PIP5K1 beta (PIP5K1B) (NM_001278253) Human Tagged ORF Clone

CAT#: RG238592

PIP5K1B (tGFP-tagged) - Human phosphatidylinositol-4-phosphate 5-kinase, type I, beta (PIP5K1B), transcript variant 3

Product Images

Circular map for RG238592

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PIP5K1 beta
• Synonyms: MSS4; STM7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238592 representing NM_001278253.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTTCTGCTGCTGAAAATGGAGAGGCAGCACCTGGAAAACAAAATGAAGAAAAAACCTATAAAAAG
  ACTGCATCATCTGCTATTAAAGGTGCTATTCAGCTGGGAATAGGATACACAGTGGGTAATCTCACTTCC
  AAGCCAGAACGAGATGTTCTTATGCAAGACTTTTATGTGGTGGAAAGTGTGTTCCTACCCAGCGAAGGG
  AGCAATCTGACCCCAGCACATCACTACCCAGACTTTAGATTTAAGACATACGCTCCATTAGCATTCCGA
  TATTTCAGAGAACTTTTTGGTATCAAGCCTGATGATTACTTGTATTCCATCTGCAGTGAACCTCTAATA
  GAACTGTCTAACCCTGGAGCCAGTGGATCCTTGTTTTTTGTGACCAGTGATGATGAATTTATCATCAAA
  ACAGTTCAGCACAAAGAAGCTGAGTTTCTTCAGAAGCTACTGCCAGGCTATTACATGAATTTAAACCAG
  AATCCAAGGACTCTTTTGCCAAAATTTTACGGACTGTATTGTATGCAATCAGGAGGCATTAATATCAGG
  ATTGTGGTGATGAACAACGTTTTGCCACGCTCCATGAGAATGCACTTTACATATGACTTGAAAGGCTCA
  ACGTATAAGCGAAGAGCATCCCGTAAAGAGAGAGAGAAATCCAACCCCACATTTAAGGACTTAGATTTC
  CTGCAAGACATGCACGAAGGGTTGTATTTTGATACGGAAACATACAACGCGCTTATGAAAACACTTCAG
  AGAGACTGCCGGGTGCTAGAAAGCTTCAAGATCATGGATTATAGCCTTCTGTTGGGAATTCATTTCCTG
  GACCATTCCCTCAAAGAGAAAGAGGAGGAGACCCCACAAAATGTGCCTGATGCTAAGCGGACTGGGATG
  CAGAAGGTTCTCTACTCAACAGCCATGGAATCTATCCAGGGTCCAGGGAAATCTGGAGATGGGATAATC
  ACAGAGAACCCAGACACAATGGGAGGCATTCCAGCTAAAAGCCATAGGGGAGAAAAACTACTTTTATTT
  ATGGGCATTATTGACATTCTGCAATCATATAGGTTAATGAAGAAGTTAGAACATTCCTGGAAAGCTCTT
  GTTTATGATGGGGACACTGTTTCTGTTCATAGACCAAGCTTTTATGCAGACAGATTTCTTAAGTTCATG
  AATTCCAGAGTTTTCAAGAAAATTCAAGCTTTGAAGGCTTCACCGTCTAAGAAACGGTGCAATTCAATC
  GCCGCCCTAAAGGCCACTTCACAGGAGATTGTGTCCTCAATTAGCCAGGAATGGAAGGATGAGAAGCGG
  GATTTGCTGACTGAAGGACAAAGTTTTAGCAGCCTTGATGAAGAAGCCCTGGGATCCCGACACAGGCCA
  GACCTGGTCCCTAGCACTCCATCACTGTTTGAAGCTGCTTCCTTGGCAACCACAATTTCATCTTCTTCC
  TTATACGTCAATGAGCACTATCCACACGACAGGCCTACACTCTATTCAAACAGTAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238592
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSSAAENGEAAPGKQNEEKTYKKTASSAIKGAIQLGIGYTVGNLTSKPERDVLMQDFYVVESVFLPSEG
  SNLTPAHHYPDFRFKTYAPLAFRYFRELFGIKPDDYLYSICSEPLIELSNPGASGSLFFVTSDDEFIIK
  TVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCMQSGGINIRIVVMNNVLPRSMRMHFTYDLKGS
  TYKRRASRKEREKSNPTFKDLDFLQDMHEGLYFDTETYNALMKTLQRDCRVLESFKIMDYSLLLGIHFL
  DHSLKEKEEETPQNVPDAKRTGMQKVLYSTAMESIQGPGKSGDGIITENPDTMGGIPAKSHRGEKLLLF
  MGIIDILQSYRLMKKLEHSWKALVYDGDTVSVHRPSFYADRFLKFMNSRVFKKIQALKASPSKKRCNSI
  AALKATSQEIVSSISQEWKDEKRDLLTEGQSFSSLDEEALGSRHRPDLVPSTPSLFEAASLATTISSSS
  LYVNEHYPHDRPTLYSNSK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278253
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001278253.1, NP_001265182.1
• RefSeq Size: 2682 bp
• RefSeq ORF: 1509 bp
• Locus ID: 8395
• UniProt ID: O14986
• Cytogenetics: 9q21.11
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
• MW: 57.4 kDa
• Gene Summary: Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2. Together with PIP5K1A is required after stimulation of G-protein coupled receptors for stable platelet adhesion (By similarity).[UniProtKB/Swiss-Prot Function]