Hk2 (NM_012735) Rat Untagged Clone

CAT#: RN202128

Hk2 (untagged ORF) - Rat hexokinase 2 (Hk2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202128 representing NM_012735
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGCCTCGCATATGATCGCCTGCTTATTCACGGAGCTCAACCAAAACCAAGTGCAGAAGGTTGACC
  AATTTCTCTACCACATGCGTCTCTCAGATGAGACCCTTCTGGAGATTTCTAGGCGGTTCCGGAAGGAGAT
  GGAGAAAGGGCTAGGAGCTACCACGCACCCTACAGCAGCTGTGAAAATGTTGCCTACCTTTGTGAGGTCA
  ACTCCGGATGGGACAGAACATGGGGAGTTCCTGGCTCTGGATCTTGGAGGAACCAACTTCCGTGTGCTCC
  GAGTAAGGGTGACGGACAATGGCCTCCAGAGAGTGGAGATGGAGAACCAGATCTACGCCATCCCTGAGGA
  CATCATGCGGGGCAGTGGAACCCAGCTGTTTGACCACATCGCCGAATGCCTGGCCAACTTCATGGACAAG
  CTACAAATCAAAGAGAAGAAGCTCCCTCTGGGTTTCACCTTCTCGTTCCCCTGCCACCAGACAAAACTGG
  ATGAGAGTTTTTTGGTCTCGTGGACTAAGGGGTTCAAGTCCAGTGGCGTGGAAGGCAGAGATGTGGTGGA
  CCTGATCCGGAAGGCTATCCAGCGCAGAGGGGACTTTGACATTGACATTGTGGCCGTGGTGAATGACACA
  GTTGGGACCATGATGACTTGTGGCTATGATGATCAGAACTGCGAGATTGGTCTCATTGTGGGCACTGGCA
  GCAACGCCTGCTACATGGAGGAAATGCGTCATATTGACATGGTGGAGGGAGATGAGGGGCGCATGTGCAT
  CAACATGGAGTGGGGAGCCTTTGGGGACGACGGTACACTCAATGACATCCGAACCGAGTTTGACCGAGAG
  ATCGACATGGGCTCGCTGAACCCTGGGAAGCAGCTGTTTGAGAAGATGATTAGCGGGATGTACATGGGGG
  AGCTGGTCAGGCTCATCCTGGTGAAGATGGCCAAGGCAGAGCTGTTGTTCCAAGGGAAACTCAGCCCAGA
  ACTCCTTACCACTGGCTCCTTCGAGACCAAAGATGTCTCGGATATTGAAGAGGATAAGGATGGAATCGAG
  AAGGCCTACCAAATCCTGATGCGCCTGGGTCTGAATCCATTGCAGGAGGATTGTGTGGCCACGCACCGAA
  TCTGCCAGATTGTGTCCACGCGCTCGGCCAGTCTGTGCGCAGCCACCCTGGCCGCGGTGCTGTGGCGAAT
  CAAAGAGAACAAGGGCGAGGAGCGACTTCGCTCCACCATCGGTGTCGATGGCTCCGTCTACAAGAAACAT
  CCCCATTTTGCCAAGCGTCTCCATAAGGCAGTGAGGAGGCTGGTGCCCGACTGTGATGTCCGCTTCCTCC
  GCTCTGAGGATGGCAGCGGCAAGGGGGCTGCTATGGTGACGGCGGTGGCTTACCGTCTGGCTGACCAACA
  CCGGGCCCGCCAGAAGACCCTGGAGTCTCTGAAGCTGAGCCACGAGCAGCTTCTGGAGGTTAAGAGAAGA
  ATGAAGGTGGAAATGGAGCAGGGTCTGAGCAAGGAGACGCATGCGGTCGCCCCTGTGAAGATGCTGCCCA
  CTTACGTGTGTGCCACTCCAGATGGCACAGAGAAAGGAGACTTCTTGGCCTTGGATCTTGGAGGAACAAA
  CTTCCGGGTCCTGCTGGTGCGTGTGCGTAATGGCAAGCGGAGGGGCGTGGAGATGCATAACAAGATCTAC
  TCCATCCCACAGGAGGTTATGCATGGCACTGGGGAAGAGCTCTTCGACCACATTGTCCAGTGCATTGCGG
  ACTTCCTGGAGTACATGGGCATGAAGGGCGTGTCCCTGCCTTTGGGTTTCACATTCTCCTTCCCTTGCCA
  GCAGAACAGCCTAGACCAGAGCATCCTCCTCAAGTGGACAAAGGGATTCAAGGCATCTGGCTGCGAGGGT
  GAGGATGTGGTCACCTTGCTGAAGGAAGCGATTCACCGGCGAGAGGAGTTTGACCTGGATGTGGTTGCCG
  TGGTGAATGACACAGTTGGGACTATGATGACTTGTGGCTACGAAGACCCTCACTGTGAAGTTGGCCTCAT
  TGTTGGCACCGGAAGCAACGCCTGCTACATGGAAGAGATGCGTAATGTGGAGCTGGTGGACGGAGAGGAG
  GGACGGATGTGTGTCAACATGGAGTGGGGAGCATTTGGGGACAATGGCTGCCTGGATGACTTGCGGACCG
  TGTTTGATGTTGCTGTGGATGAGCTTTCTCTCAACCCTGGCAAACAGAGGTTCGAGAAGATGATCAGCGG
  CATGTACTTGGGAGAGATTGTGCGCAACATTCTCATCGATTTCACGAAGCGGGGGCTGCTCTTCCGAGGC
  CGCATCTCAGAGCGCCTCAAGACAAGGGGAATCTTTGAAACTAAGTTCCTGTCTCAGATAGAGAGCGACT
  GCCTAGCCCTGCTACAGGTTCGTGCCATCCTGCGCCACCTAGGGCTGGAGAGCACGTGCGATGACAGCAT
  CATCGTGAAGGAGGTGTGCACTGTGGTTGCCCGGCGCGCTGCACAGCTCTGTGGCGCAGGCATGGCCGCC
  GTAGTGGACAAGATAAGAGAGAACCGTGGGCTGGACAACCTCAAAGTGACAGTGGGCGTGGACGGGACTC
  TGTATAAGCTTCATCCTCACTTTGCCAAGGTCATGCATGAGACGGTGAGAGATCTGGCTCCGAAATGTGA
  CGTGTCCTTCCTGGAATCCGAGGACGGCAGTGGGAAGGGAGCAGCTCTCATCACTGCCGTGGCCTGCCGC
  ATCCGGGAGGCTGGGCAGAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012735
• Insert Size: 2754 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012735.2, NP_036867.1
• RefSeq Size: 3633 bp
• RefSeq ORF: 2754 bp
• Locus ID: 25059
• UniProt ID: P27881
• Cytogenetics: 4q34
• Gene Summary: enzyme involved in the phosphorylation of glucose to produce glucose-6-phosphate; may be involved in the increased rate of glycolysis seen in cancer cells [RGD, Feb 2006]