  CGGTTCCTGCAGGAATACTTTGATGGCAACTTGAAGAGATACCTGAAGTCTGAACCTATCCCCGAGACCA
  ACGAAGGACCTGTCAAGGTGGTGGTAGCAGAGAGTTTTGATGACATAGTGAATGCTGAAGACAAGGACGT
  GCTGATCGAGTTTTATGCTCCTTGGTGTGGCCACTGTAAGAACCTGGAACCCAAGTACAAAGAGCTGGGG
  GAGAAACTCAGCAAAGACCCAAATATTGTCATAGCCAAGATGGATGCCACAGCCAATGATGTGCCTTCTC
  CATATGAAGTCAAGGGTTTTCCTACCATCTACTTCTCACCAGCCAACAAGAAGCTAACTCCAAAGAAGTA
  TGAAGGTGGCCGTGAATTAAATGATCTTATCAGCTATCTACAACGAGAAGCTACAAACCCTCCTATAATT
  CAAGAAGAAAAACCTAAGAAGAAGAAGAAGGCACAAGAGGACCTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017319
• Insert Size: 1518 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017319.1, NP_059015.1
• RefSeq Size: 1874 bp
• RefSeq ORF: 1518 bp
• Locus ID: 29468
• UniProt ID: P11598
• Cytogenetics: 3q35
• Gene Summary: interacts with thiazide-sensitive sodium-chloride cotransporter in the kidney and is induced by glucose deprivation [RGD, Feb 2006]