Acly (NM_001111095) Rat Untagged Clone

CAT#: RN200532

Acly (untagged ORF) - Rat ATP citrate lyase (Acly), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Acly
• Synonyms: ACL; Clatp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200532 representing NM_001111095
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTTTACAAGTACATCTGTACCACCTCAG
  CCATCCAGAACCGGTTCAAGTATGCCCGGGTTACTCCCGACACAGACTGGGCCCATCTCCTGCAGGACCA
  CCCCTGGCTGCTTAGCCAGAGCTTGGTAGTCAAGCCGGACCAGCTGATCAAACGTCGAGGAAAGCTTGGT
  CTAGTCGGGGTCAACCTCTCTCTGGATGGAGTCAAATCCTGGCTGAAACCTCGACTGGGACATGAGGCCA
  CCGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTGATTGAGCCCTTCGTCCCCCACAGTCAGGCGGA
  GGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTG
  GATGTGGGCGATGTGGACACCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAGAAACTGAACGCTGAAG
  ACATTAAGAGACACCTGTTGGTCCACGCCCCCGAAGACAAGAAAGAAATCCTGGCCAGCTTCATCTCCGG
  CCTATTCAATTTCTACGAAGATCTTTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGAT
  GGTGTCTACATCCTTGACCTGGCGGCCAAGGTGGACGCCACTGCTGACTACATCTGCAAAGTCAAGTGGG
  GTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCATACCCAGAGGAAGCCTACATTGCAGACCTGGA
  TGCCAAAAGTGGGGCGAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCC
  GGGGGTGGCGCCTCTGTCGTGTACAGTGATACCATCTGTGATCTTGGAGGTGTCAACGAACTGGCGAATT
  ACGGGGAGTACTCTGGTGCCCCCAGTGAACAACAGACCTATGACTACGCCAAGACCATCCTCTCACTTAT
  GACTCGAGAGAAGCACCCGGATGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAACGTG
  GCCGCCACCTTCAAGGGCATTGTGAGAGCAATTCGAGATTACCAGGGTCCCCTGAAGGAGCACGAGGTCA
  CCATCTTTGTTCGAAGAGGTGGCCCGAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGGAAGAC
  CACTGGAATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGC
  CACCGGCCCATTCCCAACCAGCCACCCACAGCGGCTCACACTGCCAACTTCCTCCTTAATGCCAGTGGGA
  GCACATCGACACCAGCACCCAGCAGGACAGCGTCTTTTTCCGAGTCCAGAGCTGACGAGGTGGCCCCTGC
  AAAGAAAGCCAAGCCAGCCATGCCCCAAGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCTATC
  GTGTGGGGCATGCAGACCCGGGCTGTGCAAGGCATGCTGGACTTTGACTACGTGTGCTCCCGAGATGAGC
  CTTCAGTGGCTGCTATGGTCTACCCGTTCACGGGGGATCATAAGCAGAAGTTTTACTGGGGACACAAGGA
  AATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAAAAGCATCCGGAGGTAGACGTGCTGATC
  AACTTTGCATCTCTGCGATCGGCTTATGACAGCACCATGGAGACCATGAACTATGCACAGATCCGGACCA
  TAGCCATCATAGCAGAAGGCATCCCTGAGGCTCTCACACGGAAGCTCATCAAGAAGGCAGACCAGAAGGG
  CGTGACCATCATTGGGCCAGCCACGGTTGGGGGCATCAAGCCTGGATGCTTTAAGATTGGGAATACTGGT
  GGGATGCTGGACAACATCCTGGCCTCCAAACTGTATCGCCCAGGCAGTGTGGCCTACGTCTCGCGTTCAG
  GAGGCATGTCTAACGAACTCAATAATATCATCTCTCGGACCACAGATGGTGTCTACGAGGGTGTTGCCAT
  CGGCGGGGACAGGTACCCTGGGTCCACATTCATGGATCACGTGCTGCGTTACCAAGACACTCCAGGAGTC
  AAGATGATTGTAGTTCTTGGGGAGATAGGGGGTACAGAAGAATATAAGATCTGCCGGGGCATCAAGGAGG
  GCCGCCTCACCAAGCCAGTGGTCTGCTGGTGCATCGGGACCTGTGCCACCATGTTCTCTTCTGAGGTCCA
  GTTTGGCCACGCTGGGGCTTGTGCCAACCAGGCTTCTGAAACGGCAGTAGCCAAGAACCAGGCCTTGAAG
  GAAGCGGGAGTGTTTGTGCCCCGAAGCTTTGATGAGCTCGGAGAAATCATTCAGTCCGTGTATGAAGATC
  TTGTGGCCAAAGGCGCCATTGTACCTGCTCAGGAAGTGCCACCTCCAACAGTACCCATGGACTACTCTTG
  GGCCAGGGAGCTGGGTTTAATCCGAAAACCTGCCTCATTCATGACCAGCATCTGTGACGAGCGGGGGCAG
  GAACTCATTTATGCGGGCATGCCCATCACCGAGGTCTTCAAGGAAGAGATGGGCATTGGTGGTGTCCTGG
  GCCTCCTCTGGTTCCAGAGAAGGTTGCCCAAGTATTCCTGCCAGTTCATTGAGATGTGTCTCATGGTCAC
  CGCTGATCACGGGCCAGCTGTCTCCGGGGCCCATAACACTATCATCTGTGCTCGGGCTGGGAAGGACCTG
  GTCTCCAGCCTCACCTCAGGGCTGCTCACCATTGGGGACCGGTTTGGGGGTGCCTTGGACGCAGCAGCGA
  AGATGTTCAGTAAAGCCTTTGACAGCGGCATTATTCCCATGGAGTTTGTGAACAAGATGAAGAAGGAGGG
  GAAACTGATCATGGGCATCGGCCATCGAGTCAAATCGATAAACAACCCAGACATGCGAGTGCAGATCCTC
  AAAGACTTTGTCAAACAGCACTTCCCCGCCACCCCGCTGCTCGACTATGCACTGGAAGTGGAGAAAATCA
  CCACCTCAAAGAAGCCAAATCTTATCCTGAACGTGGATGGTTTCATCGGCGTTGCGTTTGTGGACATGCT
  TAGGAACTGTGGCTCCTTCACCCGGGAGGAAGCTGACGAGTATGTTGACATTGGAGCCCTCAATGGCGTC
  TTTGTGCTGGGAAGGAGTATGGGCTTCATCGGGCACTATCTTGACCAGAAGAGGCTGAAGCAAGGGCTGT
  ATCGTCACCCCTGGGACGACATTTCCTATGTTCTCCCGGAACACATGAGCATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001111095
• Insert Size: 3276 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001111095.1, NP_001104565.1
• RefSeq Size: 4301 bp
• RefSeq ORF: 3276 bp
• Locus ID: 24159
• UniProt ID: P16638
• Cytogenetics: 10q31
• Gene Summary: catalyzes the synthesis of cytosolic acetyl-CoA; plays a role in lipid and cholesterol metabolism [RGD, Feb 2006]
  Transcript Variant: This variant (2) lacks an alternate exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.