Apex1 (NM_024148) Rat Untagged Clone

CAT#: RN200599

Apex1 (untagged ORF) - Rat APEX nuclease (multifunctional DNA repair enzyme) 1 (Apex1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Apex1
• Synonyms: APE; Apex; REF-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200599 representing NM_024148
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAAGCGGGGGAAGAGAGCGGCAGCGGAAGACGGGGAAGAACCCAAGTCCGAGCCAGAGACCAAGA
  AGAGTAAGGGGGCAGCAAAGAAAACTGAGAAGGAGGCCGCAGGAGAGGGCCCTGTCCTGTATGAGGACCC
  TCCAGATCAGAAAACGTCAGCCAGTGGCAAATCTGCCACACTCAAGATATGCTCCTGGAATGTGGATGGG
  CTTCGAGCCTGGATTAAAAAGAAAGGCTTGGATTGGGTAAAGGAAGAAGCACCAGACATCTTGTGCCTCC
  AAGAGACCAAATGCTCAGAGAACAAACTCCCGGCTGAACTGCAAGAGCTGCCTGGACTCACCCATCAGTA
  CTGGTCAGCTCCATCAGACAAAGAAGGATATAGTGGTGTGGGCCTACTTTCCCGCCAATGCCCGCTCAAA
  GTCTCTTATGGCATTGGTGAGGAAGAACATGATCAAGAAGGCCGGGTGATTGTGGCTGAATTTGAGTCCT
  TTATCTTGGTAACAGCCTATGTTCCGAACGCAGGAAGGGGTCTGGTAAGACTGGAGTACCGACAGCGATG
  GGATGAAGCCTTCAGAAAGTTTCTAAAGGACTTGGCTTCCCGGAAACCTCTTGTGCTGTGTGGGGATCTC
  AATGTGGCTCATGAAGAAATAGACCTTCGTAACCCCAAAGGAAACAAAAAGAATGCTGGTTTTACTCCCC
  AGGAGCGCCAAGGCTTTGGGGAAATGCTACAGGCTGTACCACTGGCTGACAGCTTCCGGCATCTCTACCC
  CAACACTGCCTACGCTTATACTTTCTGGACTTACATGATGAATGCCCGCTCTAAGAATGTTGGTTGGCGC
  CTTGATTACTTTTTGCTGTCTCACTCTCTTTTACCTGCTTTGTGCGACAGCAAGATCCGGTCCAAGGCTC
  TTGGCAGTGACCACTGTCCCATCACCCTTTACCTAGCACTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_024148
• Insert Size: 954 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024148.1, NP_077062.1
• RefSeq Size: 1213 bp
• RefSeq ORF: 954 bp
• Locus ID: 79116
• UniProt ID: P43138
• Cytogenetics: 15p14
• Gene Summary: may play roles in DNA repair and in redox homeostasis; regulates Pax8 transcription [RGD, Feb 2006]