CysLT1 (CYSLTR1) (NM_001282187) Human Tagged ORF Clone

CAT#: RG237547

CYSLTR1 (tGFP-tagged) - Human cysteinyl leukotriene receptor 1 (CYSLTR1), transcript variant 1

Product Images

Circular map for RG237547

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CysLT1
• Synonyms: CYSLT1; CYSLT1R; CYSLTR; HMTMF81
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237547 representing NM_001282187.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATGAAACAGGAAATCTGACAGTATCTTCTGCCACATGCCATGACACTATTGATGACTTCCGCAAT
  CAAGTGTATTCCACCTTGTACTCTATGATCTCTGTTGTAGGCTTCTTTGGCAATGGCTTTGTGCTCTAT
  GTCCTCATAAAAACCTATCACAAGAAGTCAGCCTTCCAAGTATACATGATTAATTTAGCAGTAGCAGAT
  CTACTTTGTGTGTGCACACTGCCTCTCCGTGTGGTCTATTATGTTCACAAAGGCATTTGGCTCTTTGGT
  GACTTCTTGTGCCGCCTCAGCACCTATGCTTTGTATGTCAACCTCTATTGTAGCATCTTCTTTATGACA
  GCCATGAGCTTTTTCCGGTGCATTGCAATTGTTTTTCCAGTCCAGAACATTAATTTGGTTACACAGAAA
  AAAGCCAGGTTTGTGTGTGTAGGTATTTGGATTTTTGTGATTTTGACCAGTTCTCCATTTCTAATGGCC
  AAACCACAAAAAGATGAGAAAAATAATACCAAGTGCTTTGAGCCCCCACAAGACAATCAAACTAAAAAT
  CATGTTTTGGTCTTGCATTATGTGTCATTGTTTGTTGGCTTTATCATCCCTTTTGTTATTATAATTGTC
  TGTTACACAATGATCATTTTGACCTTACTAAAAAAATCAATGAAAAAAAATCTGTCAAGTCATAAAAAG
  GCTATAGGAATGATCATGGTCGTGACCGCTGCCTTTTTAGTCAGTTTCATGCCATATCATATTCAACGT
  ACCATTCACCTTCATTTTTTACACAATGAAACTAAACCCTGTGATTCTGTCCTTAGAATGCAGAAGTCC
  GTGGTCATAACCTTGTCTCTGGCTGCATCCAATTGTTGCTTTGACCCTCTCCTATATTTCTTTTCTGGG
  GGTAACTTTAGGAAAAGGCTGTCTACATTCAGAAAGCATTCTTTGTCCAGCGTGACTTATGTACCCAGA
  AAGAAGGCCTCTTTGCCAGAAAAAGGAGAAGAAATATGTAAAGTA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237547
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDETGNLTVSSATCHDTIDDFRNQVYSTLYSMISVVGFFGNGFVLYVLIKTYHKKSAFQVYMINLAVAD
  LLCVCTLPLRVVYYVHKGIWLFGDFLCRLSTYALYVNLYCSIFFMTAMSFFRCIAIVFPVQNINLVTQK
  KARFVCVGIWIFVILTSSPFLMAKPQKDEKNNTKCFEPPQDNQTKNHVLVLHYVSLFVGFIIPFVIIIV
  CYTMIILTLLKKSMKKNLSSHKKAIGMIMVVTAAFLVSFMPYHIQRTIHLHFLHNETKPCDSVLRMQKS
  VVITLSLAASNCCFDPLLYFFSGGNFRKRLSTFRKHSLSSVTYVPRKKASLPEKGEEICKV
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282187
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001282187.2
• RefSeq Size: 2863 bp
• RefSeq ORF: 1014 bp
• Locus ID: 10800
• UniProt ID: Q9Y271
• Cytogenetics: Xq21.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• MW: 38.5 kDa
• Gene Summary: This gene encodes a member of the G-protein coupled receptor 1 family. The encoded protein is a receptor for cysteinyl leukotrienes, and is involved in mediating bronchoconstriction via activation of a phosphatidylinositol-calcium second messenger system. Activation of the encoded receptor results in contraction and proliferation of bronchial smooth muscle cells, eosinophil migration, and damage to the mucus layer in the lung. Upregulation of this gene is associated with asthma and dysregulation may also be implicated in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]