Dmd (NM_001005244) Rat Untagged Clone

CAT#: RN200462

Dmd (untagged ORF) - Rat dystrophin, muscular dystrophy (Dmd), transcript variant Dp71ab, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dmd
• Synonyms: DNADMD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200462 representing NM_001005244
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGAACACCTCAAAGGCCACGAGACCCAAACAACTTGTTGGGACCATCCCAAAATGACAGAGCTCT
  ACCAGTCTTTAGCTGACCTGAATAATGTCAGGTTCTCCGCTTATAGGACTGCCATGAAGCTCAGAAGACT
  CCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGACGCCTTGGACCAGCACAACCTC
  AAGCAAAATGATCAGCCCATGGATATCCTGCAGATAATTAACTGTTTGACTACTATTTATGACCGTCTGG
  AGCAAGAGCACAACAATTTGGTCAATGTCCCTCTCTGTGTGGATATGTGTCTCAACTGGCTTCTCAATGT
  TTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCTCTGTGTAAA
  GCACACTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACCGGCTTTTGTGACCAGC
  GTAGACTGGGTCTTCTTCTGCATGATTCTATTCAAATCCCAAGACAGTTGGGTGAAGTTGCCTCCTTTGG
  GGGCAGTAACATTGAGCCGAGTGTCAGGAGCTGTTTCCAGTTTGCCAATAATAAACCTGAGATTGAAGCC
  GCTCTCTTCCTTGACTGGATGCGTCTGGAACCCCAGTCTATGGTGTGGCTGCCGGTCTTGCACAGAGTGG
  CTGCTGCTGAAACAGCCAAGCATCAGGCCAAGTGTAACATCTGTAAGGAGTGTCCAATCATTGGATTCAG
  GTACAGAAGCCTGAAACACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGCCGAGTTGCTAAG
  GGCCATAAAATGCACTACCCCATGGTAGAGTACTGCACTCCGACTACATCTGGAGAAGATGTTCGTGACT
  TTGCCAAGGTACTGAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCT
  TCCAGTGCAGACTGTGTTAGAGGGGGACAACATGGAAACGCCTGCCTCGTCCCCCCAGCTTTCACACGAT
  GATACTCATTCACGCATTGAGCATTATGCTAGCAGGCTAGCAGAAATGGAAAACAGCAATGGATCTTATC
  TAAATGATAGCATCTCTCCTAATGAGAGCATAGATGATGAACATTTGTTAATCCAGCATTACTGCCAAAG
  TTTGAACCAGGACTCCCCCCTGAGCCAGCCTCGTAGTCCTGCCCAGATCTTGATTTCCTTAGAGAGTGAG
  GAAAGAGGGGAGCTAGAGAGAATCCTAGCAGATCTTGAGGAAGAAAACAGAAATCTGCAAGCAGAATATG
  ATCGTCTGAAGCAGCAGCATGAGCATAAAGGCCTGTCCCCACTGCCGTCTCCTCCTGAGATGATGCCCAC
  CTCTCCCCAGAGTCCCAGGGATGCTGAACTCATTGCTGAGGCCAAGCTACTGCGCCAACACAAAGGACGT
  CTGGAAGCCAGGATGCAAATCCTGGAAGACCACAATAAACAACTGGAGTCTCAGTTACATAGACTGAGGC
  AGCTCCTGGAGCAACCCCAGGCTGAGGCCAAGGTGAATGGCACCACGGTGTCCTCTCCTTCTACCTCGCT
  GCAGAGGTCAGATAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCAGAATCTATGGGT
  GAGGAAGATCTTCTGAGTCCTCCCCAGGACACAAGCACAGGGTTAGAAGAAGTGATGGAGCAACTCAACA
  ACTCCTTCCCTAGTTCAAGAGGACACAATGTAGGAAGCCTTTTCCACATGGCAGATGATTTGGGCAGAGC
  GATGGAGTCCTTAGTTTCAGTCATGATAGATGAAGAAGGAGCAGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005244
• Insert Size: 1869 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005244.2, NP_001005244.1
• RefSeq Size: 1990 bp
• RefSeq ORF: 1869 bp
• Locus ID: 24907
• Cytogenetics: Xq21
• Gene Summary: a vital component of a muscle sarcolemma membrane-spanning complex that connects cytoskeleton to basal lamina; plays a role in retinal neurotransmission; mutations cause Duchenne muscular dystrophy [RGD, Feb 2006]
  Transcript Variant: This variant (Dp71ab) lacks an exon in the 3' coding region, resulting in a frameshift, as compared to variant Dp71a. The encoded isoform Dp71ab has a longer and distinct C-terminus, as compared to isoform Dp71a.