TAF1 (NM_001286074) Human Tagged ORF Clone

CAT#: RG240220

TAF1 (tGFP-tagged) - Human TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 3

Product Images

Circular map for RG240220

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TAF1
• Synonyms: BA2R; CCG1; CCGS; DYT3; DYT3/TAF1; KAT4; MRXS33; N-TAF1; NSCL2; OF; P250; TAF(II)250; TAF2A; TAFII-250; TAFII250; XDP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG240220 representing NM_001286074.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGACCCGGCTGCGATTTGCTGCTGCGGACAGCAGCTACCATCACTGCTGCCGCCATCATGTCAGAC
  ACGGACAGCGACGAAGATTCCGCTGGAGGCGGCCCATTTTCTTTAGCGGGTTTCCTTTTCGGCAACATC
  AATGGAGCCGGGCAGCTGGAGGGGGAAAGCGTCTTGGATGATGAATGTAAGAAGCACTTGGCAGGCTTG
  GGGGCTTTGGGGCTGGGCAGCCTGATCACTGAACTCACGGCAAATGAAGAATTGACCGGGACTGACGGT
  GCCTTGGTAAATGATGAAGGGTGGGTTAGGAGTACAGAAGATGCTGTGGACTATTCAGACATCAATGAG
  GTGGCAGAAGATGAAAGCCGAAGATACCAGCAGACGATGGGGAGCTTGCAGCCCCTTTGCCACTCAGAT
  TATGATGAAGATGACTATGATGCTGATTGTGAAGACATTGATTGCAAGTTGATGCCTCCTCCACCTCCA
  CCCCCGGGACCAATGAAGAAGGATAAGGACCAGGATTCTATTACTGGTGTGTCTGAAAATGGAGAAGGC
  ATCATCTTGCCCTCCATCATTGCCCCTTCCTCTTTGGCCTCAGAGAAAGTGGACTTCAGTAGTTCCTCT
  GACTCAGAATCTGAGATGGGACCTCAGGAAGCAACACAGGCAGAATCTGAAGATGGAAAGCTGACCCTT
  CCATTGGCTGGGATTATGCAGCATGATGCCACCAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAA
  TTTCGACCTGGAAAGGTGTTACGTTTTCTACGTCTTTTTGGACCAGGGAAGAATGTCCCATCTGTTTGG
  CGGAGTGCTCGGAGAAAGAGGAAGAAGAAGCACCGTGAGCTGATACAGGAAGAGCAGATCCAGGAGGTG
  GAGTGCTCAGTAGAATCAGAAGTCAGCCAGAAGTCTTTGTGGAACTACGACTACGCTCCACCACCACCT
  CCAGAGCAGTGTCTCTCTGATGATGAAATCACGATGATGGCTCCTGTGGAGTCCAAATTTTCCCAATCA
  ACTGGAGATATAGATAAAGTGACAGATACCAAACCAAGAGTGGCTGAGTGGCGTTATGGGCCTGCCCGA
  CTGTGGTATGATATGCTGGGTGTCCCTGAAGATGGCAGTGGGTTTGACTATGGCTTCAAACTGAGAAAG
  ACAGAACATGAACCTGTGATAAAATCTAGAATGATAGAGGAATTTAGGAAACTTGAGGAAAACAATGGC
  ACTGATCTTCTGGCTGATGAAAACTTCCTGATGGTGACACAGCTGCATTGGGAGGATGATATCATCTGG
  GATGGGGAGGATGTCAAACACAAAGGGACAAAACCTCAGCGTGCAAGCCTGGCAGGCTGGCTTCCTTCT
  AGCATGACTAGGAATGCGATGGCTTACAATGTTCAGCAAGGTTTTGCAGCCACTCTTGATGATGACAAA
  CCTTGGTACTCCATTTTTCCCATTGACAATGAGGATCTGGTATATGGACGCTGGGAGGACAATATCATT
  TGGGATGCTCAGGCCATGCCCCGGCTGTTGGAACCTCCTGTTTTGACACTTGATCCCAATGATGAGAAC
  CTCATTTTGGAAATTCCTGATGAGAAGGAAGAGGCCACCTCTAACTCCCCCTCCAAGGAGAGTAAGAAG
  GAATCATCTCTGAAGAAGAGTCGAATTCTCTTAGGGAAAACAGGAGTCATCAAGGAGGAACCACAGCAG
  AACATGTCTCAGCCAGAAGTGAAAGATCCATGGAATCTCTCCAATGATGAGTATTATTATCCCAAGCAA
  CAGGGTCTTCGAGGCACCTTTGGAGGGAATATTATCCAGCATTCAATTCCTGCTGTGGAATTACGGCAG
  CCCTTCTTTCCCACCCACATGGGGCCCATCAAACTCCGGCAGTTCCATCGCCCACCTCTGAAAAAGTAC
  TCATTTGGTGCACTTTCTCAGCCAGGTCCCCACTCAGTCCAACCTTTGCTAAAGCACATCAAAAAAAAG
  GCCAAGATGAGAGAACAAGAGAGGCAAGCTTCAGGTGGTGGAGAGATGTTTTTTATGCGCACACCTCAG
  GACCTCACAGGCAAAGATGGTGATCTTATTCTTGCAGAATATAGTGAGGAAAATGGACCCTTAATGATG
  CAGGTTGGCATGGCAACCAAGATAAAGAACTATTATAAACGGAAACCTGGAAAAGATCCTGGAGCACCA
  GATTGTAAATATGGGGAAACTGTTTACTGCCATACATCTCCTTTCCTGGGTTCTCTCCATCCTGGCCAA
  TTGCTGCAAGCATTTGAGAACAACCTTTTTCGTGCTCCAATTTATCTTCATAAGATGCCAGAAACTGAT
  TTCTTGATCATTCGGACAAGACAGGGTTACTATATTCGGGAATTAGTGGATATTTTTGTGGTTGGCCAG
  CAGTGTCCCTTGTTTGAAGTTCCTGGGCCTAACTCCAAAAGGGCCAATACGCATATTCGAGACTTTCTA
  CAGGTTTTTATTTACCGCCTTTTCTGGAAAAGTAAAGATCGGCCACGGAGGATACGAATGGAAGATATA
  AAAAAAGCCTTTCCTTCCCATTCAGAAAGCAGCATCCGGAAGAGGCTAAAGCTCTGCGCTGACTTCAAA
  CGCACAGGGATGGACTCAAACTGGTGGGTGCTTAAGTCTGATTTTCGTTTACCAACGGAAGAAGAGATC
  AGAGCTATGGTGTCACCAGAGCAGTGCTGTGCTTATTATAGCATGATAGCTGCAGAGCAACGACTGAAG
  GATGCTGGCTATGGTGAGAAATCCTTTTTTGCTCCAGAAGAAGAAAATGAGGAAGATTTCCAGATGAAG
  ATTGATGATGAAGTTCGCACTGCCCCTTGGAACACCACAAGGGCCTTCATTGCTGCCATGAAGGGCAAG
  TGTCTGCTAGAGGTGACTGGGGTGGCAGATCCCACGGGGTGTGGTGAAGGATTCTCCTATGTGAAGATT
  CCAAACAAACCAACACAGCAGAAGGATGATAAAGAACCGCAGCCAGTGAAGAAGACAGTGACAGGAACA
  GATGCAGACCTTCGTCGCCTTTCCCTGAAAAATGCCAAGCAACTTCTACGTAAATTTGGTGTGCCTGAG
  GAAGAGATTAAAAAGTTGTCCCGCTGGGAAGTGATTGATGTGGTGCGCACAATGTCAACAGAACAGGCT
  CGTTCTGGAGAGGGGCCCATGAGTAAATTTGCCCGTGGATCAAGGTTTTCTGTGGCTGAGCATCAAGAG
  CGTTACAAAGAGGAATGTCAGCGCATCTTTGACCTACAGAACAAGGTTCTGTCATCAACTGAAGTCTTA
  TCAACTGACACAGACAGCAGCTCAGCTGAAGATAGTGACTTTGAAGAAATGGGAAAGAACATTGAGAAC
  ATGTTGCAGAACAAGAAAACCAGCTCTCAGCTTTCACGTGAACGGGAGGAACAGGAGCGGAAGGAACTA
  CAGCGAATGCTACTGGCAGCAGGCTCAGCAGCATCCGGAAACAATCACAGAGATGATGACACAGCTTCC
  GTGACTAGCCTTAACTCTTCTGCCACTGGACGCTGTCTCAAGATTTATCGCACGTTTCGAGATGAAGAG
  GGGAAAGAGTATGTTCGCTGTGAGACAGTCCGAAAACCAGCTGTCATTGATGCCTATGTGCGCATACGG
  ACTACAAAAGATGAGGAATTCATTCGAAAATTTGCCCTTTTTGATGAACAACATCGGGAAGAGATGCGA
  AAAGAACGGCGGAGGATTCAAGAGCAACTGAGGCGGCTTAAGAGGAACCAGGAAAAGGAGAAGCTTAAG
  GGTCCTCCTGAGAAGAAGCCCAAGAAAATGAAGGAGCGTCCTGACCTAAAACTGAAATGTGGGGCATGT
  GGTGCCATTGGACACATGAGGACTAACAAATTCTGCCCCCTCTATTATCAAACAAATGCGCCACCTTCC
  AACCCTGTTGCCATGACAGAAGAACAGGAGGAGGAGTTGGAAAAGACAGTCATTCATAATGATAATGAA
  GAACTTATCAAGGTTGAAGGGACCAAAATTGTCTTGGGGAAACAGCTAATTGAGAGTGCGGATGAGGTT
  CGCAGAAAATCTCTGGTTCTCAAGTTTCCTAAACAGCAGCTTCCTCCAAAGAAGAAACGGCGAGTTGGA
  ACCACTGTTCACTGTGACTATTTGAATAGACCTCATAAGTCCATCCACCGGCGCCGCACAGACCCTATG
  GTGACGCTGTCGTCCATCTTGGAGTCTATCATCAATGACATGAGAGATCTTCCAAATACATACCCTTTC
  CACACTCCAGTCAATGCAAAGGTTGTAAAGGACTACTACAAAATCATCACTCGGCCAATGGACCTACAA
  ACACTCCGCGAAAACGTGCGTAAACGCCTCTACCCATCTCGGGAAGAGTTCAGAGAGCATCTGGAGCTA
  ATTGTGAAAAATAGTGCAACCTACAATGGGCCAAAACACTCATTGACTCAGATCTCTCAATCCATGCTG
  GATCTCTGTGATGAAAAACTCAAAGAGAAAGAAGACAAATTAGCTCGCTTAGAGAAAGCTATCAACCCC
  TTGCTGGATGATGATGACCAAGTGGCGTTTTCTTTCATTCTGGACAACATTGTCACCCAGAAAATGATG
  GCAGTTCCAGATTCTTGGCCATTTCATCACCCAGTTAATAAGAAATTTGTTCCAGATTATTACAAAGTG
  ATTGTCAATCCAATGGATTTAGAGACCATACGTAAGAACATCTCCAAGCACAAGTATCAGAGTCGGGAG
  AGCTTTCTGGATGATGTAAACCTTATTCTGGCCAACAGTGTTAAGTATAATGGACCTGAGAGTCAGTAT
  ACTAAGACTGCCCAGGAGATTGTGAACGTCTGTTACCAGACATTGACTGAGTATGATGAACATTTGACT
  CAACTTGAGAAGGATATTTGTACTGCTAAAGAAGCAGCTTTGGAGGAAGCAGAATTAGAAAGCCTGGAC
  CCAATGACCCCAGGGCCCTACACGCCTCAGGCTAAGCCTCCTGATTTGTATGATACCAACACATCCCTC
  AGTATGTCTCGAGATGCCTCTGTATTTCAAGATGAGAGCAATATGTCTGTCTTGGATATTCCCAGTGCC
  ACTCCAGAAAAGCAGGTAACACAGGAAGGTGAAGATGGAGATGGTGATCTTGCAGATGAAGAGGAAGGA
  ACTGTACAACAGCCTCAAGCCAGTGTCCTGTATGAGGATTTGCTTATGTCTGAAGGAGAAGATGATGAG
  GAAGATGCTGGGAGTGATGAAGAAGGAGACAATCCTTTCTCTGCTATCCAGCTGAGTGAAAGTGGAAGT
  GACTCTGATGTGGGATCTGGTGGAATAAGACCCAAACAACCCCGCATGCTTCAGGAGAACACAAGGATG
  GACATGGAAAATGAAGAAAGCATGATGTCCTATGAGGGAGACGGTGGGGAGGCTTCCCATGGTTTGGAG
  GATAGCAACATCAGTTATGGGAGCTATGAGGAGCCTGATCCCAAGTCGAACACCCAAGACACAAGCTTC
  AGCAGCATCGGTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAGGAGGAAGAGCAGCGCTCTGGG
  CCGAGCGTACTAAGCCAGGTCCACCTGTCAGAGGACGAGGAGGACAGTGAGGATTTCCACTCCATTGCT
  GGGGACAGTGACTTGGACTCTGATGAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG240220
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGPGCDLLLRTAATITAAAIMSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGL
  GALGLGSLITELTANEELTGTDGALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHSD
  YDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDSITGVSENGEGIILPSIIAPSSLASEKVDFSSSS
  DSESEMGPQEATQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVW
  RSARRKRKKKHRELIQEEQIQEVECSVESEVSQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQS
  TGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKTEHEPVIKSRMIEEFRKLEENNG
  TDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDK
  PWYSIFPIDNEDLVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKESKK
  ESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQ
  PFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQ
  DLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQ
  LLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFL
  QVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEI
  RAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGK
  CLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPE
  EEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEVL
  STDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTAS
  VTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMR
  KERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPS
  NPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVG
  TTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQ
  TLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINP
  LLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVNPMDLETIRKNISKHKYQSRE
  SFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLD
  PMTPGPYTPQAKPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPSATPEKQVTQEGEDGDGDLADEEEG
  TVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRM
  DMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEEQRSG
  PSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286074
• ORF Size: 5685 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001286074.1, NP_001273003.1
• RefSeq Size: 7740 bp
• RefSeq ORF: 5628 bp
• Locus ID: 6872
• UniProt ID: P21675
• Cytogenetics: Xq13.1
• Protein Families: Protein Kinase
• Protein Pathways: Basal transcription factors
• MW: 215.4 kDa
• Gene Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]