Gnas (NM_001024823) Rat Untagged Clone

CAT#: RN200464

Gnas (untagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gnas
• Synonyms: ALEX; G-alpha-8; Gnas1; Gnpas; Nesp55; SCG6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200464 representing NM_001024823
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCATCCCCGACGAGACTGACAGTGAGGTCTGTGGACCCCCTGAAGACTCCAAATCTGACATCCA
  GAGCCCCAGTCAGGCCTTCGAGGAAGTCCAAGTGGGCGGAGACTACAGCCCACCTCCAGAGGAAGCCATG
  CCATTCGAGATCCAACAGCCCAGCCTGGGAGATTTCTGGCCCACCCTGGAGCAGCCTGGACCATCTGGGA
  CCCCATCAGGCATCAAAGCCTTCAACCCAGCGATTTTGGAGCCCGGGACCCCCACTGGTGCACACCCAGG
  CCTGGGAGCCTATAGCCCCCCACCAGAAGAAGCTATGCCATTTGAGTTCAATGAGCCTGCCCAGGAAGAC
  CGTTGCCAGCCTCCCTTGCAAGTCCCAGACCTTGCGCCAGGGGGTCCGGAAGCATGGGTCTCCAGAGCTC
  TTCCCGCGGAGCCCGGGAACCTCGGATTTGAAAACACTGGCTTCCGAGAAGACTACAGCCCTCCACCTGA
  AGAATCTGTGCCATTTCAGCTCGATGGAGAAGAATTCGGGGGCGATAGCCCACCCCCAGGACTCCCGCGA
  GTCACGCCACAAATCGGCATTGGCGGCGAGTTCCCGACAGTCGCGGTCCCGAGTACGCTCTGCCTCGCTC
  CCGCCGCGAACGCGCCTCCCCTCTGGGTCCAGGGCGCCATTGGCAGACCATTCCGCGAGGCTGTCAGATC
  TCCTAACTTCGCATACGACATTTCCCCAATGGAGATCACCAGACCCCTGCTTGAGATTGGCAGAGCCTCC
  ACTGGGGTCGACGACGACACCGCTGTCAATATGGACAGCCCCCCAATCGCAAGTGATGGCCCGCCCATCG
  AAGTCTCGGGAGCCCCAGTTAAGAGCGAGCACGCAAAGAGACCCCCACTTGAGCGACAAGCAGCCGAGAC
  AGGAAACAGCCCTATCAGCAGCACCACTGCAGAGGAGGCAAAAGTCCCCTCCCTCGAGCGAGGAGAAGGA
  TCCCCCACCCAACCTGAAACAGTGCATATCAAGCCAGCTCCTGTTGCGGAGTCCGGAACGGACTCTTCCA
  AAGCCGATCCGGACTCAGCTACACACGCAGTTCTTCAGATCGGTCCTGAGGAAGTCGGAGGAGTCCCAAC
  CATGCCCACCGATCTTCCGCCTGCTTCTGAAGATGCGGGCCCAGATGTCCGGGCAGAACCAGACGGAGGG
  ACAGCCCCAGCCACCCCTGCCGAGTCCGAAGACAACAGAGAACCAGCCGCCGCCGCCGCCGCCGAGCCAG
  CCGCCGAGCCAGCCGCTGAGCCAGCCGCCGAGCCAGCCGCCGAGCCAGCCGCCGAGCCAGCTGCCGAGGC
  AGTCCCTGACACCGAAGCCGAGTCTGCCTCCGGGGCAGTCCCCGACACCCAAGAGGAGCCCGCAGCCGCG
  GCAGCCTCTGCCACGCCGGCGGAGCCTGCCGCCCGGGCAGCCCCCGTCACCCCTACGGAGCCCGCTACCC
  GGGCTGTCCCTTCTGCCAGAGCCCATCCAGCCGCCGGGGCTGTCCCTGGAGCCTCAGCGATGTCAGCCGC
  TGCTAGGGCAGCCGCCGCTAGAGCAGCCTATGCAGGTCCTCTGGTCTGGGGAGCCAGGTCACTCTCGGCT
  ACTCCAGCCGCTCGGGCATCCCTCCCTGCCCGCGCAGCAGCTGCCGCCCGAGCAGCCTCTGCTGCCCGCG
  CAGTCGCTGCCGGCCGGTCAGCCTCTGCCGCCCCAAGCAGGGCCCATCTTAGACCCCCCAGCCCGGAGAT
  CCAGGTTGCTGACCCGCCTACTCCGCGGCCTGCTCCGCGGCCGAGTGCCTGGCCTGACAAATACGAACGT
  GGCCGAAGCTGCTGCAGGTATGAGGCTGCGTCCGGCATCTGCGAGATCGAGTCCTCCAGCGATGAGTCGG
  AAGAAGGGGCCACTGGCTGCTTCCAGTGGCTTCTGCGGCGAAACCGCCGCCCTGGCCAGCCCCGGAGCCA
  CACAGTCGGGAGCAACCCGGTCCGCAACTTCTTCGCCCGAGCCTTCGGAAGCTGCTTCGGTCTATCTGAG
  TGTACCCGATCACGATCCCTCAGCCCCGGGAAGGCCAAGGATCCTATGGAAGAGAGGCGCAAACAGATGC
  GCAAAGAAGCCATGGAGATGCGAGAGCAGAAGCGCGCAGATAAGAAACGCAGCAAGCTCATCGACAAGCA
  ACTGGAGGAGGAGAAGATGGACTACATGTGTACACACCGCCTGCTGCTTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024823
• Insert Size: 2223 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024823.4, NP_001019994.4
• RefSeq Size: 3826 bp
• RefSeq ORF: 2223 bp
• Locus ID: 24896
• Cytogenetics: 3q43
• Gene Summary: This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009]
  Transcript Variant: This variant (1, also known as GNASXL) is paternally expressed. It includes an alternate 5' exon, as compared to variant 3. This variant includes two overlapping open reading frames encoding XLas and Alex, respectively. This RefSeq represents Alex, also known as alexX, which has no similarity to other isoforms encoded by this gene. It interacts with XLas, and their interaction is essential for G-protein signaling in neuroendocrine cells. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.