Lifr (NM_031048) Rat Untagged Clone

CAT#: RN202020

Lifr (untagged ORF) - Rat leukemia inhibitory factor receptor alpha (Lifr), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Lifr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202020 representing NM_031048
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCTTTCTCATGGTGGAGACAGCCATCTTGGATGGCGGACAATAAAAGAGGGAGGATGACTCCAA
  GCCTGCCATGGCTCCTGTCGGCTCTGACTCTTCTGCATCTGATGATGCACGTGAATGGTCTTAAGAGAGG
  GGTACAACAAGATCTGAAATGCACAACCAACAATATGCGAGTGTGGGACTGCTCATGGCCAGCACCCCTC
  GGGGTCAGCCCTGGAACTGTTAAAGACATTTGCATTAAAGACAGGCCCCATTCCTGCCATCGACTAGAGA
  CAACAAACGTTAAGATCCCAGCTCTTTCACCCGGTGATCACGAAGTAACAATAAATTACCAAAACGGCTT
  TCAGAGTAAATTCACACTGAATGAAAAAGATGTTTCTTTAGTTCCGGATACCCCTGAGATCTTGAGTTTG
  TCTGCTGACTTCTCGACCTCCACATTACAACTGAAGTGGAATGACAAAGGGTCTGCTCTCCCTTACCCCT
  CCAATGCCACCTGGGAGGTTAAGGTTCTGCAGAATCCGAGGACGGAGCCCGTAGCACTAGTGTCACTCAA
  CACAGTGCTGAGTGGTAAAGATAAAGGTCACCACTGGAACTGGACCTCAGAACTGCCCTTGCAGTGTGCC
  ACTCACTCTGTGAGCATTAGATGGCACATTGACTACCCTCGTTTCTCTGGGTACAAAGAGTGGAGTGAGT
  GGAGCCCGCTGAAGAACATTTCCTGGACGCGTAATACCGAGACTAACGTTTTTCCTCAAGACAAAGTGGT
  ACTTGCAGGCTCAAACATGACAATTTGTTGTATAAGTACAACGAAGGTGCTTTCAGGACAGATTGGAAAT
  ACGTTCCGCCCTCTTATCCATCTTTATGGGGAGACTGTCGCCATCAACATCCTGAACATACCTGTTTCTG
  AAAACAGCGGCTCAAATGTGATTTTCAGCACAGTCGACGATGTGTATGGAACGGTCGTCTTCGCAGGCTA
  TCCACCCGATGTTCCTCAGAAACTGAGCTGTGAGACACATGACTTGAAAGAGATTATATGTAGCTGGAAT
  CCGGGAAGGATAACGGGACTGGTGGGCCCACGCAACACAGAATACACCTTATTTGAAAGCATTTCAGGAA
  AATCGGCAGTGTTCCACAGGTTTGAAGAACTTGCAAATGAAACCTATTGGTTAACCTTGAAAATGGCTCC
  TGACCAAGAAATTCATAACTTCACCCTGACTGCTCGCAATCCACTGGGCCAGACAGAATCAGCGATAGTC
  ATCAACGCAACTGAAAGAGTTGCTCTTCATGTTCCTATTTCATTGAAAGTGAAGGACGTCAATTCAACAG
  TCGTTACACTTTCTTGGTACTTGCCAGGAAATTTTACAAAGATTAATCTTGTATGTCAGATTGAAATTTG
  TAAAGCTAATTCCAAGAAAGAAGTGCGGAATGTCACAATGAGAGGAGCTGAGGATTCAACCTACCATGTT
  GCTGTGGACAAACTAAACCCATATACTATCTATACTTTCCGGGTTCGTTGTTCTTCCGAAACTTTCTGGA
  AATGGAGCAAGTGGAGTAATGAAAAGCGATATTTAACCACTGAAGCCACTCCTTCAAAGGGACCAGATAC
  TTGGCGAGAGTGGAGTTCTGATGGGAAAAACTTAATCATCTACTGGAAGCCTTTACCCATCAATGAAGCT
  AATGGAAAAATACTTTCCTACAATGTGTCCTGTTCATCAAGCGAGGAGACACAGTCCCTTTCCGAGATCC
  TCGATCCACAACACAAAGCAGAGATAAAGGTTAATAAAAATGACTACATCATCAGTGTGGTGGCAAGAAA
  TTCTGCCGGTTCATCTCCACCTTCAAAAATAGCCAGTATGGAAATCCCAAATGATGACATCACGGTAGAG
  CAAGCGGTTGGAATAGGAAACAGGATCTTCCTCAGCTGGCAGCACAACCCCAACATGACCTGTGACTACG
  TGATTAAATGGTGCAACTCGTCATGGTCTGAGCCCTGCCTCCTGGACTGGATAAAGGTTCCCTCAAACAG
  CACGGGGACTGTCATCGAGTCTGATCAGTTTCAGCCAGGAGTGAGGTATAACTTTTACCTCTATGGGTGT
  ACTAACCAGGGATATCAACTGTTACGTTCTACAATTGGATACATAGAAGAATTAGCTCCAATTGTCGCAC
  CAAACTTTACTGTGGAAGACACATCTGCAGATTCGATATTAGTGAAGTGGGACGACATCCCCGTGGAGGA
  GCTCCGAGGCTTCCTTCGAGGGTATTTATTCTACTTCCAGAAAGGAGAGAGAGACACACCCAAGACACGG
  AGCTTAGAGACAAGTCACTCTGACATCAAGCTAAAGAATATCACTGACATATCCCAGAAGACACTGCGGA
  TTGCCGACCTTCAGGGTAAAACCAGTTACCACCTGGTCCTGCGGGCTTATACACATGGTGGACTAGGCCC
  AGAGAAGAGCATGTTTGTGGTGACCAAGGAGAACTCCGTGGGATTGATTATTGCCATCCTCATCCCAGTG
  GCTGTGGCTGTCATTGTTGGCGTGGTAACAAGCATCCTCTGCTATCGGAAGCGAGAATGGATTAAAGAAA
  CATTCTACCCTGATATTCCCAATCCAGAAAACTGTAAGGCGTTACAGTTTCAGAAGAGCGTCTGTGAGGG
  AAGCAACGCTCTTAAGACATTGGAAATGAATCCATGCACCCCAAATCACGTCGAAGTCCTGGAATCTCGG
  TCAATACCCCCTAAAATAGAAGACACAGAAATAACGTCCCCAGTTTCTGAGCGTCCTGGCGAAAGCTCTG
  AGACAGACCCCGAGAACCAGGCGGCCGTGTCTTACTGCCCACCCATCATTGAGGAGGAAATAACAAACCC
  AGCAGCAGATGAGGCGGGAGGGGCTTCCCAGGTCGTGTACATTGATGTGCAGTCCATGTATCAGCCACAA
  GCCAAAGCAGAGGACGAGCAGGACACTGACCCCGTGATGGTGGCAGGCTATAAGCCACAGATGCGCCTCC
  CCATTAACCCCACAGCGGAAGACACAACAGCAGAAGATGAAGCGGACAAGACCGCAGGTTACAGACCTCA
  GGCCAACGTAAATACTTGGAATTTGGTCTCCCCAGATTCCCCAAGGTCCACAGACAGCAACAGTGAAGTT
  GTCTCATTTGGAAGTCCGTGCTCCATCAATTCCAGGCAATTTTTGATTCCTCCTAAGGATGAAGACTCTC
  CTAAATCTAATGGAGGAGGGTGGTCCTTTACAAACTTCTTCCAGAACAAACCAAATGACTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_031048
• Insert Size: 3282 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031048.1, NP_112310.1
• RefSeq Size: 3407 bp
• RefSeq ORF: 3282 bp
• Locus ID: 81680
• UniProt ID: O70535
• Cytogenetics: 2q16
• Gene Summary: alpha chain of leukemia inhibitory factor glycoprotein and growth factor [RGD, Feb 2006]