Sp7 (NM_181374) Rat Untagged Clone

CAT#: RN200117

Sp7 (untagged ORF) - Rat Sp7 transcription factor (Sp7), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Sp7
• Synonyms: Osx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200117 representing NM_181374
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCTCTCTGCTTGAGGAAGAAGCTCACTATGGCTCCAGTCCCCTGGCCATGCTGACAGCAGCCT
  GCAGCAAGTTTGGTGGCTCCAGCCCTCTGCGAGACTCAACAGCCCTGGGAAAAGGAGGCACAAAGAAGCC
  ATACACTGACCTTTCAGCCCCCAAAACCATGGGGGACGCCTACCCAGCTCCCTTCTCAAGCACCAATGGA
  CTCCTCTCCCCTGCAGGCAGTCCCCCGGCCCCGGCCTCTGGCTATGCCAATGACTACCCACCCTTTCCCC
  ACTCATTTCCTGGGCCCACTGGTGCCCAAGACCCGGGGCTGCTGGTGCCGAAGGGGCACAGCTCTTCTGA
  CTGCCTGCCTAGTGTCTACACGTCCTTGGATATGTCCCATCCCTACGGCTCCTGGTACAAGGCGGGCATC
  CATGCAGGCATCTCACCAGGTCCTGGCAACACTCCTACCCCTTGGTGGGACATGCACCCTGGGGGCAATT
  GGTTAGGTGGTGGGCAGGGCCAGGGTGATGGGCTGCAAGGGACACTGTCCACAGGCCCTGCCCAGCCTCC
  ACTGAACCCCCAGCTGCCTACTTACCCGTCTGACTTTGCCCCCCTTAACCCAGCTCCCTATCCAGCACCC
  CACCTCTTGCAACCAGGTCCCCAGCATGTCCTACCCCAAGATGTCTATAAGCCCAAGGCAGTTGGCAATA
  GTGGGCAACTGGAGGGGAGTGGTGCAGGCAAACCTCCCCGGGGTGCAGGCACAGGGGGCAGTGGTGGATA
  TGCAGGCAGTGGGGCAGGGCGTTCTACCTGCGACTGCCCCAACTGTCAGGAGCTAGAGCGGCTGGGGGCA
  GCAGCGGCTGGGCTGAGGAAGAAGCCCATTCACAGCTGCCACATCCCCGGCTGCGGCAAGGTGTACGGCA
  AGGCTTCGCATCTGAAAGCCCACTTGCGCTGGCACACTGGCGAGAGGCCTTTCGTCTGCAACTGGCTTTT
  CTGTGGCAAGAGGTTCACCCGCTCTGACGAGCTGGAGCGCCACGTGCGCACTCACACCCGGGAGAAGAAG
  TTCACCTGTCTGCTCTGCTCCAAGCGCTTTACCCGAAGCGACCACTTGAGCAAACATCAGCGCACCCACG
  GGGAGCCAGGTCCCGGGCCGCCCCCAAGTGGCCCTAAGGAGCTGGGGGAGGGTCGCAGCGTCGGGGAAGA
  AGAAGCCAATCAGCCGCCCCGATCTTCCACCTCGCCTGCACCCCCAGAAAAAGCCCCTGGAGGCAGCCCA
  GAGCAGAGCAACCTGCTAGAGATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181374
• Insert Size: 1287 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181374.2, NP_852039.2
• RefSeq Size: 1385 bp
• RefSeq ORF: 1287 bp
• Locus ID: 300260
• Cytogenetics: 7q36
• Gene Summary: may act as a transcription factor; mouse homolog is required for bone formation and osteoblast differentiation [RGD, Feb 2006]